Paper-based magnetic nanoparticle-peptide probe for rapid and quantitative colorimetric detection of Escherichia coli O157:H7

Suaifan, Ghadeer A. R. Y.; Alhogail, Sahar; Zourob, Mohammed

doi:10.1016/j.bios.2016.10.023

Cited by 122 publications

(43 citation statements)

References 36 publications

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“…The synthesis of specific polypeptides is widely used in biological detection due to its advantages of reliability, economy, stability, resistance to harsh environment, and easier engineering design at the molecular level than antibodies, and is expected to replace traditional antibodies. 15 Several bioassays using peptide as substrate have been developed, such as colorimetric, [16][17][18] fluorescent, 19-22 electrochemical 23-26 and electrochemiluminescence. [27][28][29][30] The aim of this paper was to design a simple and sensitive EIS biosensor for LPS by employing a specific peptide (GIGKLHSAGKF) 31 as biological recognition molecule.…”

Section: Electrochemical Impedance Biosensor For the Determination Ofmentioning

confidence: 99%

Electrochemical Impedance Biosensor for the Determination of Lipopolysaccharide Using Peptide as the Recognition Molecule

Fan

Wang

et al. 2019

J. Braz. Chem. Soc.

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A simple and sensitive electrochemical impedance spectroscopy (EIS) biosensor was designed for the determination of lipopolysaccharide (LPS). The gold nanoparticles (GNPs) were electrochemically deposited on the glassy carbon electrode as a base electrode and the biosensor was fabricated by self-assembling a special peptide (GIGKLHSAGKF) used as molecular recognition onto the GNPs surface according to the Au-S bond. Polyethylene glycol and 6-mercapto-1-hexanol were used to block the remaining sites of the modified electrode surface. When binding of LPS, the biosensor had a good response to different concentrations of LPS and the charge transfer resistance of the biosensor was directly related to the logarithm of LPS concentrations in the range of 0.01 pg mL -1 -1.0 ng mL -1 with a limit of detection of 2.0 fg mL -1 . The characteristics of the electrochemically deposited GNPs and the optimization concentration and the self-assembly time of peptide, as well as the performance of the EIS biosensors for LPS were investigated. The biosensor was used for real serum sample determination with satisfactory results.

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Section: Electrochemical Impedance Biosensor For the Determination Ofmentioning

confidence: 99%

Electrochemical Impedance Biosensor for the Determination of Lipopolysaccharide Using Peptide as the Recognition Molecule

Fan

Wang

et al. 2019

J. Braz. Chem. Soc.

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“…Recent results for the detection of most food pathogens are summarized in Table 2 [219][220][221][222][223][238][239][240][241][242][243][244][245][246][247][248][249][250][251][252][253][254].…”

Section: Use Of Biosensors For Detection Of Foodborne Pathogens Evsmentioning

confidence: 99%

Biofilm formation and extracellular microvesicles—The way of foodborne pathogens toward resistance

Begić

Josić

2020

Electrophoresis

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Biofilm formation and extracellular microvesicles-The way of foodborne pathogens toward resistance Almost all known foodborne pathogens are able to form biofilms as one of the strategies for survival under harsh living conditions, to ward off the inhibition and the disinfection during food production, transport and storage, as well as during cleaning and sanitation of corresponding facilities. Biofilms are communities where microbial cells live under constant intracellular interaction and communication. Members of the biofilm community are embedded into extracellular matrix that contains polysaccharides, DNA, lipids, proteins, and small molecules that protect microorganisms and enable their intercellular communication under stress conditions. Membrane vesicles (MVs) are produced by both Gram positive and Gram negative bacteria. These lipid membrane-enveloped nanoparticles play an important role in biofilm genesis and in communication between different biofilm members. Furthermore, MVs are involved in other important steps of bacterial life like cell wall modeling, cellular division, and intercellular communication. They also carry toxins and virulence factors, as well as nucleic acids and different metabolites, and play a key role in host infections. After entering host cells, MVs can start many pathologic processes and cause serious harm and cell death. Prevention and inhibition of both biofilm formation and shedding of MVs by foodborne pathogens has a very important role in food production, storage, and food safety in general. Better knowledge of biofilm formation and maintaining, as well as the role of microbial vesicles in this process and in the process of host cells' infection is essential for food safety and prevention of both food spoilage and host infection.

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“…A detection limit of 6 Â 10 4 spores/g of milk powder, 2 Â 10 5 spores/g of starch, and 5 Â 10 5 spores/g of baking soda was obtained, respectively. Suaifan et al [63] described a magnetic bead-based lateral flow assay, which can specifically and simultaneously detect Escherichia coli O157:H7 proteases in complex food matrices. The limits of detection were 12 CFU/mL in broth and 30-300 CFU/mL in food matrices.…”

Section: Magnetic Beads As Labelmentioning

confidence: 99%

Current and Emerging Technologies for Rapid Detection of Pathogens

Zeng¹,

Wang²,

Hu³

2018

Biosensing Technologies for the Detection of Pathogens - A Prospective Way for Rapid Analysis

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Foodborne diseases, caused by pathogenic bacteria, have become an important social issue in the field of food safety. It presents a widespread and growing threat to human health in both developed and developing countries. As such, techniques for the detection of foodborne pathogens and waterborne pathogens are urgently needed to prevent the occurrence of human foodborne infections. Although traditional culture-based bacterial isolation and identification are the "gold standard" methods with high preciseness, their drawbacks in time-consuming are inadequate for rapid detection of pathogen to reduce foodborne disease occurrence. Fortunately, with the development of biotechnologies and nanotechnologies, various kinds of new technologies for rapid detection of pathogens have been developed so far, such as nucleic acid-based methods, antibody-based methods, and aptamer-based assays. In this chapter, we summarized the principles and the application of some recent rapid detection technologies for pathogenic bacteria. Moreover, the advantages and disadvantages of the established and emerging rapid detection methods are addressed here.

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Paper-based magnetic nanoparticle-peptide probe for rapid and quantitative colorimetric detection of Escherichia coli O157:H7

Cited by 122 publications

References 36 publications

Electrochemical Impedance Biosensor for the Determination of Lipopolysaccharide Using Peptide as the Recognition Molecule

Electrochemical Impedance Biosensor for the Determination of Lipopolysaccharide Using Peptide as the Recognition Molecule

Biofilm formation and extracellular microvesicles—The way of foodborne pathogens toward resistance

Current and Emerging Technologies for Rapid Detection of Pathogens

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