Panulirus argus virus 1 (PaV1) infection prevalence and risk factors in a Mexican lobster fishery employing casitas

Huchin-Mian, Juan Pablo; Rodríguez-Canul, Rossanna; Briones‐Fourzán, Patricia; Lozano‐Álvarez, Enrique

doi:10.3354/dao02676

Cited by 17 publications

(21 citation statements)

References 34 publications

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“…The DNA sequence obtained herein, showed a high homology to a similar DNA sequence of PaV1 from the GenBank™, confirming that the amplified PCR products described here were from DNA of PaV1 infected lobsters. In this sense, it has been reported that lobster surveys based on observed clinical disease has underestimated the prevalence of PaV1 infection, especially in early-stage of subclinical infections because the clinical signs appear as PaV1 infection progresses [23]. For example, in Quintana Roo, Mexico, the prevalence of PaV1 by clinical signs was higher among lobsters at Punta Allen (8.4%, n = 1842) compared to Vigía Chico (1.5%, n = 2016) [37].…”

Section: Discussionmentioning

confidence: 99%

“…Regarding to the PaV1-PCR; it has been validated previously in diseased spiny lobsters P. argus with and without clinical signs of PaV1, and it has been also used to assess PaV1 prevalence in frozen lobsters tails intended for commercialization, thus the risk of non-specificity is very low [23] [26]. The DNA sequence obtained herein, showed a high homology to a similar DNA sequence of PaV1 from the GenBank™, confirming that the amplified PCR products described here were from DNA of PaV1 infected lobsters.…”

Section: Discussionmentioning

confidence: 99%

“…After being centrifuged at 3000 g for 3 min, a supernatant fluid containing DNA was carefully transferred into a sterile tube and stored at −20˚C. DNA quality and quantity was confirmed by determining the absorbance ratio A260:A280 using a NanoDrop 2000c spectrophotometer (Thermo-Scientific), and chromosomal DNA integrity was assessed by resolving DNA in 1% agarose gels [23]. The quality of genomic DNA was assessed by amplifying the small subunit ribosomal RNA (SSU rRNA) of the lobster using "universal" SSU rRNA primers modified from Medlin et al (1988) [24].…”

Section: Dna Extraction Pcr Screening For Pav1 and Dna Sequencementioning

confidence: 99%

“…Soon after sampling, the exoskeleton of each lobster was swabbed with 70% ethanol, and ~300 µl of hemolymph was withdrawn from the base of one of the 5th periopod using a sterile 1 ml disposable syringe fitted with a 30 gauge needle. Hemolymph was immediately fixed in 95% ethanol (ratio 1:3 v/v), divided into aliquots of 300 µl and stored in a cooler containing frozen refrigerant packs until stored at −80˚C [23].…”

Section: Sample and Data Collectionmentioning

confidence: 99%

“…Visualization was done using UV illumination (MiniBis Pro ® ). The negative control was DNA from uninfected lobsters and ultrapure water, and the positive control was DNA from lobsters heavily infected with PaV1 [23] [25] [26]. The PCR was performed in triplicate to avoid non-specific results.…”

Section: Dna Extraction Pcr Screening For Pav1 and Dna Sequencementioning

confidence: 99%

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Landscape Analysis for PaV1 Infection in Lobsters Panulirus argus from the Artisanal Fishery of the Eastern Coast of Yucatan, Mexico

Pérez-Campos¹,

Huchim-Lara²,

Salas³

et al. 2016

OJMS

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Panulirus argus virus 1 (PaV1) is considered a major threat to spiny lobsters Panulirus argus. In this study Geospatial analysis was used to analyze PaV1 distribution in an artisanal fishery of spiny lobster Panulirus argus population from the north coast of the Yucatan Peninsula. Adult and sub-adult P. argus and seabed coverage data were collected from thirty artisanal fishing sites. Five seabed coverage types were identified: seagrass; sand/seagrass mixture; sand only; coral/sand mixture; and seaweed. No juveniles were examined. Of the 358 collected lobsters, PaV1 was identified in four organisms (three sub-adults and one adult) from two fishing sites (termed A & B), both found in a seagrass coverage area. Overall prevalence was of 1.12%. Prevalence was of 20% (2/10) at one site and of 12.6% (2/16) at the other.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Dna Extraction Pcr Screening For Pav1 and Dna Sequencementioning

confidence: 99%

Section: Sample and Data Collectionmentioning

confidence: 99%

Section: Dna Extraction Pcr Screening For Pav1 and Dna Sequencementioning

confidence: 99%

See 3 more Smart Citations

Landscape Analysis for PaV1 Infection in Lobsters Panulirus argus from the Artisanal Fishery of the Eastern Coast of Yucatan, Mexico

Pérez-Campos¹,

Huchim-Lara²,

Salas³

et al. 2016

OJMS

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Prevention and Management of Infectious Diseases in Aquatic Invertebrates

Shields

2017

Ecology of Invertebrate Diseases

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Untangling the effects of size, habitat and invertebrate biodiversity on parasite prevalence in the Caribbean spiny lobster

2019

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The spiny lobster Panulirus argus is an important benthic mesopredator and a major fishing resource across the Wider Caribbean region. This species is host to the pathogenic virus PaV1 and metacercariae of Cymatocarpus solearis, a digenean trematode whose first intermediate host remains unknown. Previous studies found that the probability of infection with PaV1 was higher in juvenile lobsters and in densely vegetated habitats (suggesting that marine vegetation can be an environmental reservoir for PaV1), whereas the probability of infection with C. solearis was higher for larger lobsters and in poorly vegetated habitats. To increase insight into the role of habitat and body size in the ecology of lobster diseases, the presence of both C. solearis and PaV1 in P. argus was investigated across three contrasting zones in Bahía de la Ascensión, Mexico (19°35′27″N, 87°38′06″W): reef, lagoon and shallow habitat. Additionally, habitat complexity, cover of benthic components, and macroinvertebrate biodiversity were characterized in each zone. Consistent with previous studies, probability of infection with PaV1 (both at a clinical and infective level) decreased with increasing lobster size and was highest in the seagrass-rich lagoon, supporting the idea that marine vegetation could be an environmental reservoir for PaV1. In contrast, the probability of infection with C. solearis increased significantly with lobster size but did not vary with zone, suggesting no relationship with benthic substrate type. However, based on results of macroinvertebrate diversity, the gastropods Cerithium litteratum and Tegula fasciata are put forward as potential candidates for the first intermediate hosts of C. solearis.

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Panulirus argus virus 1 (PaV1) infection prevalence and risk factors in a Mexican lobster fishery employing casitas

Cited by 17 publications

References 34 publications

Landscape Analysis for PaV1 Infection in Lobsters Panulirus argus from the Artisanal Fishery of the Eastern Coast of Yucatan, Mexico

Landscape Analysis for PaV1 Infection in Lobsters Panulirus argus from the Artisanal Fishery of the Eastern Coast of Yucatan, Mexico

Prevention and Management of Infectious Diseases in Aquatic Invertebrates

Untangling the effects of size, habitat and invertebrate biodiversity on parasite prevalence in the Caribbean spiny lobster

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