Parallel expression profiling of barley–stem rust interactions

Zhang, Ling; Castell-Miller, Claudia V.; Dahl, Stephanie K.; Steffenson, Brian J.; Kleinhofs, A.

doi:10.1007/s10142-007-0069-0

Cited by 20 publications

(19 citation statements)

References 34 publications

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“…However, induction by fungal infection could not be established because of similar observations in mock and uninoculated controls. The variable Rpg5 mRNA levels were surprising, because previous experiments with Rpg1 showed low but steady mRNA levels with or without fungal infection (31,32).…”

Section: Discussionmentioning

confidence: 99%

The stem rust resistance gene Rpg5 encodes a protein with nucleotide-binding-site, leucine-rich, and protein kinase domains

Brueggeman

Druka²,

Nirmala

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

139

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We isolated the barley stem rust resistance genes Rpg5 and rpg4 by map-based cloning. These genes are colocalized on a 70-kb genomic region that was delimited by recombination. The Rpg5 gene consists of an unusual structure encoding three typical plant disease resistance protein domains: nucleotide-binding site, leucine-rich repeat, and serine threonine protein kinase. The predicted RPG5 protein has two putative transmembrane sites possibly involved in membrane binding. The gene is expressed at low but detectable levels. Posttranscriptional gene silencing using VIGS resulted in a compatible reaction with a normally incompatible stem rust pathogen. Allele sequencing also validated the candidate Rpg5 gene. Allele and recombinant sequencing suggested that the probable rpg4 gene encoded an actin depolymerizing factor-like protein. Involvement of actin depolymerizing factor genes in nonhost resistance has been documented, but discovery of their role in gene-for-gene interaction would be novel and needs to be further substantiated.actin depolymerizing factor ͉ barley ͉ disease resistance domains ͉ map-based cloning

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Section: Discussionmentioning

confidence: 99%

The stem rust resistance gene Rpg5 encodes a protein with nucleotide-binding-site, leucine-rich, and protein kinase domains

Brueggeman

Druka²,

Nirmala

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

139

View full text Add to dashboard Cite

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“…Bln2 was induced, however, upon challenge with Puccinia graminis f. sp. tritici, causal agent of stem rust (Zhang et al, 2008), whereas Bln1 was not. Conversely, it is possible that the observed noninduction of Bln1 in response to P. graminis f. sp.…”

Section: Bioinformatic Classification Of the Blufensin Family Of Smalmentioning

confidence: 95%

“…Barley1 GeneChip data files for the Fusarium graminearum (Boddu et al, 2006) and Puccinia graminis f. sp. tritici (Zhang et al, 2008) experiments are categorized under PLEXdb accession numbers BB9 and BB49, respectively.…”

Section: Microarray Data Accessmentioning

confidence: 99%

Blufensin1Negatively Impacts Basal Defense in Response to Barley Powdery Mildew

2008

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Plants have evolved complex regulatory mechanisms to control the defense response against microbial attack. Both temporal and spatial gene expression are tightly regulated in response to pathogen ingress, modulating both positive and negative control of defense. BLUFENSIN1 (BLN1), a small peptide belonging to a novel family of proteins in barley (Hordeum vulgare), is highly induced by attack from the obligate biotrophic fungus Blumeria graminis f. sp. hordei (Bgh), casual agent of powdery mildew disease. Computational interrogation of the Bln1 gene family determined that members reside solely in the BEP clade of the Poaceae family, specifically, barley, rice (Oryza sativa), and wheat (Triticum aestivum). Barley stripe mosaic virus-induced gene silencing of Bln1 enhanced plant resistance in compatible interactions, regardless of the presence or absence of functional Mla coiled-coil, nucleotide-binding site, Leu-rich repeat alleles, indicating that BLN1 can function in an R-gene-independent manner. Likewise, transient overexpression of Bln1 significantly increased accessibility toward virulent Bgh. Moreover, silencing in plants harboring the Mlo susceptibility factor decreased accessibility to Bgh, suggesting that BLN1 functions in parallel with or upstream of MLO to modulate penetration resistance. Collectively, these data suggest that the grass-specific Bln1 negatively impacts basal defense against Bgh.

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“…However, 11 genes were identified, which were exclusively up-regulated in the incompatible interaction, among them a leucine-rich repeat family protein, a calcineurin B-like protein-interacting protein kinase, a serine/threonine kinase, an ethylene-responsive RNA helicase and a protein-phosphatase 2C . Zhang et al (2008) analyzed the interaction of barley with the rust fungus Puccinia graminis f.sp. tritici (Pgt) by using the Barley1 GeneChip.…”

Section: Interactions With Biotrophic Fungimentioning

confidence: 99%

“…Dudler et al, 1991, Contigs on Barley1 annotated as GST genes (keyword search-based) were put into a gene list for creating a data output at PLEXdb/BarleyBase (Wise et al, 2007a). Data were exemplarily selected for ETItype powdery mildew resistance (experiment BB10, Meng et al, 2009), compatible interaction with F. graminearum and its trichothecene non-producing tri5 mutant (experiment BB52, Boddu et al, 2007) and ETI-type resistance and susceptibility to different races of the stem rust fungus (Pgt races MCC or QCC, experiment BB49, Zhang et al, 2008). Pictures show heatmaps of gene expression patterns normalized to an early time of a control experiment (yellow means up-regulation, blue means down-regulation).…”

Section: Enriched Biological Functionsmentioning

confidence: 99%