Partial denitrification providing nitrite: Opportunities of extending application for anammox

Du, Rui; Peng, Yongzhen; Ji, Jiantao; Shi, Lei; Gao, Ruitao; Li, Xiangchen

doi:10.1016/j.envint.2019.105001

Cited by 300 publications

(108 citation statements)

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“…Notably, nitrate reductase, which facilitates the conversion of nitrate to nitrite, was the most common nitrogen marker gene in the PNA system, with a presence in more than half of the hybrid-assembled MAGs ( n = 29), and contributes to the previously proposed nitrite loop [ 13 , 74 ]. Although no cytochrome cd1 nitrite reductase ( nir S) or copper-containing nitrite reductase ( nir K) enzymes could be found in the genome of the predominant anammox bacteria (H1_AMX1), another gene that annotated as hao in H1_AMX1 had a high similarity (83% amino acid similarity) with kustc 0458, a likely candidate to reduce nitrite to nitric oxide [ 75 ].…”

Section: Resultsmentioning

confidence: 99%

“…Although no cytochrome cd1 nitrite reductase ( nir S) or copper-containing nitrite reductase ( nir K) enzymes could be found in the genome of the predominant anammox bacteria (H1_AMX1), another gene that annotated as hao in H1_AMX1 had a high similarity (83% amino acid similarity) with kustc 0458, a likely candidate to reduce nitrite to nitric oxide [ 75 ]. Additionally, the widespread presence of nitrite reductase ( nir K or nir S gene were identified in 20 MAGs) may contribute to substrate (nitric oxide, NO) generation for the anammox process, while also providing detoxification and improving the nitrogen removal efficiency in the system [ 13 , 74 ]. However, the exchangeable intermediates or cross-feeding in the microbial community should be explored and validated by using other technologies, e.g., isotope-pairing experiments [ 76 ].…”

Section: Resultsmentioning

confidence: 99%

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High-quality bacterial genomes of a partial-nitritation/anammox system by an iterative hybrid assembly method

et al. 2020

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Background Genome-centric approaches are widely used to investigate microbial compositions, dynamics, ecology, and interactions within various environmental systems. Hundreds or even thousands of genomes could be retrieved in a single study contributed by the cost-effective short-read sequencing and developed assembly/binning pipelines. However, conventional binning methods usually yield highly fragmented draft genomes that limit our ability to comprehensively understand these microbial communities. Thus, to leverage advantage of both the long and short reads to retrieve more complete genomes from environmental samples is a must-do task to move this direction forward. Results Here, we used an iterative hybrid assembly (IHA) approach to reconstruct 49 metagenome-assembled genomes (MAGs), including 27 high-quality (HQ) and high-contiguity (HC) genomes with contig number ≤ 5, eight of which were circular finished genomes from a partial-nitritation anammox (PNA) reactor. These 49 recovered MAGs (43 MAGs encoding full-length rRNA, average N50 of 2.2 Mbp), represented the majority (92.3%) of the bacterial community. Moreover, the workflow retrieved HQ and HC MAGs even with an extremely low coverage (relative abundance < 0.1%). Among them, 34 MAGs could not be assigned to the genus level, indicating the novelty of the genomes retrieved using the IHA method proposed in this study. Comparative analysis of HQ MAG pairs reconstructed using two methods, i.e., hybrid and short reads only, revealed that identical genes in the MAG pairs represented 87.5% and 95.5% of the total gene inventory of hybrid and short reads only assembled MAGs, respectively. In addition, the first finished anammox genome of the genus Ca. Brocadia reconstructed revealed that there were two identical hydrazine synthase (hzs) genes, providing the exact gene copy number of this crucial phylomarker of anammox at the genome level. Conclusions Our results showcased the high-quality and high-contiguity genome retrieval performance and demonstrated the feasibility of complete genome reconstruction using the IHA workflow from the enrichment system. These (near-) complete genomes provided a high resolution of the microbial community, which might help to understand the bacterial repertoire of anammox-associated systems. Combined with other validation experiments, the workflow can enable a detailed view of the anammox or other similar enrichment systems.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

High-quality bacterial genomes of a partial-nitritation/anammox system by an iterative hybrid assembly method

et al. 2020

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“…Moreover, a single-reactor (i.e., upflow anaerobic sludge blanket, UASB) for simultaneous PDA was also developed, a 225-days operation demonstrated that the relatively low total nitrogen (TN) effluent concentration of 6.56 mg/L achieved even in a cold temperature (17.5°C). These experimental results provided a new insight on the nitrogen removal when combined with economical and sustainable anammox process, thus gave a summary and discussion on the current status of researches and future perspectives of PDA seemed necessary [8]. Our view was shifted to this promising coupling process and considerable novel outcomes were noticed, including influencing factors, principled characteristics, and economical analyses to elucidate this significant cost-effective method.…”

