Particle Enhanced Light Scattering Immunoassay

Newman, David J.; Henneberry, H.P.; Price, Christopher P.

doi:10.1177/000456329202900104

Cited by 70 publications

(46 citation statements)

References 79 publications

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“…Alternatively mice received 0.2, 2, 20, or 200 mg GT, prior to the above procedures. Serum levels of GTTR and gentamicin were determined using standard diagnostic particleenhanced turbidimetric inhibition immunoassay methods (Newman et al 1992) on a Beckman Synchron Drug Calibrator 3 Plus, with a sensitivity of 0.5 mg/ml.…”

Section: Methodsmentioning

confidence: 99%

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Trafficking of Systemic Fluorescent Gentamicin into the Cochlea and Hair Cells

Wang

Steyger

2009

JARO

130

212

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Aminoglycosides enter inner ear hair cells across their apical membranes via endocytosis, or through the mechanoelectrical transduction channels in vitro, suggesting that these drugs enter cochlear hair cells from endolymph to exert their cytotoxic effect. We used zebrafish to determine if fluorescently tagged gentamicin (GTTR) also enters hair cells via apically located calcium-sensitive cation channels and the cytotoxicity of GTTR to hair cells. We then examined the serum kinetics of GTTR following systemic injection in mice and which murine cochlear sites preferentially loaded with systemically administered GTTR over time by confocal microscopy. GTTR is taken up by, and is toxic to, wild-type zebrafish neuromast hair cells. Neuromast hair cell uptake of GTTR is attenuated by high concentrations of extracellular calcium or unconjugated gentamicin and is blocked in mariner mutant zebrafish, suggestive of entry via the apical mechanotransduction channel. In murine cochleae, GTTR is preferentially taken up by the stria vascularis compared to the spiral ligament, peaking 3 h after intra-peritoneal injection, following GTTR kinetics in serum. Strial marginal cells display greater intensity of GTTR fluorescence compared to intermediate and basal cells. Immunofluorescent detection of gentamicin in the cochlea also revealed widespread cellular labeling throughout the cochlea, with preferential labeling of marginal cells. Only GTTR fluorescence displayed increasing cytoplasmic intensity with increasing concentration, unlike the cytoplasmic intensity of fluorescence from immunolabeled gentamicin. These data suggest that systemically administered aminoglycosides are trafficked from strial capillaries into marginal cells and clear into endolymph. If so, this will facilitate electrophoretically driven aminoglycoside entry into hair cells from endolymph. Trans-strial trafficking of aminoglycosides from strial capillaries to marginal cells will be dependent on as-yet-unidentified mechanisms that convey these drugs across the intra-strial electrical barrier and into marginal cells.

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Section: Methodsmentioning

confidence: 99%

“…The serum levels of GT and GTTR were detected by particle-enhanced turbidimetric inhibition immunoassay (Newman et al 1992). Within 10 min after i.p.…”

Section: Gttr Has Longer Serum Kinetics Than Gentamicinmentioning

confidence: 99%

Trafficking of Systemic Fluorescent Gentamicin into the Cochlea and Hair Cells

Wang

Steyger

2009

JARO

130

212

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“…Test-card latex particle agglutination (LPA) is usually read by eye as a qualitative indicator in medical diagnostic laboratories [9], although quantitative assays that rely on turbidimetric methods can determine analyte concentration [10]. Standard agglutination methods are perceived in some cases to be insensitive, non-quantitative and dif®cult to interpret [11±16].…”

Section: Introductionmentioning

confidence: 99%

Diagnostic particle agglutination using ultrasound: a new technology to rejuvenate old microbiological methods

Ellis¹,

Sobański

2000

Journal of Medical Microbiology

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“…Appropriate kits were supplied by Siemens Company (Germany). The liver enzymes (ALP, ALT, AST) found in the serum were analyzed using a DXC 800 Beckman Coulter autoanalyzer (Newman et al, 1992;Young and Bermes, 1999). Reactants for all the measurements were supplied by Beckman Coulter Company (USA).…”

Section: Analyses Of Blood Parametersmentioning

confidence: 99%

Effects of aluminum, copper, and titanium nanoparticles onsome blood parameters in Wistar rats

Canli¹,

Canli²

2017

Turk J Zool

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IntroductionFollowing recent technological developments, nanosized (<100 nm) metal complexes have entered into different areas of human lives and the discussion of their potential effects on humans and the environment has begun (Jeng and Swanson, 2006;Janrao et al., 2014). The areas in which nanoparticles (NPs) are used for anthropogenic purposes include medicine, the textile and electronic industries, filters, toothpaste, suntan cream, toys, moisturizers, packing products, household appliances, and the food industry. Although they are widely used, there are no satisfactory data on the environmental fate of these particles, despite vigorous attempts to find out their possible effects in different groups of animals. Those studies indicated that NPs may possess health hazard effects, domestically, medically, and environmentally, due to their high surfaceto-volume ratio, small size, crystallinity, electronic properties, surface structure, reactivity, functional groups, inorganic or organic coatings, shape, and aggregation behavior, which can make the particles very reactive or catalytic (Keller et al., 2010;Schrand et al., 2010;Janrao et al., 2014). NPs are able to pass through cell membranes and their interactions with biological systems are relatively unknown (Ahamed et al., 2010; Jeng and Swanson, 2014). Therefore, NPs can potentially cause adverse effects on organs, tissues, cellular and subcellular functions, and enzymes due to their unusual physicochemical properties (Keller et al., 2010;Schrand et al., 2010). Studies have shown that NPs have toxic effects on mammals depending on metal type, size, dose, and administration route of these particles (Elle et al., 2013;Wang et al., 2013; Jeng and Swanson, 2014). Although the literature data present so far are not consistent and do not show clear evidence of the toxicity of NPs, it seems they are not innocent products of mammalian metabolism. Biomarkers belonging to different metabolic systems in mammals were altered by NPs administrated via the oral route, inhalation, or subcutaneous injection (

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Particle Enhanced Light Scattering Immunoassay

Cited by 70 publications

References 79 publications

Trafficking of Systemic Fluorescent Gentamicin into the Cochlea and Hair Cells

Trafficking of Systemic Fluorescent Gentamicin into the Cochlea and Hair Cells

Diagnostic particle agglutination using ultrasound: a new technology to rejuvenate old microbiological methods

Effects of aluminum, copper, and titanium nanoparticles onsome blood parameters in Wistar rats

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