Parkia pendula lectin as histochemistry marker for meningothelial tumour

Beltrão, Eduardo Isidoro Carneiro; Medeiros, Paloma Lys de; Rodrigues, Onaldo Guedes; Figuerêdo-Silva, José; Valênça, Marcelo Moraes; Coelho, Luana Cassandra Breitenbach Barroso; Carvalho, Luiz Bezerra de

doi:10.4081/819

Cited by 26 publications

(20 citation statements)

References 21 publications

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“…Lectin histochemistry has been frequently used to visualize the morphology of vascular networks in a variety of organs, either on tissue sections (Porter et al, 1990;Hansen-Smith et al, 1988;Nanka et al, 2001;Beltrão et al, 2003;Jilani et al, 2003;Cebasek et al, 2004) or using intravascular perfusion (Thurston et al, 1998Murphy et al, 1999;Debbage et al, 2001;Jilani et al, 2003). However, lectins have been only rarely used to delineate the vascular tree in the central nervous system (Porter et al, 1990), although their affinity to brain endothelium has been extensively characterized (Nag 1985a, b;Vorbrodt, 1986;Vorbrodt et al, 1986;Alroy et al, 1987;Fatehi et al, 1987;Porter et al, 1990;Debbage et al, 1996;Gerhart et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Lycopersicon esculentum lectin: an effective and versatile endothelial marker of normal and tumoral blood vessels in the central nervous system

Mazzetti¹,

Frigerio²,

Gelati³

et al. 2009

Eur J Histochem

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The binding of Lycopersicon esculentum lectin (LEA) to the vascular endothelium was studied in the central nervous system of rat, mouse and guinea pig at different developmental ages, and in a gliosarcoma model. Our observations showed that LEA consistently stained the entire vascular tree in the spinal cord and in the brain of all animal species at all developmental ages investigated. In the tumor model, the staining of the vascular network was very reproducible, enabled an easy identification of vascular profiles and displayed a higher efficiency when compared to two other commonly used vascular marker (EHS laminin and PECAM-1). Moreover, our results showed that LEA staining was comparable in both vibratome and paraffin sections and could be easily combined with other markers in double labeling experiments. These observations indicate that LEA staining may represent an effective and versatile endothelial marker for the study of the vasculature of the central nervous system in different animal species and experimental conditions.

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Section: Discussionmentioning

confidence: 99%

Lycopersicon esculentum lectin: an effective and versatile endothelial marker of normal and tumoral blood vessels in the central nervous system

Mazzetti¹,

Frigerio²,

Gelati³

et al. 2009

Eur J Histochem

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“…Several methods regarding the use of lectins as tools for recognizing aberrant glycans have been proposed in recent days [30,31,32]. The technique most common and widespread is the use of lectins in immunohistochemical assays.…”

Section: Application Of Plant Lectins In the Diagnosis Of Malignant Tmentioning

confidence: 99%

Biological Applications of Plants and Algae Lectins: An Overview

Teixeira¹,

Arruda²,

Nascimento³

et al. 2012

Carbohydrates - Comprehensive Studies on Glycobiology and Glycotechnology

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“…Protocols were carried out as described by Beltrão et al (21). Horseradish peroxidase (HRP)-conjugated WGA, PNA and Con A lectin were used to investigate the saccharide profile of the hepatic and intestinal schistosome egggranuloma system after treatment of S. mansoni-infected mice with free and encapsulated sulfated glucan.…”

Section: Lectin Histochemistry Of the Egg-granuloma Systemmentioning

confidence: 99%

“…In view of their capacity to recognize carbohydrates, lectins have been used as histochemical markers for cell outer-membrane glycoconjugates, especially for detecting changes that the latter undergo in malignancy (21)(22)(23), in inflammatory processes (23)(24)(25) and in parasite-host interactions (26,27).…”

mentioning

confidence: 99%