Paraben concentrations found in human body fluids do not exert steroidogenic effects in human granulosa primary cell cultures

Herrera-Cogco, Elena; López-Bayghen, Bruno; Hernández-Melchor, Dinorah; López-Luna, Almena; Palafox-Gómez, Cecilia; Ramírez-Martínez, Leticia; López-Bello, Estheisy; Albores, Arnulfo; López-Bayghen, Esther

doi:10.1080/15376516.2020.1741052

Cited by 8 publications

(3 citation statements)

References 49 publications

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“…While not affecting oocyte viability, another study suggests that MP exposure to porcine oocytes inhibited expansion of the cumulus oocyte complex and final maturation of the oocyte (Barajas‐Salinas et al, 2021 ). In contrast, exposure to MP and BP did not alter the levels of E 2 , P 4 , or the mRNA levels of steroidogenic factors in human cumulus cells collected from women undergoing a standardized IVF protocol (Herrera‐Cogco et al, 2020 ).…”

Section: Parabensmentioning

confidence: 97%

The effects of endocrine‐disrupting chemicals on ovarian‐ and ovulation‐related fertility outcomes

Land

Miller

Fugate

et al. 2022

Molecular Reproduction Devel

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Exposure to endocrine‐disrupting chemicals (EDCs) is unavoidable, which represents a public health concern given the ability of EDCs to target the ovary. However, there is a large gap in the knowledge about the impact of EDCs on ovarian function, including the process of ovulation. Defects in ovulation are the leading cause of infertility in women, and EDC exposures are contributing to the prevalence of infertility. Thus, investigating the effects of EDCs on the ovary and ovulation is an emerging area for research and is the focus of this review. The effects of EDCs on gametogenesis, uterine function, embryonic development, and other aspects of fertility are not addressed to focus on ovarian‐ and ovulation‐related fertility issues. Herein, findings from epidemiological and basic science studies are summarized for several EDCs, including phthalates, bisphenols, per‐ and poly‐fluoroalkyl substances, flame retardants, parabens, and triclosan. Epidemiological literature suggests that exposure is associated with impaired fecundity and in vitro fertilization outcomes (decreased egg yield, pregnancies, and births), while basic science literature reports altered ovarian follicle and corpora lutea numbers, altered hormone levels, and impaired ovulatory processes. Future directions include identification of the mechanisms by which EDCs disrupt ovulation leading to infertility, especially in women.

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Section: Parabensmentioning

confidence: 97%

The effects of endocrine‐disrupting chemicals on ovarian‐ and ovulation‐related fertility outcomes

Land

Miller

Fugate

et al. 2022

Molecular Reproduction Devel

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show abstract

“…In the present study, our results demonstrate that most of the oocytes exposed to MePB remain arrested in GV, reducing MII rates. In addition, it was reported that human granulosa cells exposed to MePB and BuPB did not show changes in steroidogenesis (Herrera‐Cogco et al, 2020). Nevertheless, it is important to consider the differences between granulosa cells with steroidogenic capacity and cumulus cells, which do not possess this function.…”

Section: Discussionmentioning

confidence: 99%

Effects of methylparaben on in vitro maturation of porcine oocytes

Barajas-Salinas

Ducolomb

Betancourt

et al. 2020

J of Applied Toxicology

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Parabens (PBs) are compounds widely used in industry for food and personal care products as antimicrobials and preservatives. Their indiscriminate use has resulted in their detection in different ecosystems so that humans and other organisms are highly exposed. Methylparaben (MePB), compared with other PBs, is mostly detected in food, personal care and baby care products. PBs could be linked to the generation of hormonal disorders and fertility impairment since their recent classification as endocrine disruptors. The knowledge of the effects that MePB can exert is of great importance as, in terms of reproduction, information is limited. Therefore, the objective of the present study was to evaluate the effect of MePB on porcine oocyte viability and in vitro maturation (IVM), as well as to determine the lethal concentration at 50% (LC 50) and the maturation inhibition concentration at 50% (MIC 50). Oocytes were exposed to different MePB concentrations 0 (control), 50, 100, 500, 750 and 1000 μM during 44 h of IVM. Cytoplasmic alterations and reduced cumulus cell expansion were observed in oocytes exposed to MePB; however, viability was not affected. In addition, oocyte maturation decreased in a concentration-dependent manner after exposure to MePB. The estimated LC 50 was 2028.38 μM, whereas MIC 50 was 780.31 μM. To our knowledge, this is the first study that demonstrates that MePB altered porcine oocyte morphology, and caused a reduction in cumulus cell expansion, both of which resulted in decreased oocyte maturation. Therefore, MePB exposure may be one of the factors involved in fertility impairment in mammals, including that of humans.

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“…En el presente estudio se demostró que la mayoría de los ovocitos expuestos al MePB permanecen arrestados en VG, reduciendo las tasas de MII. En contraste, recientemente fue reportado que células de la granulosa humana expuestas a MePB y BuPB no afectan a los genes que regulan su esteroidogénesis, así como los niveles de progesterona ni la producción de estradiol(Herrera-Cogco, et al, 2020). Sin embargo, es importante considerar las diferencias entre las células de la granulosa con capacidad esteroidogénica y las CC, las cuales no presentan dicha característica.…”

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Efecto del metil-parabeno durante la maduración in vitro de ovocitos de cerdo

Salinas

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Paraben concentrations found in human body fluids do not exert steroidogenic effects in human granulosa primary cell cultures

Cited by 8 publications

References 49 publications

The effects of endocrine‐disrupting chemicals on ovarian‐ and ovulation‐related fertility outcomes

The effects of endocrine‐disrupting chemicals on ovarian‐ and ovulation‐related fertility outcomes

Effects of methylparaben on in vitro maturation of porcine oocytes

Efecto del metil-parabeno durante la maduración in vitro de ovocitos de cerdo

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