Paper-based sensor from pyrrolidinyl peptide nucleic acid for the efficient detection of Bacillus cereus

Jirakittiwut, Nuttapon; Patipong, Tanutcha; Cheiwchanchamnangij, Tawinan; Waditee‐Sirisattha, Rungaroon; Vilaivan, Tirayut; Praneenararat, Thanit

doi:10.1007/s00216-021-03633-9

Cited by 9 publications

(7 citation statements)

References 37 publications

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“…To distinguish B. cereus from other species in the Bacillus groups ( B. anthracis , B. cereus , B. thuringiensis , B. mycoides , B. pseudomycoides , and B. weihenstephanensis ) , chaperone groEL and mobility motB genes have previously been selected as robust biomarkers through bioinformatic method due to their unique sequences in B. cereus among other members of the genus 37 . For internal control, the essential 16S rRNA gene is selected due to its highly conserved sequence among the bacteria 40 .…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, acpcPNA has a more rigid structure than does aegPNA, leading to desirable properties such as better antiparallel selectivity, higher binding affinity, and better discrimination power toward single-base mismatch 32 . As such, acpcPNA has been utilized as a probe in DNA sensing studies involving various platforms 33 – 37 such as paper-based sensors, surface plasmon resonance techniques, and electrochemical techniques.…”

Section: Introductionmentioning

confidence: 99%

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Development of peptide nucleic acid-based bead array technology for Bacillus cereus detection

Noppakuadrittidej,

Charlermroj,

Makornwattana

et al. 2023

Sci Rep

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Numerous novel methods to detect foodborne pathogens have been extensively developed to ensure food safety. Among the important foodborne bacteria, Bacillus cereus was identified as a pathogen of concern that causes various food illnesses, leading to interest in developing effective detection methods for this pathogen. Although a standard method based on culturing and biochemical confirmative test is available, it is time- and labor-intensive. Alternative PCR-based methods have been developed but lack high-throughput capacity and ease of use. This study, therefore, attempts to develop a robust method for B. cereus detection by leveraging the highly specific pyrrolidinyl peptide nucleic acids (PNAs) as probes for a bead array method with multiplex and high-throughput capacity. In this study, PNAs bearing prolyl-2-aminocyclopentanecarboxylic acid (ACPC) backbone with groEL, motB, and 16S rRNA sequences were covalently coupled with three sets of fluorescently barcoded beads to detect the three B. cereus genes. The developed acpcPNA-based bead array exhibited good selectivity where only signals were detectable in the presence of B. cereus, but not for other species. The sensitivity of this acpcPNA-based bead assay in detecting genomic DNA was found to be 0.038, 0.183 and 0.179 ng for groEL, motB and 16S rRNA, respectively. This performance was clearly superior to its DNA counterpart, hence confirming much stronger binding strength of acpcPNA over DNA. The robustness of the developed method was further demonstrated by testing artificially spiked milk and pickled mustard greens with minimal interference from food metrices. Hence, this proof-of-concept acpcPNA-based bead array method has been proven to serve as an effective alternative nucleic acid-based method for foodborne pathogens.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of peptide nucleic acid-based bead array technology for Bacillus cereus detection

Noppakuadrittidej,

Charlermroj,

Makornwattana

et al. 2023

Sci Rep

Self Cite

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“…By focusing on surface antigens, knowledge of existing (active) infection can be obtained by detecting completely intact pathogens and/or cell membrane elements. An immunoglobulin analysis, which must be carried out 1-4 weeks after the infection occurred for the infected host to grow, provides a measurement of past infection [102]. Biosensors have restructured the healthcare and diagnosing industries with the growth of science and technology via the creation of cutting-edge point-ofcare gadgets [103].…”

Section: Point-of-care (Poc) Advancementsmentioning

confidence: 99%

Electrochemical and optical-based systems for SARS-COV-2 and various pathogens assessment

Ahmed,

Ansari,

Siddiqui

et al. 2023

Adv. Nat. Sci: Nanosci. Nanotechnol.