Section: Introductionmentioning

confidence: 89%

“…--N in raw wastewater, which was critical factor for terminating NO 3 --N reduction only to NO 2 --N. The ratio of COD/NO 3 --N largely determined the denitrification process and controlled the reduction rate of NO 3 --N [26]. Widely reported appropriate COD/NO 3 --N ratio for PD process ranged from 2.0~3.5, of which high NTR performance and anammox bacteria suitable influent substrate (NH 4 + -N/NO 2 --N=1.30) could be available [8,22,31]. However, chemoautotrophic anammox bacteria directly converted NH 4 + -N and NO 2 --N into nitrogen gas without carbon source addition and organics played a negative role in this process [32].…”

Section: Cod/no 3 --N Limited Cod/no 3 --N Means Low Level Cod or Sufmentioning

confidence: 99%

“…Recently, the high NO 2 --N producing from NO 3 --N reduction was observed in partial denitrification (PD, NO 3 --N → NO 2 --N) process and over 80% nitrate-to-nitrite transformation ratio (NTR) was obtained [6,7], indicating a more stable alternative to providing substrate for anammox bacteria compared with current extensively studied partial nitritation technology [8]. Cao et al firstly enriched NO 2 --N accumulation sludge using sludge fermentation liquid as external organics and a robust high nitrite of 20.91 ± 0.52 mg/L was obtained in a 108-day operation [6].…”

Section: Introductionmentioning

confidence: 99%

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New Insight into Partial Denitrification (PD)-based Anammox Process and Potential Engineering Application: A Review

Bahtiar¹,

Zhang²,

Wang³

et al. 2020

JHER

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Partial denitrification in domestic sewage and nitrate wastewater: study of the initiation, influencing factors and operational performance

Ma,

Qin,

Chen

et al. 2023

J of Chemical Tech & Biotech

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BackgroundPractical application of the anaerobic ammonium oxidation (anammox) process has been limited by the difficulty of obtaining a stable supply of NO2‐‐N. In this study, partial denitrification was utilized as a novel approach to obtain nitrite for anammox, investigating the initiation, influencing factors and performance of partial denitrification in the simultaneous treatment of domestic sewage and nitrate wastewater.ResultsWhen the influent concentration of NO3‐‐N was 40 mg/L and the chemical oxygen demand to nitrate (COD/NO3‐‐N) ratio was 2.7, the NO2‐‐N accumulation rate exceeded 80%, with an effluent NO2‐‐N concentration of 35.64 mg/L. Furthermore, the partial denitrification performance was good. When the volume ratio of domestic sewage and nitrate wastewater was adjusted to 2:3 and sodium acetate was added to form a COD/NO3‐‐N ratio of 3.6, the effluent NO2‐‐N/NH4+‐N ratio was 1.24 and the effluent NO2‐‐N concentration and average NO2‐‐N accumulation rate reached >31.24 mg/L and 83.38%, respectively, effectively ensuring influent requirements for anammox.ConclusionThe NO3‐‐N reduction rate , NO3‐‐N removal rate and NO2‐‐N accumulation rate were positively correlated with the influent nitrate concentration when ranging between 15‐40 mg/L. An appropriate increase in the influent COD/NO3‐‐N ratio was conducive to a reduction in the time required for maximum levels of NO2‐‐N accumulation to occur, with an influent NO3‐‐N concentration of 40 mg/L. The partial denitrification system could simultaneously treat domestic sewage and nitrate wastewater, effectively ensuring that the generated effluent met the influent requirements for subsequent anammox.This article is protected by copyright. All rights reserved.

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Partial denitrification providing nitrite: Opportunities of extending application for anammox

Cited by 300 publications

References 100 publications

High-quality bacterial genomes of a partial-nitritation/anammox system by an iterative hybrid assembly method

High-quality bacterial genomes of a partial-nitritation/anammox system by an iterative hybrid assembly method

New Insight into Partial Denitrification (PD)-based Anammox Process and Potential Engineering Application: A Review

Partial denitrification in domestic sewage and nitrate wastewater: study of the initiation, influencing factors and operational performance

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