Parameter Optimization of Plant-based Decellularized Scaffold for Biomedical Applications Using Response Surface Methodology

Abstract

The banana leaf scaffold is used for response surface optimization because it is a plant-based scaffold; we have various scaffolds to perform this experiment. In this experiment, CCD-RSM has been used as an experimental design to increase the concentration of detergent (SDS), the concentration of CaCl2, the size of the banana leaf, rpm, and no. of days. Twenty-one experimental runs were performed, and porosity and swelling ratios were optimized. The optimum conditions for this process were 0.25, 0.05% w/v SDS, and CaCl2 (50mM, 150mM, 250mM), RPM (120,180,240), size of banana leaf (0.5,2.5cm), and period were used as optimizing parameters and later on, we calculated the optimizing characters like porosity and swelling ratio of the scaffolds thus formed. With the help of all this, we optimized the swelling ratio and porosity. Finally, by using the design expert, we could derive various tables and graphs, like contour and 3D, to better understand the results.