Partial characterization and activities of proteases from the digestive tract of discus fish (Symphysodon aequifasciata)

Shu‐Chien, Alexander Chong; Hashim, Roshada; Lee, Chow‐Yang; Ali, Ahmad

doi:10.1016/s0044-8486(01)00630-5

Cited by 157 publications

(137 citation statements)

References 31 publications

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“…This is because the gut microflora in fishes and crustaceans can metabolize several nutrients that the host cannot and can convert them to end products that are beneficial to the host (Suzer et al, 2007;Hoyoux et al, 2009). It has been reported that intestinal microorganisms have a beneficial effect on the digestive process of fish such as in the microbial breakdown of cellulose (Saha and Ray, 1998;Bairagi et al, 2002;Saha et al, 2006;Mondal et al, 2008;Ray et al, 2010), starch (Sugita et al, 1997), protein (Chong et al, 2002;Fu et al, 2005;Silvia et al, 2006) and lipid (Tanu et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Activities of Intestinal Bacteria Isolated from Farmed <i>Clarias gariepinus</i>

Ariole¹,

Nwogu²,

Chuku³

2014

ija

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The culturable bacteria associated with the digestive tract of a freshwater cultured fish, Clarias gariepinus, and their degradative abilities were established. The spread plate method was employed for bacterial isolation. The bacterial isolates were qualitatively screened for extracellular enzyme-producing ability using milk agar, starch agar, egg yolk agar and cellulose agar for protease, amylase, lipase and cellulase activities respectively. A total of 18 bacterial isolates were identified. Bacteria of the genera Bacillus, Staphylococcus, Vibrio, Aeromonas, Pseudomonas, Lactobacillus, Escherichia, Salmonella, Enterobacter, Micrococcus and Flavobacterium were isolated from fish digestive tract at different frequencies with Bacillus predominating. Enzymatic studies indicated that the bacterial isolates possess the ability to degrade proteins, starch, lipids and cellulose. The percentage composition of enzyme-producing bacteria are -protease producing strains (72.2%), lipase producing strains (61.1%), amylase producing strains (55.6%) and cellulose producing strains (38.9%). All the isolates possessed multienzyme activity. An isolate (Bacillus sp. B 1 ) showed activity for protease, amylase, lipase and cellulase enzymes. Therefore, the isolated indigenous multiple enzyme-producing strains can be effectively exploited for use as probiotics while formulating aquafeeds.

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Section: Introductionmentioning

confidence: 99%

Enzymatic Activities of Intestinal Bacteria Isolated from Farmed <i>Clarias gariepinus</i>

Ariole¹,

Nwogu²,

Chuku³

2014

ija

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“…The peptidase activities were generally higher in the intestine than in the stomach of fishes, which was an expected result since both extracellular and intracellular activities could contribute to protein digestion in alkaline milieu. Chong et al (2002) showed that either chymotrypsin-like or trypsin-like activities were higher in the intestine than in the stomach of the juvenile carnivorous fish, Symphysodon aequifasciata. Lundstedt et al (2004) found high peptidase activity in the stomachs of spotted sorubim Pseudoplatystoma corruscans.…”

Section: Discussionmentioning

confidence: 99%

Enzymatic Activity in the Gastrointestinal Tract of Pimelodus maculatus (Teleostei, Siluriformes) in Two Neotropical Reservoirs with Different Trophic Conditions

Duarte

Bemquerer

Araújo

2015

Braz. arch. biol. technol.

