Paradoxical Discrepancy Between the Serum Level and the Placental Intensity of PP5/TFPI-2 in Preeclampsia and/or Intrauterine Growth Restriction: Possible Interaction and Correlation with Glypican-3 Hold the Key

Platelets Contain Tissue Factor Pathway Inhibitor-2 Derived from Megakaryocytes and Inhibits Fibrinolysis

“…In agreement with recent observations (36,38), our data indicate that plasma levels of TFPI-2 in pregnancy during the onset of labor are ϳ7 nM. Based upon previous work with TFPI-1 (46, 47, 54 -56), we evaluated the binding of TFPI-2 to purified FV and FVa as well as to FV/Va in platelets and FV in pregnant woman plasma.…”

“…The plasma TFPI-2 levels in the third trimester of pregnancy range from the early reports of 1-3 nM (31)(32)(33)(34)(35) to up to 10 nM in recent reports (36,38). In one study, the mean plasma TFPI-2 level in pregnancy is reported to be 320 pM (31).…”

“…Interestingly, plasma TFPI-2 levels rise steadily during normal pregnancy and are highest at the onset of labor (31)(32)(33)(34)(35)(36). The plasma TFPI-2 levels in the third trimester of pregnancy range from the early reports of 1-3 nM (31)(32)(33)(34)(35) to up to 10 nM in recent reports (36,38).…”

“…Human plasma TFPI-2 levels are reported to be negligible (6,(31)(32)(33)(34)(35)(36); however, a mean value of 30 pM (range from 3 to 300 pM) in one study (37) and an average value of 0.5 nM (range not given) in an another study (38) have also been reported. Thus, plasma levels of TFPI-2 are below the limit of detection (Յ20 pM or 0.5 ng/ml) in the majority of the reports and are also observed to be low in the TFPI-2-transfected apoE-knock-out mice (39).…”

Silencing of tissue factor pathway inhibitor‐2 gene in malignant melanomas

“…21 Formalin-fixed and paraffin-embedded sections were sliced at 4 lm thickness, deparaffinized and heated in 10 mM citrate buffer (pH 6.0) for 20 min at 95°C. After blocking, the sections were incubated with the primary antibody at a dilution of 100-fold at 4°C for 1 hr, and then with the second anti- Region analyzed by the new set of primers, which is shown as ''MSP 2 in Figure 1a.''…”