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A critical step in the process for preventing and identifying emergencies relating to health, safety, and welfare is the testing and quick diagnosis of microbial pathogens. Due to the fast spread of waterborne and food borne infections in society and the high costs associated with them, pathogen identification has emerged as one of the most difficult parts of the water and food sectors. Since the turn of the century, pathogens have demonstrated enormous epidemiological and pandemic potential. The emergence and dissemination of a novel virus with pandemic potential endanger the livelihoods and well-being of individuals worldwide. The severe acute respiratory syndrome-coronavirus-2 (SARS-COV-2) coronavirus pandemic has propagated to almost every country on Earth and has had a considerable negative influence on economies and communities. Despite improvements in identification techniques for viral diseases, all nations must now execute biosensing in a speedy, sensitive, focused, and consistent manner in order to address pressing global issues. Hence, in this review, we have critically summarised the recent advancement of electrochemical as well as optical biosensors for the monitoring of SARS-COV-2 and various pathogens. Then, we began by providing a technical overview of cutting-edge strategies utilised to combat diseases and emergencies for it, including the utilisation of point-of-care technology (POCT), artificial intelligence (AI), and the internet of medical things (IoMT). This review article explores the integration of POC, IoMT, and AI technologies in the context of personal healthcare, focusing on their potential to expedite the diagnosis and treatment of medical conditions, ultimately leading to improved patient outcomes. Subsequently, the notion and execution of multiplex testing are presented to enhance the comprehension of detecting multiple analytes. Finally, conclusions and future directions have been presented.

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“…The DNA probe was immobilized using EDC/NHS activating the reduced graphene oxide (rGO)‐AuNS‐modified electrode surface. A similar strategy was also presented by Jirakittiwut et al [ 93 ] The paper substrate was coated with carboxymethylcellulose (CMC), where the carboxyl group of the CMC was activated by the EDC–NHS reaction that allow further link with the amino group on the acpcPNA probe. Additionally, another reaction frequently used for DNA probe immobilization is a Schiff‐base reaction.…”

Section: Probe For Dna Analysismentioning

confidence: 99%

Point‐of‐Care Testing (POCT) Devices for DNA Detection: A Comprehensive Review

Mahardika,

Naorungroj,

Khamcharoen

et al. 2023

Advanced NanoBiomed Research

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DNA chips play a crucial role in point‐of‐care diagnostics by enabling rapid and accurate detection of genetic information. These chips offer high sensitivity and selectivity, allowing for the identification of specific DNA sequences associated with diseases and pathogens. Integration into lab‐on‐chip platforms streamlines and miniaturizes diagnostic workflows, paving the way for cost‐effective, portable, and user‐friendly testing devices that can revolutionize healthcare delivery. In this review, a comprehensive description of the platforms utilized in DNA analysis, including microfluidic devices and integrated DNA chips, is provided. It explores the selection and immobilization of DNA probes for improved selectivity. Additionally, it covers diverse detection techniques such as optical detection (colorimetry and fluorescence) and electrochemical techniques. In these discussions, it is aimed to provide a thorough understanding of the current state of the art in DNA biosensor‐integrated lab‐on‐chip technology for point‐of‐care testing. The continued advancements in DNA chip technology hold immense promise for the development of next‐generation point‐of‐care diagnostics, where integrated sample preparation and rapid results generation can further enhance patient outcomes and contribute to the effective management of diseases.

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Paper-based sensor from pyrrolidinyl peptide nucleic acid for the efficient detection of Bacillus cereus

Cited by 9 publications

References 37 publications

Development of peptide nucleic acid-based bead array technology for Bacillus cereus detection

Development of peptide nucleic acid-based bead array technology for Bacillus cereus detection

Electrochemical and optical-based systems for SARS-COV-2 and various pathogens assessment

Point‐of‐Care Testing (POCT) Devices for DNA Detection: A Comprehensive Review

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