Partial characterization of an extracellular polysaccharide produced by the moderately halophilic bacterium<i>Halomonas xianhensis</i>SUR308

Biswas, Jhuma; Ganguly, Jhuma; Paul, A. K.

doi:10.1080/08927014.2015.1106479

Cited by 25 publications

(20 citation statements)

References 52 publications

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“…along with respective standards (Ledezma et al 2016). Moreover, the FT-IR spectrum showed the presence of polysaccharides in the purified EPS with the full stretch appeared between 1,000 -1,300 (Biswas et al 2015). It has been reported that polysaccharides are the major components of EPS (Vu et al 2009) which include homopolysaccharides (α-D-glucan, β-D-glucans, fructans and polygalactan) and heteropolysaccharides (D-glucose, D-galactose, L-rhamnose, N-acetylglucosamine and glucuronic acid) (Ruas-Madiedo et al 2002).…”

Section: Discussionmentioning

confidence: 95%

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Antifouling activities of extracellular polymeric substances produced by marine bacteria associated with the gastropod (Babylonia sp.)

Viju¹,

Ezhilraj²,

Shankar³

et al. 2018

Nova Biotechnologica Et Chimica

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Bacteria associated with surfaces have been frequently cited as a potential source for the isolation of bioactive metabolites. In this study, bacteria associated with marine gastropod, Babylonia sp. were isolated and screened for antibacterial activity against biofilm-forming bacteria. The antibiofilm and antifouling effect of the selected surface- associated bacterial strains were examined under in vitro and in vivo conditions. Results showed that the extracellular polymeric substances (EPS) of the bacterial strain CML associated with gastropod species considerably reduced the adhesion of biofilm-forming bacteria on glass coupons. Besides, the antifouling coat prepared by incorporating of this EPS into polyurethane varnish prevented the settlement of biofoulers on test substratum submerged in marine waters. The functional groups present in the EPS were analyzed using FT-IR. The bacterium responsible for the production of the bioactive EPS was identified as Bacillus subtilis subsp. by 16S rRNA gene sequencing. More detailed characterization of the identified bioactive EPS could lead to the isolation of a novel natural antifouling product.

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Section: Discussionmentioning

confidence: 95%

“…7). It has been reported that the broad peak appeared between 3,600 -3,200 could indicate the presence of saccharides (Iyer et al 2005) while the peaks appearing between 1,000 -1,300 could be due to the presence of polysaccharides (Biswas et al 2015).…”

Section: Fourier Transform Infrared (Ft-ir) Analysismentioning

confidence: 99%

Antifouling activities of extracellular polymeric substances produced by marine bacteria associated with the gastropod (Babylonia sp.)

Viju¹,

Ezhilraj²,

Shankar³

et al. 2018

Nova Biotechnologica Et Chimica

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“…Production and characterization of exopolysaccharide of H. xianhensis SUR308 (GenBank Accession No. KJ933394) has been reported recently [12],[13]. The present study was conducted to optimize the medium components and other environmental parameters for EPS production by H. xianhensis SUR308 under batch cultivation.…”

Section: Introductionmentioning

confidence: 92%

Optimization of factors influencing exopolysaccharide production by <em>Halomonas xianhensis</em> SUR308 under batch culture

Biswas

Paul

2017

AIMS Microbiology

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A moderately halophilic bacterium, Halomonas xianhensis SUR308 (GenBank Accession No. KJ933394) was isolated from multi-pond solar salterns of Odisha, India. Exopolysaccharide (EPS) production by this strain in malt extract yeast extract (MY) medium has been optimized under batch culture system. Among the different media tested, MY medium showed an EPS production of 2.55 g/L, which increased to 2.85 g/L under optimized aeration. An initial pH of 7.5 and incubation temperature of 32 °C were found to be most suitable for EPS production by the isolate under aerobic condition. An EPS production of 3.85 g/L was achieved when the growth medium was supplemented with 2.5% NaCl. Glucose was the most favourable carbon source for EPS production and maximum production (5.70 g/L) was recorded with 3% glucose. However, growth as well as production of EPS was remarkably affected when the growth medium was supplemented with hydrocarbons as sole source of carbon. Among different nitrogen sources, casein hydrolysate at 0.5% level was proved to be the best for EPS production and an initial inoculum dose of 7% (v/v) enhanced the EPS production to 7.78 g/L, while the divalent metal ions were in general toxic to growth and EPS production, EPS synthesis by SUR308 was enhanced with Cr (VI) supplementation.

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“…While the carbohydrate content was quantified according to Dubois et al [22], Folin's phenol method [24], and the method of Filisetti-Cozzi and Carpita [25] were followed for determination of protein and uronic acid, respectively. Details of the methodologies adopted for paper chromatography, gas chromatography (GC), and gas chromatographic-mass spectrometry (GC-MS) analysis were the same as those described in Biswas et al [23].…”

Section: Compositional Analysismentioning

confidence: 99%

Production and characterization of a bioactive extracellular homopolysaccharide produced by Haloferax sp. BKW301

et al. 2020

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The production of extracellular polysaccharides (EPS) by haloarchaeal members, with novel and unusual physicochemical properties, is of special importance and has the potential for extensive biotechnological exploitation. An extremely halophilic archaeon, Haloferax sp. BKW301 (GenBank Accession No. KT240044) isolated from a solar saltern of Baksal, West Bengal, India has been optimized for the production of EPS under batch culture. It produced a considerable amount (5.95 g/L) of EPS in the medium for halophiles with 15% NaCl, 3% glucose, 0.5% yeast extract, and 6% inoculum under shake flask culture at 120 rpm. The purified EPS, a homopolymer of galactose as revealed by chromatographic methods and Fourier‐transform infrared spectroscopy, is noncrystalline (CIxrd, 0.82), amorphous, and could emulsify hydrocarbons like kerosene, petrol, xylene, and so forth. Moreover, the polymer is highly thermostable (up to 420°C) and displayed pseudoplastic rheology. Biologically, the EPS was able to scavenge DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) radical efficiently and inhibit the proliferation of the Huh‐7 cell line at an IC50 value of 6.25 µg/ml with a Hill coefficient of 0.844. Large‐scale production of this thermostable, pseudoplastic homopolysaccharide, therefore, could find suitable applications in industry and biotechnology.

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Partial characterization of an extracellular polysaccharide produced by the moderately halophilic bacteriumHalomonas xianhensisSUR308

Cited by 25 publications

References 52 publications

Antifouling activities of extracellular polymeric substances produced by marine bacteria associated with the gastropod (Babylonia sp.)

Antifouling activities of extracellular polymeric substances produced by marine bacteria associated with the gastropod (Babylonia sp.)

Optimization of factors influencing exopolysaccharide production by <em>Halomonas xianhensis</em> SUR308 under batch culture

Production and characterization of a bioactive extracellular homopolysaccharide produced by Haloferax sp. BKW301

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