Paper-based electrochemiluminescence origami device for protein detection using assembled cascade DNA–carbon dots nanotags based on rolling circle amplification

Wu, Ludan; Ma, Chao; Zheng, Xiaoxiao; Liu, Haiyun; Yu, Jinghua

doi:10.1016/j.bios.2015.01.034

Cited by 72 publications

(23 citation statements)

References 38 publications

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“…This technique also provides an attractive substitute to the other detection techniques. Yu et al developed paper-based ECL signal amplification devices using 3D origami technique for enhanced detection of proteins [190,191]. Wu et al fabricated a microfluidic paper-based ECL origami cyto-device in which aptamer-modified Au electrodes were used as a working electrode for cancer cells capture and cyto-sensing [192].…”

Section: Detection Techniques and Applicationsmentioning

confidence: 99%

Fabrication, Flow Control, and Applications of Microfluidic Paper-Based Analytical Devices

2019

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Paper-based microfluidic devices have advanced significantly in recent years as they are affordable, automated with capillary action, portable, and biodegradable diagnostic platforms for a variety of health, environmental, and food quality applications. In terms of commercialization, however, paper-based microfluidics still have to overcome significant challenges to become an authentic point-of-care testing format with the advanced capabilities of analyte purification, multiplex analysis, quantification, and detection with high sensitivity and selectivity. Moreover, fluid flow manipulation for multistep integration, which involves valving and flow velocity control, is also a critical parameter to achieve high-performance devices. Considering these limitations, the aim of this review is to (i) comprehensively analyze the fabrication techniques of microfluidic paper-based analytical devices, (ii) provide a theoretical background and various methods for fluid flow manipulation, and iii) highlight the recent detection techniques developed for various applications, including their advantages and disadvantages.

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Section: Detection Techniques and Applicationsmentioning

confidence: 99%

Fabrication, Flow Control, and Applications of Microfluidic Paper-Based Analytical Devices

2019

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“…However, with the development of isothermal amplification technology, DNA strands can be amplified without the thermal cycling process. The isothermal amplification method includes loop-mediated isothermal amplification (LAMP) [108], helicase-dependent amplification (HDA) [109], recombinase protein amplification (RPA) [110], and rolling circle amplification (RCA) [40]. Recently, several attempts have been made to transfer isothermal amplification of nucleic acid technology to the paper microfluidics platform for POCT [4,84,96,[111][112][113].…”

Section: Nucleic Acid Amplification Testing (Naat)mentioning

confidence: 99%

“…Desirable requirements of the sensing modules include high sensitivity and accuracy without external equipment, where the two factors usually undergo a trade-off relationship, so it is important to optimize these performances in assay conditions. The sensing approaches that are currently being used include (1) colorimetric methods [32-36], (2) chemiluminescence [3,37], (3) electrochemistry [38], (4) fluorescence [39], and (5) electrochemiluminescence (ECL) [40]. Since there are apparent pros and cons depending on the sensing method used, it is important to choose a sensing scheme appropriate for diagnostic specifications and applications.…”

mentioning

confidence: 99%

Recent Advances of Fluid Manipulation Technologies in Microfluidic Paper-Based Analytical Devices (μPADs) toward Multi-Step Assays

Kim¹,

Hahn

Kim

2020

Micromachines

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Microfluidic paper-based analytical devices (μPADs) have been suggested as alternatives for developing countries with suboptimal medical conditions because of their low diagnostic cost, high portability, and disposable characteristics. Recently, paper-based diagnostic devices enabling multi-step assays have been drawing attention, as they allow complicated tests, such as enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR), which were previously only conducted in the laboratory, to be performed on-site. In addition, user convenience and price of paper-based diagnostic devices are other competitive points over other point-of-care testing (POCT) devices, which are more critical in developing countries. Fluid manipulation technologies in paper play a key role in realizing multi-step assays via μPADs, and the expansion of biochemical applications will provide developing countries with more medical benefits. Therefore, we herein aimed to investigate recent fluid manipulation technologies utilized in paper-based devices and to introduce various approaches adopting several principles to control fluids on papers. Fluid manipulation technologies are classified into passive and active methods. While passive valves are structurally simple and easy to fabricate, they are difficult to control in terms of flow at a specific spatiotemporal condition. On the contrary, active valves are more complicated and mostly require external systems, but they provide much freedom of fluid manipulation and programmable operation. Both technologies have been revolutionized in the way to compensate for their limitations, and their advances will lead to improved performance of μPADs, increasing the level of healthcare around the world.

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“…They are cheap, easy, structurally flexible, and light in weight . Microfluidic paper‐based immunological analytic devices received significant attention these years for their advantages of high sensitivity, low cost, and rapid POC speed . Inside paper matrix, maneuver of reactants can be performed via gravity and capillary force, with no need to use cumbersome peripheral pumps.…”

Section: Introductionmentioning

confidence: 99%

Manual‐slide‐engaged paper chip for parallel SERS‐immunoassay measurement of clenbuterol from swine hair

et al. 2015

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Clenbuterol (CL), as a feed additive, has been banned in many countries due to its potential threat to human health. In detection of CL, a fast, low-cost technique with high accuracy and specificity would be ideal for its administrative on-field inspections. Among the attempts to pursue a reliable detection tool of CL, a technique that combines surface enhanced Raman spectroscopy (SERS) and immunoassay, is close to meet the requirements as above. However, multiple steps of interactions between CL analyte, antibody, and antigen are involved in this method, and under conventional setup, the operation of SERS/immunoassay were unwieldy. In this paper, to facilitate a more manageable sample manipulation for SERS-immunoassay measurement, a 3D paper chip was suggested. A switch-on-chip multilayered (abbreviated as SoCM-) microfluidic paper-based analysis device (μPad) was fabricated to provide operators with manual switches on the interactions between different microfluids. Besides, on a detection slip we made on the main body of our SoCM-μPad, antigen was anchored in pattern. With this architecture, multistep interactions between the CL analyte in swine hair extract and the SERS probe-modified antibody and antigen, were managed for on-chip SERS-immunoassay detection. This would be very attractive for fast, cheap, accurate, and on-site specific detection of CL from real samples.

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Paper-based electrochemiluminescence origami device for protein detection using assembled cascade DNA–carbon dots nanotags based on rolling circle amplification

Cited by 72 publications

References 38 publications

Fabrication, Flow Control, and Applications of Microfluidic Paper-Based Analytical Devices

Fabrication, Flow Control, and Applications of Microfluidic Paper-Based Analytical Devices

Recent Advances of Fluid Manipulation Technologies in Microfluidic Paper-Based Analytical Devices (μPADs) toward Multi-Step Assays

Manual‐slide‐engaged paper chip for parallel SERS‐immunoassay measurement of clenbuterol from swine hair

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