Palmitoylethanolamide and Polydatin combination reduces inflammation and oxidative stress in vascular injury

Gugliandolo, Enrico; Fusco, Roberta; Biundo, Flavia; D’Amico, Ramona; Benedetto, Filippo; Paola, Rosanna Di; Cuzzocrea, Salvatore

doi:10.1016/j.phrs.2017.06.014

Cited by 61 publications

(42 citation statements)

References 25 publications

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“…In contrast to the several evidence demonstrating PEA-induced anti(neuro)inflammatory properties in AD animal models [15], there are no data on the possible involvement of antioxidant mechanisms in the beneficial action of PEA against the progression of the pathology. This is possibly due to previous data suggesting that PEA lacks direct antioxidant activity to prevent formation of free radicals and to counteract damage to DNA, lipids and proteins [43]. Other results indicate, however, that the compound enhanced the antioxidant capacity of the hippocampus in an animal model of depression [44] and decreased oxidative stress in a mouse model of diabetic peripheral neuropathy [45].…”

Section: Discussionmentioning

confidence: 85%

Chronic Oral Palmitoylethanolamide Administration Rescues Cognitive Deficit and Reduces Neuroinflammation, Oxidative Stress, and Glutamate Levels in A Transgenic Murine Model of Alzheimer’s Disease

Beggiato

Tomasini

Cassano

et al. 2020

JCM

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N-palmitoylethanolamide (PEA) is a lipid mediator belonging to the class of the N-acylethanolamine. Products containing PEA, also in ultramicronized formulation (um-PEA), are already licensed for use in humans for its analgesic and anti-inflammatory properties, and demonstrated high safety and tolerability. Preclinical studies indicate that PEA, especially in the ultramicronized form, could be a potential therapeutic agent for Alzheimer’s disease (AD). In this study, we evaluated the neuroprotective and antioxidant effects of chronic (three months) um-PEA administration in an animal model of AD (3×Tg-AD mice). For translation purposes, the compound has been orally administered. Cognitive performance as well as biochemical markers [(interleukin-16 (IL-16) and tumor necrosis factor-α (TNF-α)] levels, reactive oxygen species (ROS) production, synaptophysin and glutamate levels) have been evaluated at the end of um-PEA treatment. The results indicate that orally administered um-PEA was adsorbed and distributed in the mice brain. The chronic treatment with um-PEA (100 mg/kg/day for three months) rescued cognitive deficit, restrained neuroinflammation and oxidative stress, and reduced the increase in hippocampal glutamate levels observed in 3×Tg-AD mice. Overall, these data reinforce the concept that um-PEA exerts beneficial effects in 3×Tg-AD mice. The fact that PEA is already licensed for the use in humans strongly supports its rapid translation in clinical practice.

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Section: Discussionmentioning

confidence: 85%

Chronic Oral Palmitoylethanolamide Administration Rescues Cognitive Deficit and Reduces Neuroinflammation, Oxidative Stress, and Glutamate Levels in A Transgenic Murine Model of Alzheimer’s Disease

Beggiato

Tomasini

Cassano

et al. 2020

JCM

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“…Specially, the level of pro-inammatory factor (TNF-a) was strongly reduced by PD, this was in accordance with many other reports. 10,33,34 Besides, the adding of TNF-a counteracted PD-induced NLRP3 inammasome inactivation via enhancing NF-kB pathway. 35 These researches hent that PD may interact with TNF-a.…”

Section: Discussionmentioning

confidence: 99%

Polydatin ameliorates pulmonary fibrosis by suppressing inflammation and the epithelial mesenchymal transition via inhibiting the TGF-β/Smad signaling pathway

Qiu

Pan

2019

RSC Adv.

