Paper strip based and live cell ultrasensitive lead sensor using carbon dots synthesized from biological media

Gupta, Abhishek; Verma, Navneet Chandra; Khan, Syamantak; Tiwari, Shalini; Chaudhary, Abhishek; Nandi, Chayan Kanti

doi:10.1016/j.snb.2016.03.110

Cited by 81 publications

(35 citation statements)

References 39 publications

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“…Lead as one of the most common toxic heavy metals can be accumulated in human nervous and cardiovascular systems when exposed to contaminated air and water sources owing to its non-biodegradability [43][44][45][46], detecting for it can be realized through the PET quenching mechanism of CDs. Liu et al [46] prepared a kind of CDs with chocolate through a one-step hydrothermal method.…”

Section: Applications Of Pet Quenching Mechanisms Of Cds In Detectingmentioning

confidence: 99%

The quenching of the fluorescence of carbon dots: A review on mechanisms and applications

et al. 2017

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Carbon dots (CDs) possess unique optical properties such as tunable photoluminescence (PL) and excitation dependent multicolor emission. The quenching and recovery of the fluorescence of CDs can be utilized for detecting analytes. The PL mechanisms of CDs have been discussed in previous articles, but the quenching mechanisms of CDs have not been summarized so far. Quenching mechanisms include static quenching, dynamic quenching, Förster resonance energy transfer (FRET), photoinduced electron transfer (PET), surface energy transfer (SET), Dexter energy transfer (DET) and inner filter effect (IFE). Following an introduction, the review (with 88 refs.) first summarizes the various kinds of quenching mechanisms of CDs (including static quenching, dynamic quenching, FRET, PET and IFE), the principles of these quenching mechanisms, and the methods of distinguishing these quenching mechanisms. This is followed by an overview on applications of the various quenching mechanisms in detection and imaging.

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Section: Applications Of Pet Quenching Mechanisms Of Cds In Detectingmentioning

confidence: 99%

The quenching of the fluorescence of carbon dots: A review on mechanisms and applications

et al. 2017

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“…These high QYs were generally achieved by heteroatom (e.g., nitrogen, sulfur, phosphorus, boron) doping by using a bottom‐up synthesis approach . With such high QYs and high cell‐penetrating power, carbon dots have been widely used to image live cells . They also show superior optical properties with fluorescent intensity fluctuations at the single‐particle level, proving their potential in super‐resolution microscopy .…”

Section: Methodsmentioning

confidence: 99%

Labelling Proteins with Carbon Nanodots

et al. 2017

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We present efficient labelling of several proteins with orange-emissive carbon dots. N-Hydroxysuccinimide was used to activate the carboxyl groups of carbon dots, which subsequently reacted with the lysine groups present on the protein. Labelling was confirmed by UV absorption spectroscopy, PAGE and fluorescence correlation spectroscopy. Protein-conjugated carbon dots showed an enhancement in fluorescence lifetime and intensity owing to reduced intramolecular dynamic fluctuations. Single-molecule fluorescence measurements showed reduced fluorescence fluctuations and higher photon budget after protein tagging. Our study opens up opportunities to use carbon dots as highly precise biolabelling probes.

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“…are popularly exercised. CDs can be synthesized either from inorganic materials 19 , 33 such as graphene 34 , carbon black 35 , and candle soot 12 or biological carbon sources like food wastes, fruits, seeds and shells, plant extracts have been moving toward their application to biological sciences 36 – 38 .…”

Section: Introductionmentioning

confidence: 99%

DNA-damage and cell cycle arrest initiated anti-cancer potency of super tiny carbon dots on MCF7 cell line

Şimşek

Şüküroğlu

Yetkin

et al. 2020

Sci Rep

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While carbon-based materials have spearheaded numerous breakthroughs in biomedicine, they also have procreated many logical concerns on their overall toxicity. carbon dots (cDs) as a respectively new member have been extensively explored in nucleus directed delivery and bioimaging due to their intrinsic fluorescence properties coupled with their small size and surface properties. Although various in vitro/in vivo studies have shown that CDs are mostly biocompatible, sufficient information is lacking regarding genotoxicity of them and underlying mechanisms. This study aims to analyze the real-time cytotoxicity of super tiny CDs (2.05 ± 0.22 nm) on human breast cancer cells (MCF7) and human primary dermal fibroblast cell cultures (HDFa) by xCELLigence analysis system for further evaluating their genotoxicity and clastogenicity to evaluate the anti-tumor potential of cDs on breast adenocarcinoma. As combined with flow cytometry studies, comet assay and cytokinesisblock micronucleus assay suggest that the CDs can penetrate to the cell nuclei, interact with the genetic material, and explode DNA damage and G0/G1 phase arrest in cancer cells even at very low concentrations (0.025 ppm) which provide a strong foundation for the design of potentially promising cD-based functional nanomaterials for DnA-damage induced treatment in cancer therapy. Functional nanomaterials that can better target cancer cells that will improve prevention and therapy could be accomplished by the combined efforts of nanotoxicologists, cancer biologists and nanobiomaterial scientists focused on toxicology and related cancer therapy. Engineering carbon-based nanomaterials and their applications are among the most dynamic fields in modern advanced materials science and engineering 1-3. To date, various carbon nanomaterials such as carbon nanotubes 4,5 , fullerenes 6 , graphene 7,8 , graphene oxides 9 , carbon diamonds 10,11 , and carbon dots 12,13 have been synthesized and reported by various researchers. Among these, CDs have become particularly interesting because of their unique physical and chemical properties such as thermal and electrical conductivity, high mechanical strength together with their unique optic and fluorescence features 14-16. They have been used in bio-imaging, drug delivery, nucleus targeting, and labeling, photodynamic therapy, optoelectronics, solar cells, photocatalyst design, photodetectors, and many other biological and engineering fields 17-26. Several methods are available for the synthesis of CDs, namely, laser ablation 15 , electro-oxidation 27 or oxidative acid treatment 12 ; while nowadays, less laborsome and cheaper methods such as hydrothermal 28 and thermal synthesis 24,29,30 , microwave-assisted hydrothermal synthesis 31 , ultrasound synthesis 32 , etc. are popularly exercised. CDs can be synthesized either from inorganic materials 19,33 such as graphene 34 , carbon black 35 , and candle soot 12

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Paper strip based and live cell ultrasensitive lead sensor using carbon dots synthesized from biological media

Cited by 81 publications

References 39 publications

The quenching of the fluorescence of carbon dots: A review on mechanisms and applications

The quenching of the fluorescence of carbon dots: A review on mechanisms and applications

Labelling Proteins with Carbon Nanodots

DNA-damage and cell cycle arrest initiated anti-cancer potency of super tiny carbon dots on MCF7 cell line

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