“…In recent years, due to advances in computers, optics, and digital image processing techniques, it has become possible to combine a conventional particle image velocimetry (PIV) system (Adrian 1991, Raffael et al 1998) with an inverted epifluorescent microscope (Santiago et al 1998, Koutsiaris et al 1999. As a result, this combination, known as a micro-PIV, has greatly increased the resolution of conventional PIV.…”