Particle Formation in Dextran Solutions

Ewald, Roger A.; Young, Allen A.; Crosby, William H.

doi:10.1093/milmed/129.10.952

Cited by 10 publications

(4 citation statements)

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“…22 Some polymer solutions, including dextran-containing, can undergo demixing, resulting in the formation of at least two phases: polymer-rich and polymer-depleted. [55][56][57] The phases can follow glass transitions, thus lowering the possibility of salt eutectic and protecting cells from 'solution effects'. Interestingly, more than one glass transition was shown for some dextran-based solution.…”

Section: Discussionmentioning

confidence: 99%

“…Polymer solutions are also good glass formers 22 . Some polymer solutions, including dextran‐containing, can undergo demixing, resulting in the formation of at least two phases: polymer‐rich and polymer‐depleted 55–57 . The phases can follow glass transitions, thus lowering the possibility of salt eutectic and protecting cells from ‘solution effects’.…”

Section: Discussionmentioning

confidence: 99%

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Open‐source hardware‐ and software‐based cryomicroscopy system for investigation of phase transitions in cryobiological research

Pakhomov,

Shevchenko,

Chernobai

et al. 2023

Journal of Microscopy

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The development of inexpensive equipment adapted for the study of a specific biological object is very important for cryobiology. In the presented work, we have proposed a simple system for microscopy utilising open‐source platform Arduino. Testing this system showed that it had sufficient sensitivity to determine the physical processes occurring in a cryopreserved sample such as intra‐ and extracellular water crystallisation and salt eutectic. Utilising this system, we investigated the mechanisms of cryoprotection and cryodamage of testis interstitial cells (ICs) in cryoprotective media, which included cryoprotective agents such as dimethyl sulphoxide (Me2SO), as well as foetal bovine serum or polymers (dextran, hydroxyethyl starch and polyethylene glycol). It was shown that a serum‐/xeno‐free medium that included 0.7 M Me2SO and 100 mg/mL dextran was able to reduce intracellular water crystallisation in cells, change the structure of extracellular ice, and reduce salt eutectic and recrystallisation. All these effects correlated with better IC survival after cryopreservation in the medium. This medium is potentially less toxic as it has lower concentrations of Me2SO compared to serum‐containing media developed for cryopreservation of testicular cells. This would pave a way for the creation of nontoxic serum‐free compositions that does not require removal before use of cryopreserved living cells for laboratory practice or in clinics.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Open‐source hardware‐ and software‐based cryomicroscopy system for investigation of phase transitions in cryobiological research

Pakhomov,

Shevchenko,

Chernobai

et al. 2023

Journal of Microscopy

View full text Add to dashboard Cite

show abstract

“…However, little attention has been paid to the stability of dextran solutions. A 10% dextran 40 solution, which is used as a blood plasma substitute, will develop precipitates in the injection vial during storage (1,2), indicating that dextran solutions are not stable. In fact, the formation of insoluble particles has been observed in bottled dextran solutions during sterilization and storage (1,2).…”

Section: Introductionmentioning

confidence: 99%