Particle bombardment technology and its applications in plants

Özyiğit, İ̇brahim İ̇lker; Kurtoğlu, Kuaybe Yücebilgili

doi:10.1007/s11033-020-06001-5

Cited by 56 publications

(25 citation statements)

References 135 publications

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“…SiO 2 NPs have been developed to transport DNA to crops, e.g., tobacco and maize ( Zea mays L.) with no adverse effects [ 46 ]. The technology of particle bombardment (or gene-gun) has been used to transform plants since the early 1980s, using either tungsten or gold particles as DNA carriers [ 47 ]. The current system is adapted to allow NPs delivery, which most likely decreases the cell damage caused by microprojectile hits during the bombardment and thus improves the expression efficiency of the transgenes.…”

Section: Nanotechnology In Genetic Engineering and Crop Breedingmentioning

confidence: 99%

Phytonanotechnology applications in modern agriculture

et al. 2021

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With the rapidly changing global climate, the agricultural systems are confronted with more unpredictable and harsh environmental conditions than before which lead to compromised food production. Thus, to ensure safer and sustainable crop production, the use of advanced nanotechnological approaches in plants (phytonanotechnology) is of great significance. In this review, we summarize recent advances in phytonanotechnology in agricultural systems that can assist to meet ever-growing demands of food sustainability. The application of phytonanotechnology can change traditional agricultural systems, allowing the target-specific delivery of biomolecules (such as nucleotides and proteins) and cater the organized release of agrochemicals (such as pesticides and fertilizers). An amended comprehension of the communications between crops and nanoparticles (NPs) can improve the production of crops by enhancing tolerance towards environmental stresses and optimizing the utilization of nutrients. Besides, approaches like nanoliposomes, nanoemulsions, edible coatings, and other kinds of NPs offer numerous selections in the postharvest preservation of crops for minimizing food spoilage and thus establishing phtonanotechnology as a sustainable tool to architect modern agricultural practices. Graphical Abstract

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Section: Nanotechnology In Genetic Engineering and Crop Breedingmentioning

confidence: 99%

Phytonanotechnology applications in modern agriculture

et al. 2021

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“…The principle of the gene gun method is to use accelerators to transfer particles coated with exogenous genes into receptor cells, tissues, or organs so that the exogenous genes can be integrated into the receptor genome and expressed (Zhang et al, 2013). This method is mainly applied to crops and some fruit trees such as wheat, corn, bean, and citrus (Ozyigit and Yucebilgili Kurtoglu, 2020). Compared to A. tumefaciens-mediated transformation, the gene gun method is not limited by genotype (Ozyigit and Yucebilgili Kurtoglu, 2020).…”

Section: Gene Gun-mediated Transformationmentioning

confidence: 99%

“…This method is mainly applied to crops and some fruit trees such as wheat, corn, bean, and citrus (Ozyigit and Yucebilgili Kurtoglu, 2020). Compared to A. tumefaciens-mediated transformation, the gene gun method is not limited by genotype (Ozyigit and Yucebilgili Kurtoglu, 2020). The applications of gene gunmediated transformation in forest trees are listed in Table 3; gene gun technology has considerable potential in forest tree research.…”

Section: Gene Gun-mediated Transformationmentioning

confidence: 99%

Advances and Perspectives of Transgenic Technology and Biotechnological Application in Forest Trees

et al. 2021

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Transgenic technology is increasingly used in forest-tree breeding to overcome the disadvantages of traditional breeding methods, such as a long breeding cycle, complex cultivation environment, and complicated procedures. By introducing exogenous DNA, genes tightly related or contributed to ideal traits—including insect, disease, and herbicide resistance—were transferred into diverse forest trees, and genetically modified (GM) trees including poplars were cultivated. It is beneficial to develop new varieties of GM trees of high quality and promote the genetic improvement of forests. However, the low transformation efficiency has hampered the cultivation of GM trees and the identification of the molecular genetic mechanism in forest trees compared to annual herbaceous plants such as Oryza sativa. In this study, we reviewed advances in transgenic technology of forest trees, including the principles, advantages and disadvantages of diverse genetic transformation methods, and their application for trait improvement. The review provides insight into the establishment and improvement of genetic transformation systems for forest tree species. Challenges and perspectives pertaining to the genetic transformation of forest trees are also discussed.

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“…The ability of particle bombardment to mediate transient transgene expression is well-recognized ( Takemoto and Jones, 2014 ; Ozyigit and Yucebilgili Kurtoglu, 2020 ). Hence, Zhang et al developed a transiently expressed CRISPR/Cas9 DNA (TECCDNA)-based genome editing system to avoid transgene integration ( Zhang et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Transgene-free Genome Editing in Plants

Liu

Zhang

2021

Front. Genome Ed.

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Genome editing is widely used across plant species to generate and study the impact of functional mutations in crop improvement. However, transgene integration in plant genomes raises important legislative concerns regarding genetically modified organisms. Several strategies have been developed to remove or prevent the integration of gene editor constructs, which can be divided into three major categories: 1) elimination of transgenic sequences via genetic segregation; 2) transient editor expression from DNA vectors; and 3) DNA-independent editor delivery, including RNA or preassembled Cas9 protein-gRNA ribonucleoproteins (RNPs). Here, we summarize the main strategies employed to date and discuss the advantages and disadvantages of using these different tools. We hope that our work can provide important information concerning the value of alternative genome editing strategies to advance crop breeding.

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Particle bombardment technology and its applications in plants

Cited by 56 publications

References 135 publications

Phytonanotechnology applications in modern agriculture

Phytonanotechnology applications in modern agriculture

Advances and Perspectives of Transgenic Technology and Biotechnological Application in Forest Trees

Transgene-free Genome Editing in Plants

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