Partial Purification and Antimycobacterial Screening of the Ethyl Acetate Extract of Alcaligenes faecalis BW1

In vitro antibacterial activities and molecular characterization of bacterial species isolated from farmlands against selected pathogens

“…According to [ 6 , 47 , 66 , [68] , [69] , [70] ] the antibacterial activities displayed by the antagonizing bacteria are as a result of production of antibacterial agents Surfactin and bacteriocin which include mersacidin and erisin are some of the antibacterial compounds produced by Bacillus species responsible for their antibacterial activities [ 23 , [71] , [72] , [73] , [74] ]. Brochocin-C is a bacteriocin produced by Brochothrix species, known for its broad spectrum activity [ 68 ].…”

A Metagenomic Nanopore Sequence Analysis Combined with Conventional Screening and Spectroscopic Methods for Deciphering the Antimicrobial Metabolites Produced by Alcaligenes faecalis Soil Isolate MZ921504

“…Soil significantly have been associated with several microorganisms that are antimicrobial producers and have been used extensively for antibiotic production, such as streptomycin from Streptomyces species [ 22 ]. A. faecalis has not always been associated with antimicrobial production, scarce studies on antimicrobial activity of A. faecalis have been published in the last few years [ 17 , 18 , 19 ]. In this study, it shows a promising approach for new antibiotic discovery as per secondary metabolite gene cluster results [ 17 ] and correlating these results with the antimicrobial and LC/MS spectroscopic analysis.…”

“…and reference organisms including, E. coli , Bacillus subtilis and Shigella flexneri , but showed no inhibition against Salmonella paratyphi and S. aureus [ 17 ]. Another strain isolated from Moroccan tannery waste assigned as A. faecalis BW1, its ethyl acetate extract showed antimycobacterial activity against Mycobacterium smegmatiss [ 19 ]. An isolated strain of A. faecalis from crude oil field in Malaysia showed inhibitory effect against Desulfovibrio sp., a sulfate reducing bacterium, using the isolate chloroform extract [ 24 ].…”

“…may influence the structure of produced secondary metabolites, such as antimicrobials [ 25 , 26 ]. In a study conducted by Kapley et al, the seed culture was carried out in M9 media [ 17 ] and then fermentation was completed in Luria-Bertani (LB) broth at 37 °C, at 150 rpm for 48 h producing antimicrobial [ 19 ]. Another study conducted on A. faecalis strain producing antimicrobial metabolites against Desulfovibrio sp., was cultivated in 1 L of marine broth under rotary agitation condition at 150 rpm at 30 °C for 96 h and metabolites were extracted by chloroform [ 24 ].…”

“…Few studies were published on the antimicrobial metabolites produced by A. faecalis [ 17 , 18 , 19 ]. Therefore, the present study aimed to discover the nature of the antimicrobial metabolites from A. faecalis isolate recovered from Egyptian soil by using conventional and chromatographic methods and correlating the obtained results with those of nanopore metagenomics biosynthetic gene clusters analysis which is a novel approach for deciphering the antimicrobial metabolites of soil microbiota.…”