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Pulmonary fibrosis is a chronic and progressive lung disease which results in a loss of pulmonary function and eventually respiratory failure. Inflammation and epithelial mesenchymal transition (EMT) play important roles in the pathogenesis of pulmonary fibrosis. This study aimed to investigate the therapeutic effect of polydatin (PD) in bleomycin-induced pulmonary fibrosis. A bleomycin-induced pulmonary fibrosis animal model used SD rats. Morphological changes were analyzed by hematoxylin-eosin staining. RT-qPCR and western blot were used for the detection of the expression of TGF-b1, collagen I, collagen III, Ecadherin, fibronectin and the ratios of p-Smad2/Smad2, p-Smad3/Smad3. The concentrations of PICP, PIIINP, TNF-a, IL-1b, IL-6 and IL-17 were measured by enzyme linked immunosorbent assay (Elisa) assay. Results showed that PD attenuated bleomycin-induced pulmonary fibrosis. The beneficial effect of PD was possibly related to the inhibition of inflammation and EMT through suppressing the TGF-b/Smad signaling pathway. Our findings suggested that PD might be a potential therapeutic candidate in the treatment of pulmonary fibrosis.Fig. 4 PD suppresses TGF-b/Smad signaling pathway in pulmonary fibrosis. (A) Relative protein level of TGF-b, p-Smad2, Smad2, p-Smad3, Smad3 was detected by Western blot. (B-D) The ratios of TGF-b, p-Smad2/Smad2 and p-Smad3/Smad3 in different groups. All data were represented as the mean AE SD from three independent experiments. **P < 0.01 compared with control group, # P < 0.05, ## P < 0.01 compared with TGF-b group.8110 | RSC Adv., 2019,9,[8104][8105][8106][8107][8108][8109][8110][8111][8112] This journal is

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“…Western blots were performed as described from our previous studies [ 60 ]. Specific primary antibody: anti-NRF2 (1:600, Santa Cruz Biotechnology) or anti-iNOS (1:700; Santa Cruz Biotechnology) or anti-IkB-α (1:700; Santa Cruz Biotechnology) or anti-NF-kB (1:700; Santa Cruz Biotechnology) was mixed in 1 × PBS, 5% w/v nonfat dried milk, and 0.1% Tween 20, and were incubated at 4 °C, overnight.…”

Section: Methodsmentioning

confidence: 99%

Consumption of Anacardium occidentale L. (Cashew Nuts) Inhibits Oxidative Stress through Modulation of the Nrf2/HO−1 and NF-kB Pathways

et al. 2020

Self Cite

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Ischemia/reperfusion injury is a severe disorder associated with a high mortality. Several antioxidant and pharmacological properties of cashew nuts (Anacardium occidentale L.) and its metabolites from different countries have recently been described. It is a medicinal plant with important therapeutic effects. This study aimed to verify the effect of an oral administration of cashew nuts in a rat model of ischemia/reperfusion (I/R). Adult male rats were subjected to intestinal I/R injury by clamping the superior mesenteric artery for 30 min and then allowing animals to 1 h of reperfusion. Rats subjected to I/R of the gut showed a significant increase in different biochemical markers. In particular, we evaluated lipid peroxidation, tissue myeloperoxidase activity, protein carbonyl content, reactive oxygen species generation and decreased antioxidant enzyme activities. Western blot analysis showed the activation of the NRF2 and NF-kB pathways. Increased immunoreactivity to nitrotyrosine, PARP, P-selectin, and ICAM-1 was observed in the ileum of rats subjected to I/R. Administration of cashew nuts (100 mg/kg) significantly reduced the mortality rate, the fall in arterial blood pressure, and oxidative stress and restored the antioxidant enzyme activities by a mechanism involving both NRF2 and NF-kB pathways. Cashew nuts treatments reduced cytokines plasma levels, nitrotyrosine, and PARP expression as well as adhesion molecules expressions. Additionally, cashew nuts decreased the intestinal barrier dysfunction and mucosal damage, the translocation of toxins and bacteria, which leads to systemic inflammation and associated organs injuries in particular of liver and kidney. Our study demonstrates that cashew nuts administration exerts antioxidant and pharmacological protective effects in superior mesenteric artery occlusion–reperfusion shock.

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Palmitoylethanolamide and Polydatin combination reduces inflammation and oxidative stress in vascular injury

Cited by 61 publications

References 25 publications

Chronic Oral Palmitoylethanolamide Administration Rescues Cognitive Deficit and Reduces Neuroinflammation, Oxidative Stress, and Glutamate Levels in A Transgenic Murine Model of Alzheimer’s Disease

Chronic Oral Palmitoylethanolamide Administration Rescues Cognitive Deficit and Reduces Neuroinflammation, Oxidative Stress, and Glutamate Levels in A Transgenic Murine Model of Alzheimer’s Disease

Polydatin ameliorates pulmonary fibrosis by suppressing inflammation and the epithelial mesenchymal transition via inhibiting the TGF-β/Smad signaling pathway

Consumption of Anacardium occidentale L. (Cashew Nuts) Inhibits Oxidative Stress through Modulation of the Nrf2/HO−1 and NF-kB Pathways

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