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Enzymatic activities for digestion of proteins and carbohydrates were compared among three organs of the digestive system of Pimelodus maculatus in two reservoirs with different trophic conditions during the winter of 2006. The aim was to test the hypothesis that enzymatic activity for the digestion of proteins and carbohydrates differed among organs and that such activities differ between the trophic state of the environment. Enzymatic activities were determined through the assays of specificity for trypsin, chymotrypsin and β-glucosidase enzymes. The intestine had higher trypsin-like enzymatic activities compared to the stomach and liver. The highest β-glucosidase activity was found in the liver compared to the stomach and intestine in the oligotrophic reservoir only. Overall, enzymatic activity did not differ between the eutrophic and oligotrophic reservoirs, although the intestinal chymotrypsin was comparatively higher in the eutrophic reservoir and the hepatic β-glucosidase was higher in the oligotrophicreservoir. These findings indicated that most digestive activity occurred in the intestine for P. maculatus, which was probably related to its omnivorous/carnivorous feeding habits. The highest proteolytic activity in the intestine was expected for most fishes, but the high hepatic β-glucosidase in the oligotrophic reservoir was unexpected. The hepatic β-glucosidase as well as the intestinal chymotrypsin-like activity could be considered as the candidates for biomarkers of environmental quality.

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“…The method adopted was described by Chong et al (2002) with little modification. Protease activity was assayed using 0.6% casein solution in 0.05 M Tris buffer solution (pH 6.0-9.0) at 37°C.…”

Section: Determination Of Optimum Phmentioning

confidence: 99%

“…Inhibitory assay: The method used was described by Chong et al (2002) with a slight modification. Briefly, 0.1 mL of the crude protease extract and 0.1 mL of 3.5% v/v of the volatile oil (as inhibitor) in 0.5% v/v Tween 80 solutions were added concomitantly to different concentration of casein solution (0.2-1.0% w/v) in 0.05 M Tris buffer, pH 8.0.…”

Section: Determination Of Optimum Phmentioning

confidence: 99%

Antimicrobial Assay for the Volatile Oils of Chromolaena odorata and its Inhibition against the Partially Purified and Characterized Extracellular Protease of Pseudomonas aeruginosa

Adeola¹,

Folorunso²,

Raimi³

et al. 2015

Asian J. of Biochemistry

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The recent development in the antimicrobial therapy to meet the challenges of resistant pathogenic bacteria has called for rapid growth in exploring natural products as alternative. The antimicrobial activity of the oils from the leaf and stem of Chromolaena odorata against nine enteric pathogenic bacteria was assessed and compared with commonly used antibiotics. Specifically, we examined the mode of inhibition of these oils on the extracellular protease of Pseudomonas aeruginosa. The inhibition zones of the oils range from 13.0±1.0-43.5±2.5 mm in Salmonella paratyphimurium and Shigella dysenteriae, respectively. Ceftriaxone has the highest inhibition of 26.0±2.0 mm against Salmonella paratyphimurium. There was a significant difference (p<0.05) between the average inhibition of the antibiotics, 5.0±0.82 mm and the volatile oils, 18.0±4.0 mm. Each of the microbes was sensitive to at least one of the oils. The extracellular protease of Pseudomonas aeruginosa had optimal activities at pH 7. protein) was achieved from the crude enzyme with DEAE anion exchange chromatography. The chromatographic peaks may be an evidence of multiple subunits of this enzyme. Therefore, the oil of Chromolaena odorata possessed antimicrobial activity and its ability to inhibit extracellular protease of Pseudomonas aeruginosa will be a possible source of nutraceuticals for clinical purpose.

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Partial characterization and activities of proteases from the digestive tract of discus fish (Symphysodon aequifasciata)

Cited by 157 publications

References 31 publications

Enzymatic Activities of Intestinal Bacteria Isolated from Farmed <i>Clarias gariepinus</i>

Enzymatic Activities of Intestinal Bacteria Isolated from Farmed <i>Clarias gariepinus</i>

Enzymatic Activity in the Gastrointestinal Tract of Pimelodus maculatus (Teleostei, Siluriformes) in Two Neotropical Reservoirs with Different Trophic Conditions

Antimicrobial Assay for the Volatile Oils of Chromolaena odorata and its Inhibition against the Partially Purified and Characterized Extracellular Protease of Pseudomonas aeruginosa

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