Partial redundancy in the synthesis of the d-arabinose incorporated in the cell wall arabinan of Corynebacterineae

Méniche, Xavier; Silva, Célia Regina Sousa da; Van-der-Rest, Bénoit; Bhamidi, Suresh; Huc, Emilie; Huang, Hairong; Paepe, Diane de; Tropis, Marielle; McNeil, Mike; Daffé, Mamadou; Houssin, Christine

doi:10.1099/mic.0.2008/016378-0

Cited by 34 publications

(35 citation statements)

References 45 publications

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“…Arabinose and galactose were found almost exclusively in F2 in a ratio of about 1:3.5 (Fig. 3C), which is in good agreement with the determined ratio in AG reported in previous studies of crude cell walls of C. glutamicum (38). Galactosamine was reported to be linked to the AG chain of slow-growing mycobacteria (19).…”

Section: Resultssupporting

confidence: 80%

Biochemical Disclosure of the Mycolate Outer Membrane of Corynebacterium glutamicum

et al. 2012

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Corynebacterineae is a specific suborder of Gram-positive bacteria that includes Mycobacterium tuberculosis and Corynebacterium glutamicum. The cell wall of these bacteria is composed of a heteropolymer of peptidoglycan (PG) linked to arabinogalactan (AG), which in turn is covalently associated with an atypical outer membrane, here called mycomembrane (M). The latter structure has been visualized by cryo-electron microscopy of vitreous sections, but its biochemical composition is still poorly defined, thereby hampering the elucidation of its physiological function. In this report, we show for the first time that the mycomembrane-linked heteropolymer of PG and AG (M-AG-PG) of C. glutamicum can be physically separated from the inner membrane on a flotation density gradient. Analysis of purified M-AG-PG showed that the lipids that composed the mycomembrane consisted almost exclusively of mycolic acid derivatives, with only a tiny amount, if any, of phospholipids and lipomannans, which were found with the characteristic lipoarabinomannans in the plasma membrane. Proteins associated with or inserted in the mycomembrane were extracted from M-AG-PG with lauryl-dimethylamine-oxide (LDAO), loaded on an SDS-PAGE gel, and analyzed by tandem mass spectrometry or by Western blotting. Sixty-eight different proteins were identified, 19 of which were also found in mycomembrane fragments released by the terminal-arabinosyl-transferase-defective ⌬AftB strain. Almost all of them are predicted to contain a signal sequence and to adopt the characteristic ␤-barrel structure of Gram-negative outer membrane proteins. These presumed mycomembrane proteins include the already-known pore-forming proteins (PorA and PorB), 5 mycoloyltransferases (cMytA, cMytB, cMytC, cMytD, and cMytF), several lipoproteins, and unknown proteins typified by a putative C-terminal hydrophobic anchor.

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Section: Resultssupporting

confidence: 80%

Biochemical Disclosure of the Mycolate Outer Membrane of Corynebacterium glutamicum

et al. 2012

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“…DPA is synthesized from phosphoribose diphosphate through a series of three successive reactions (Meniche et al 2008): (a) transfer of phosphoribose diphosphate to decaprenyl phosphate to form decaprenylphosphoryl-5-phosphoribose, (b) removal of the 5′ phosphate to give DPR, and (c) epimerization of DPR into DPA. This sugar is used as the only donor of Araf residues in the biosynthesis of both mycobacteria and corynebacteria cell wall (Mikusova et al 2005) which is composed of three main portions: a highly impermeable layer of mycolic acids, the complex polysaccharide arabinogalactan, and a peptidoglycan layer.…”

Section: The Decaprenyl-phosphoribose 2′-epimerasementioning

confidence: 99%

RETRACTED ARTICLE: Antituberculars which target decaprenylphosphoryl-β-D-ribofuranose 2′-oxidase DprE1: state of art

Buroni

Pasca

Ribeiro

et al. 2012

Appl Microbiol Biotechnol

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Multidrug resistance is a major barrier in the battle against tuberculosis and still a leading cause of death worldwide. In order to fight this pathogen, two routes are practicable: vaccination or drug treatment. Vaccination against Mycobacterium tuberculosis with the current vaccine Mycobacterium bovis Bacillus Calmette-Guerin is partially successful, being its efficacy variable. A few new tuberculosis vaccines are now in various phases of clinical trials. The emergence of multidrug-resistant strains of M. tuberculosis gave the impulse to discover new effective antitubercular drugs, a few of which are in clinical development. Here we focus on three different classes of very promising antitubercular drugs recently discovered (benzothiazinones, dinitrobenzamides, and benzoquinoxalines) that share the same cellular target: a subunit of the heteromeric decaprenylphosphoryl-β-D: -ribose 2'-epimerase, encoded by the dprE1 (or Rv3790) gene. This enzyme is involved in the biosynthesis of D: -arabinose which is crucial for the synthesis of the mycobacterial cell wall and essential for the pathogen's survival.

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“…(Leoff, et al, 2008) Corynebacterium glutamicum Arabinogalactan R-TOF (DHB) Study of enzymology involved in biosynthesis (Meniche, et al, 2008) Equisetum arvense (horsetail) Cell walls TOF, Glycans (perMe)…”

Section: Various Polysaccharidesmentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2007–2008

Harvey

2011

Mass Spectrometry Reviews

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This review is the fifth update of the original review, published in 1999, on the application of MALDI mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2008. The first section of the review covers fundamental studies, fragmentation of carbohydrate ions, use of derivatives and new software developments for analysis of carbohydrate spectra. Among newer areas of method development are glycan arrays, MALDI imaging and the use of ion mobility spectrometry. The second section of the review discusses applications of MALDI MS to the analysis of different types of carbohydrate. Specific compound classes that are covered include carbohydrate polymers from plants, N- and O-linked glycans from glycoproteins, biopharmaceuticals, glycated proteins, glycolipids, glycosides and various other natural products. There is a short section on the use of MALDI mass spectrometry for the study of enzymes involved in glycan processing and a section on the use of MALDI MS to monitor products of the chemical synthesis of carbohydrates with emphasis on carbohydrate-protein complexes and glycodendrimers. Corresponding analyses by electrospray ionization now appear to outnumber those performed by MALDI and the amount of literature makes a comprehensive review on this technique impractical. However, most of the work relating to sample preparation and glycan synthesis is equally relevant to electrospray and, consequently, those proposing analyses by electrospray should also find material in this review of interest.

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Partial redundancy in the synthesis of the d-arabinose incorporated in the cell wall arabinan of Corynebacterineae

Cited by 34 publications

References 45 publications

Biochemical Disclosure of the Mycolate Outer Membrane of Corynebacterium glutamicum

Biochemical Disclosure of the Mycolate Outer Membrane of Corynebacterium glutamicum

RETRACTED ARTICLE: Antituberculars which target decaprenylphosphoryl-β-D-ribofuranose 2′-oxidase DprE1: state of art

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2007–2008

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