Paracoccus aeridis sp. nov., an indole-producing bacterium isolated from the rhizosphere of an orchid, Aerides maculosa

Abstract

A Gram-stain-negative, non-motile, coccoid-shaped, catalase- and oxidase-positive, non-denitrifying, neutrophilic bacterium designated as strain JC501T was isolated from an epiphytic rhizosphere of an orchid, Aerides maculosa, growing in the Western Ghats of India. Phylogenetic analyses based on the 16S rRNA gene sequence indicated that strain JC501T belonged to the genus Paracoccus and had the highest levels of sequence identity with Paracoccus marinus KKL-A5T (98.9 %), Paracoccus contaminans WPAn02T (97.3 %) and other members of the genus Paracoccus (<97.3 %). Strain JC501T produced indole-3 acetic acid and other indole derivatives from tryptophan. The dominant respiratory quinone was Q-10 and the major fatty acid was C18 : 1ω7c/C18 : 1ω6c, with significant quantities of C18 : 1ω9c, C17 : 0 and C16 : 0. The polar lipids of strain JC501T comprised phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, an unidentified glycolipid, two unidentified aminolipids, two unidentified lipids and four unidentified phospholipids. The genome of strain JC501T was 3.3 Mbp with G+C content of 69.4 mol%. For the resolution of the phylogenetic congruence of the novel strain, the phylogeny was also reconstructed with the sequences of eight housekeeping genes. Based on the results of phylogenetic analyses, low (<85.9 %) average nucleotide identity, digital DNA–DNA hybridization (<29.8 %), chemotaxonomic analysis and physiological properties, strain JC501T could not be classified into any of the recognized species of the genus Paracoccus . Strain JC501T represents a novel species, for which the name Paracoccus aeridis sp. nov. is proposed. The type strain is JC501T (=LMG 30532T=NBRC 113644T).

Paracoccus xiamenensis sp. nov., isolated from seawater on the Xiamen

“…At the time of writing, the genus Paracoccus contained 68 recognized species with validly published names (www.bacterio.net/paracoccus.html) [2]. Species of genus Paracoccus were isolated from various environments, including water [3–5], air [6], soil [7, 8], rhizosphere [9, 10], activated sludge [11, 12], sediment [13–15] and fish [16]. This study describes the taxonomic position of strain 12-3 T by a polyphasic approach.…”

Paracoccus amoyensis sp. nov., isolated from the surface seawater along the coast of Xiamen Island, China

“…xiamenensis 12-3 T (66.9 mol%) and P. pacificus F14 T (61.4 mol%), with their respective differences of 1.4, 6.2, 6.8 and 1.3 % [1–10, 10–18, 37]. Based on differences <1.0 % (cut-off value for defining a new species), strain 11-3 T can be considered as a new species [38].…”

“…At the time of writing, the genus Paracoccus included 73 validly published species (www.bacterio.net/paracoccus.html) [2]. Species of the genus Paracoccus comprise various bacteria with phylogenetic, phenotypic and metabolic diversity, and have been isolated from different environments, such as fish [3], activated sludge [4, 5], soil [6, 7], water [8–10], sediment [11–13], rhizosphere [14, 15] and air [16]. This study described a novel strain isolated from the surface seawater along the coast of Xiamen Island, China.…”

Paracoccus marinaquae sp. nov., isolated from coastal water of the Yellow Sea

“…In addition, bacteria of the genus Paracoccus were isolated from other various habitats, such as air (Xue et al 2017), soil (Sun et al 2015), sediment (Zhang et al 2019), seawater (Lyu et al 2021) and sediment of freshwater (Wang et al 2009). And they also exist in some plants (Rai et al 2020), animal species (Kim et al 2018; Lee et al 2019) and some extreme environments, such as hot spring (Ye et al 2020), plateau permafrost (Zhu et al 2013) and even oilcontaminated water (Zhang et al 2020b). Many Paracoccus spp.…”