Partial Purification and Characterization of Plantaricin Wzd3, a Bacteriocin Produced by Lactobacillus Plantarum Wzd3

Abstract

For partial purification of plantaricin WZD3, a bacteriocin produced by Lactobacillus plantarum WZD3 which has antifungal activity against Candida albicans, three procedures were performed; adsorption-desorption method, coldacetone extraction method and n-butanol extraction method. The results showed that n-butanol extraction method was the best since the activity of bacteriocin increased to 320 AU/ml. Characterization of plantaricin WZD3were also studied and the results showed that this bacteriocin have high thermostability at different temperatures, it remained active without losing its activity after being treated with (20-80) °C for (10, 30 and 60) minutes, but it retained only 50% of its activity after treatment at 100°C at the same periods of exposure and at 121°C for 15 minutes. Also, the activity of bacteriocin was stable at pH values (4-7) while at the pH values (8-11), the antifungal activity was decreased to the half, on the other hand, complete loss of activity was observed at pH (2, 3 and 12). Antifungal activity of plantaricin WZD3 was disappeared when it treated with proteolytic enzymes (pepsin and trypsin), whereas it retained whole activity when treated with lysozyme and α-amylase, indicating pure proteinaceous nature of it. The results of effect of storage period revealed that the activity of plantaricin WZD3 was gradually decreased during storage. In conclusion, plantaricin WZD3 could be a good candidate as antifungal agent and its properties are promising to use it as biopreservative instead of chemicals preservatives.