Parthenolide promotes apoptotic cell death and inhibits the migration and invasion of SW620 cells

Abstract

Background/AimsParthenolide (PT), a principle component derived from feverfew (Tanacetum parthenium), is a promising anticancer agent and has been shown to promote apoptotic cell death in various cancer cells. In this study, we focused on its functional role in apoptosis, migration, and invasion of human colorectal cancer (CRC) cells.MethodsSW620 cells were employed as representative human CRC cells. We performed the MTT assay and cell cycle analysis to measure apoptotic cell death. The wound healing, Transwell migration, and Matrigel invasion assays were performed to investigate the effect of PT on cell migration/invasion. Western blotting was used to establish the signaling pathway of apoptosis and cell migration/invasion.ResultsPT exerts antiproliferative effect and induces apoptotic cell death of SW620 cells. In addition, PT prevents cell migration and invasion in a dose-dependent manner. Moreover, PT markedly suppressed migration/invasion-related protein expression, including E-cadherin, β-catenin, vimentin, Snail, cyclooxygenase-2, matrix metalloproteinase-2 (MMP-2), and MMP-9 in SW620 cells. PT also inhibited the expression of antiapoptotic proteins (Bcl-2 and Bcl-xL) and activated apoptosis terminal factor (caspase-3) in a dose-dependent manner.ConclusionsOur results suggest that PT is a potential novel therapeutic agent for aggressive CRC treatment.

ACT001 can prevent and reverse tamoxifen resistance in human breast cancer cell lines by inhibiting NF‐κB activation

“…Consistently, we found that tamoxifen induced migration at low concentrations. PTL was also shown to suppress cell migration and invasion in human cancer cells, and could inhibit focal adhesion kinase‐mediated cell invasion . Similarly, we found that the migration induced by tamoxifen can be inhibited by ACT001.…”

Collateral Sensitivity of Parthenolide via NF-κB and HIF-α Inhibition and Epigenetic Changes in Drug-Resistant Cancer Cell Lines

“…The cytotoxicity of PT found in our analysis has been confirmed by other investigations. PT caused cells death in SW620 cells by inhibiting migration/invasion proteins such as E-cadherin, β-catenin, vimentin, Snail, cyclooxygenase-2, matrix metalloproteinase-2 (MMP-2), and MMP-9 as well as by activating caspase-3 (Liu et al, 2017). It also showed growth inhibitory effects of human cervical cancer (SiHa), breast cancer (MCF-7) cell lines (Al-Fatlawi et al, 2015), non-small cell lung cancer (GLC-82) (Lin et al, 2017), immortalized keratinocytes HaCaT and melanoma cells A375 (George et al, 2016).…”

Redox Paradox: A Novel Approach to Therapeutics-Resistant Cancer

“…Parthenolide (PN) is a sesquiterpene lactone that is found in flowers and fruits of the traditional herbal medicine fever-few (Tanacetum parthenium). PN acts as a pro-oxidant/ antioxidant that can elicit a protective response in normal cells but adds injury to tumor cells after radiation therapy (197,202,364). For instance, PN induced apoptosis in the AML-French-American-British subtype cancer while sparing normal hematopoietic cells (CD34 + /CD38 -) and progenitor cells (262).…”