Paper-based amperometric sensor for determination of acetylcholinesterase using screen-printed graphene electrode

Panraksa, Yosita; Siangproh, Weena; Khampieng, Thitikan; Chailapakul, Orawon; Apilux, Amara

doi:10.1016/j.talanta.2017.08.096

Cited by 85 publications

(42 citation statements)

References 46 publications

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“…The detection limit can be as low as 0.16 mU mL À1 via the uorescence recovery of RhB, which was comparable to that of recently reported assays for AChE. [17][18][19] Selectivity of the assay for AChE detection…”

Section: Sensitivity Of the Assay For Ache Detectionsupporting

confidence: 79%

“…To this end, a range of methods/techniques for detection of AChE level have been employed, including the colorimetric Ellman method 8,9 or the detection of hydrogen peroxide (H 2 O 2 ) produced by oxidation of choline. 10,11 With the development of H 2 O 2 sensors, [12][13][14] recently, uorescent or colorimetric approaches based on synthetic probes and nanozymes, [15][16][17][18] as well as various electrochemical methods, 19,20 have been developed to monitor AChE activity and screen the inhibitors of AChE. However, most of these methods cannot discriminate AChE from other biothiols.…”

Section: Introductionmentioning

confidence: 99%

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A gold nanoparticle based colorimetric and fluorescent dual-channel probe for acetylcholinesterase detection and inhibitor screening

Wang

et al. 2018

RSC Adv.

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Section: Sensitivity Of the Assay For Ache Detectionsupporting

confidence: 79%

Section: Introductionmentioning

confidence: 99%

A gold nanoparticle based colorimetric and fluorescent dual-channel probe for acetylcholinesterase detection and inhibitor screening

Wang

et al. 2018

RSC Adv.

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“…61 Graphene ink was used to screen print the WE and CE onto a paper substrate, whereas the RE was screen printed using Ag/AgCl ink. A graphene screenprinted electrode was chosen over a carbon screen-printed electrode due to the higher currents displayed by graphene.…”

Section: ·1 Clinical Diagnosticsmentioning

confidence: 99%

Trends in Paper-based Electrochemical Biosensors: From Design to Application

et al. 2018

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Electrochemical bio-sensing using paper-based detection systems is the main focus of this review. The different existing designs of 2-dimensional and 3-dimensional sensors, and fabrication techniques are discussed. This review highlights the effect of adopting different sensor designs, distinct fabrication techniques, as well as different modification methods, in order to produce reliable and reproducible reading. The use of various nanomaterials have been demonstrated in order to modify the surface of electrodes during fabrication to further enhance the signal for subsequent analysis. The reviewed sensors were classified into categories based on their applications, such as diagnostics, environmental and food testing. One of the major advantages of using paper-based electrochemical sensors is the potential for miniaturization, which only requires relatively small amount of samples, and the low cost for the purpose of mass production. Additionally, most of the devices reviewed were made to be portable, making them well-suited for on-site detection. Finally, paper-based detection is an ideal platform to fabricate cost-effective, user-friendly and sensitive electrochemical biosensors, with large capacity for customization depending on functional needs.

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“…In the recent years, the miniaturization, fast response time, cheap and easy process of analytical systems made an interesting research section for their development [11]. For this aim, the paper based sensors have applied due to simple, fast, portable applicability, demanding a little sample for analysis and do not require specialized users [12][13][14]. A paper sheet has been applied as the affordable, easy to use, biodegradable substrate and enzymes could be immobilized onto the paper via physical adsorption [15,16].…”

Section: Introductionmentioning

confidence: 99%

Rapid Determination of Phenolic Compounds in Water Samples: Development of a Paper‐based Nanobiosensor Modified with Functionalized Silica Nanoparticles and Potato Tissue

et al. 2019

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In this study, we present a fast, simple, low‐cost and disposable method for determination of phenolic content in water samples, using a paper based polyphenol oxidase biosensor. The propylamine functionalized silica nanoparticles was dropped onto a paper sheet. After drying at room temperature, the potato tissue extract including polyphenol oxidase was immobilized on the paper via physical and chemical adsorption. The modified paper was placed on the top of the graphite screen printed electrode. To construct of an electrochemical nanobiosensor, the electrochemical behavior of the modified electrode in different steps was investigated by cyclic voltammetry and electrochemical impedance spectroscopy methods. After being optimized the effective parameters, the changes in the biosensor electrochemical response vs. to the different concentrations of the substrate (phenol solution) were monitored by differential pulse voltammetry and amperometry methods. The linear relationships for phenol detection were obtained in the concentration ranges of 0.01–160 μM and 0.1–300 μM with a detection limit of 0.007 μM and 0.042 μM with DPV and amperometry methods, respectively. This method was successfully used in the voltammetric determination of the phenol content in the real samples, like the river water and the wastewater of wood factory.

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Paper-based amperometric sensor for determination of acetylcholinesterase using screen-printed graphene electrode

Cited by 85 publications

References 46 publications

A gold nanoparticle based colorimetric and fluorescent dual-channel probe for acetylcholinesterase detection and inhibitor screening

A gold nanoparticle based colorimetric and fluorescent dual-channel probe for acetylcholinesterase detection and inhibitor screening

Trends in Paper-based Electrochemical Biosensors: From Design to Application

Rapid Determination of Phenolic Compounds in Water Samples: Development of a Paper‐based Nanobiosensor Modified with Functionalized Silica Nanoparticles and Potato Tissue

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