Paraquat Modulates Immunological Function in Bone Marrow-Derived Macrophages

Srinontong, Piyarat; Wandee, Jaroon; Angwanich, Worapol

doi:10.21203/rs.3.rs-407029/v1

Cited by 2 publications

(3 citation statements)

References 36 publications

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“…Effects are also in expose-time and PQconcentration dependent manners. The results further confirm our previous study that PQ increased the cytotoxicity of bone marrow-derived macrophages [14] and the previous report that PQ decreased human neural progenitor cells [15].…”

Section: Resultssupporting

confidence: 92%

“…Apoptosis constitutes one of the major mechanisms underlying pathological characterized caused by PQ [5]. We have previously shown that PQ caused apoptosis in macrophages [14]. To further elucidate the effects of ML extracts in PQ-induced apoptosis, thus cell apoptosis was subsequently performed.…”

Section: Effect Of Pq On Apoptosis Of Raw 2647 Macrophages After Pre-...mentioning

confidence: 99%

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Morus alba L. Leaf Extract Exerts Anti-Inflammatory Effect on Paraquat-Exposed Macrophages

et al. 2022

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Paraquat (PQ) is a widely used herbicide in agricultural areas. However, it has been reported to cause harm to animals or humans exposed to this herbicide. Generally, PQ stimulates the production of reactive oxygen species within cells causing cell death and resulting in bodily abnormalities. At present, efforts are being made to find drugs to reduce the toxic effects of PQ and natural products are targets of interest. This study aimed to assess the effect of mulberry (Morus alba L.) leaf extract on macrophages (RAW 264.7) after exposure to PQ, including the viability and expressions of NF-κB, IL-1β, and IL-6. We treated RAW 264.7 macrophages with Morus alba L. leaf extract in the absence or presence of PQ. Subsequently, viability, apoptosis, and expressions of NF-κB, IL-1β, and IL-6 were examined. It was found that the extract of mulberry leaves could inhibit apoptosis, increase viability, and significantly downregulates expressions of NF-κB, IL-1β, and IL-6 when RAW 264.7 macrophages were exposed to PQ. These phenomena indicated that Morus alba L. leaf extract has properties to reduce the inflammatory effect caused by the herbicide PQ. HIGHLIGHTS Morus alba L. leaf extract improved the PQ-induced apoptosis of RAW 264.7 macrophage Morus alba L. leaf extract inhibited the expression of NF-κB, IL-1β, and IL-6 of RAW 264.7 macrophages GRAPHICAL ABSTRACT

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Section: Resultssupporting

confidence: 92%

Section: Effect Of Pq On Apoptosis Of Raw 2647 Macrophages After Pre-...mentioning

confidence: 99%

Morus alba L. Leaf Extract Exerts Anti-Inflammatory Effect on Paraquat-Exposed Macrophages

et al. 2022

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“…MDA levels in the cell culture supernatants were determined after 48 h of LPS exposure using a thiobarbituric acid reactive substance assay described by Srinontong et al . [ 21 ]. Briefly, aliquots of the reaction mixture containing cell supernatant, 0.12 M thiobarbituric acid, 0.9% NaCl, and 10% trichloroacetic acid were heated at 100°C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

Comparison of lipopolysaccharide-mediated peripheral blood mononuclear cell activation between Brahman and Brahman x Thai native crossbreed cattle

Srinontong,

Aengwanich,

Somphon

et al. 2024

Vet World

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Background and Aim: Lipopolysaccharide (LPS) is a robust endotoxin known to activate the immune system in cattle. The objective of this study was to investigate the effect of LPS on the morphology, cell viability, malondialdehyde (MDA), nitric oxide (NO), and total antioxidant capacity (TAC) of peripheral blood mononuclear cells (PBMCs) in Brahman and Brahman x Thai native crossbreed cattle. Materials and Methods: PBMCs were isolated from Brahman and Brahman x Thai native crossbreed cattle and treated with 0, 0.1, 1, and 10 μg/mL Escherichia coli LPS, respectively. Morphological changes in PBMCs were assessed at 24 and 48 h. In addition, we measured PBMC cell viability, MDA, NO, and TAC. Results: LPS stimulation caused cell deformation and partial PBMC area enlargement, but there were no differences between Brahman and Brahman x Thai native crossbreed cattle. Stimulation at all levels did not affect the viability of PBMCs (p > 0.05). MDA and NO levels were significantly higher in Brahman cattle than in Brahman Thai native crossbred cattle (p < 0.05). TAC was significantly higher in Brahman x Thai native crossbred cattle than in Brahman cattle (p < 0.05). Conclusion: Immune cells of crossbreed cattle have a higher activation response to LPS than those of purebred cattle, and native crossbreed beef cattle have a higher antioxidant capacity than purebred beef cattle. This result may explain why hybrid cattle of indigenous breeds are more resistant to disease than purebred cattle. Keywords: Brahman cattle, lipopolysaccharide, oxidative stress, peripheral blood mononuclear cells, Thai native crossbreed cattle.

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Paraquat Modulates Immunological Function in Bone Marrow-Derived Macrophages

Cited by 2 publications

References 36 publications

Morus alba L. Leaf Extract Exerts Anti-Inflammatory Effect on Paraquat-Exposed Macrophages

Morus alba L. Leaf Extract Exerts Anti-Inflammatory Effect on Paraquat-Exposed Macrophages

Comparison of lipopolysaccharide-mediated peripheral blood mononuclear cell activation between Brahman and Brahman x Thai native crossbreed cattle

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