Partial Purification and Characterization of Six Transglutaminases from Ordinary Muscles of Various Fishes and Marine Invertebrates

Nozawa, Hisanori; Mamegoshi, Shinichi; Seki, Nobuo

doi:10.1016/s0305-0491(97)00062-x

Cited by 53 publications

(42 citation statements)

References 12 publications

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“…. A similar presence of transglutaminase was described in marine invertebrates (Nozawa, Mamegoshi, & Seki, 1997). Studies on cephalopods reported a decrease in gel strength due to the presence of proteolytic activity that degrades the myosin heavy chain (Nagashima, Ebina, Nagai, Tanaka, & Taguchi, 1992;Gomez-Guillen, Hurtado, & Montero, 2002).…”

Section: Discussionmentioning

confidence: 74%

Effect of protease inhibitors on thermal gelation of squid (Illex argentinus). mantle paste

2014

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The characteristics of the thermal gelation of squid mantle paste and the effect of protease inhibitors on them were investigated. Pastes in the absence and presence the protease inhibitors, ethylendiaminetetracetic acid (EDTA) and phenylmethylsulfonyl fluoride (PMSF), were formulated. Pastes were made by the respective one or two step thermal treatments: direct heating at 85 o C for 20 min and preincubation at 27 or 40 o C for 3 or 2 hours, followed by heating at 85 o C for 20 min. The gel strength, water holding capacity (WHC) and whiteness of gelled pastes were analyzed. The tricloroacetic acid (TCA) soluble peptides in homogenate of the muscle were determined. Gel strength decreased when heating was made in two steps. EDTA and PMSF were effective in avoiding that decrease when preincubation was made at 40 o C. Maximum gel strength was observed for the gels in presence of EDTA, giving values of 255 and 219 g x cm for the samples made by direct heating and pre-incubated at 40 o C respectively. TCA soluble peptides increased between 20 and 60 o C, with maximum values reached at 30 and 60 o C. No significant differences (p>0.05) were observed in gel whiteness, neither with the thermal treatment nor with the inhibitors. The WHC was higher (p<0.05) in the gelated paste formulated with EDTA. These results show a good gelation capacity of I. argentinus pastes and improvements with protease inhibitors.

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Section: Discussionmentioning

confidence: 74%

Effect of protease inhibitors on thermal gelation of squid (Illex argentinus). mantle paste

2014

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“…TGase 2 may contribute to the stability of the postsynaptic cholinergic system, since it was detected at neuromuscular junctions during normal neural development (17). Also, it cross-links muscle proteins in different systems (18,19). Furthermore, one study using fetal rat myotubes reported the presence of equivalent amounts of particulate and cytosolic TGase activity (16), but detailed characterization was not performed.…”

Section: Discussionmentioning

confidence: 99%

Sporadic Inclusion Body Myositis Correlates with Increased Expression and Cross-linking by Transglutaminases 1 and 2

Choi

Park

Kim

et al. 2000

Journal of Biological Chemistry

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Sporadic inclusion body myositis (SIBM) is characterized by vacuolar degeneration of muscle fibers and intrafiber clusters of paired helical filaments with abnormal amyloid deposition. Because of their potential involvement in other degenerative disorders, we have examined the expression of transglutaminases (TGases) in normal and SIBM tissues. We report that at least two different enzymes, the ubiquitous TGase 2 as well as the TGase 1 enzyme, are present in muscle tissues. However, in comparison with normal tissue, the expression of TGases 1 and 2 was increased 2.5-and 4-fold in SIBM, accompanied by about a 20-fold higher total TGase activity. By immunohistochemical staining, in normal muscle, TGase 2 expression was restricted to some endomysial connective tissue elements, whereas TGase 1 and ␤-amyloid proteins were not detectable. In SIBM muscle, both TGases 1 and 2 as well as amyloid proteins were brightly expressed and co-localized in the vacuolated muscle fibers, but none of these proteins colocalized with inflammatory cell markers. Next, we isolated high molecular weight insoluble proteins from SIBM muscle tissue and showed that they were cross-linked by about 6 residues/1000 residues of the isopeptide bond. Furthermore, by amino acid sequencing of solubilized tryptic peptides, they contain amyloid and skeletal muscle proteins. Together, these findings suggest that elevated expression of TGases 1 and 2 participate in the formation of insoluble amyloid deposits in SIBM tissue and in this way may contribute to progressive and debilitating muscle disease.

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“…In addition, it acts as a catalyst for acyl transfer reaction between the carboxamide groups of Gln residues and primary amines in proteins [1][2][3]. TGase is known to exist in fish muscle [4,5]. It has been reported that this kind of endogenous TGase in muscle mediates the specific cross-linking of myosin heavy chain in actomyosin [6].…”

Section: Introductionmentioning

confidence: 99%

Identification of the glutamine residue that may be involved in the transglutaminase-mediated intramolecular crosslinking of carp and walleye pollack myosin

Nozawa

Ezou

2009

Fish Sci

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In order to elucidate the molecular mechanism of transglutaminase-mediated myosin cross-linking, a fluorescent monodansylcadaverine (MDC) was incorporated into carp Cyprinus carpio myosin and the reactive Gln residues were analyzed by cyanogen bromide cleavage. The fluorescence was predominantly detected in 10.5 kDa BrCN-fragment, which is assumed to be located in subfragment 2 of myosin heavy chain. Furthermore, lysyl endopeptidase digestion of the 10.5 kDa fragment revealed that MDC was specifically incorporated into 520th Gln residue of the subfragment 2 domain. When meat paste prepared from walleye pollack Theragra chalcogramma frozen surimi was incubated with MDC, the fluorescence was mostly observed in 16 kDa BrCN-fragment and also slightly detected in other three bands. By the digestion of 16 kDa fragment with lysyl endopeptidase, it was elucidated that MDC was incorporated specifically into Gln-520 of myosin subfragment 2 as well as detected in carp. This domain around Gln-520 is likely to be a common critical region for dimer formation of myosin heavy chains for both fish species. In walleye pollack, other reactive Gln residues are presumed to be exist in the C-terminus of the light meromyosin. This slight difference may be significant in a capacity to form tetramers or even larger multimers.3

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Partial Purification and Characterization of Six Transglutaminases from Ordinary Muscles of Various Fishes and Marine Invertebrates

Cited by 53 publications

References 12 publications

Effect of protease inhibitors on thermal gelation of squid (Illex argentinus). mantle paste

Effect of protease inhibitors on thermal gelation of squid (Illex argentinus). mantle paste

Sporadic Inclusion Body Myositis Correlates with Increased Expression and Cross-linking by Transglutaminases 1 and 2

Identification of the glutamine residue that may be involved in the transglutaminase-mediated intramolecular crosslinking of carp and walleye pollack myosin

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