Partition of trypsin in two‐phase systems containing a diamidino‐α, ω‐diphenylcarbamyl poly (ethylene glycol) as competitive inhibitor of trypsin

Takerkart, G; Segard, E; Monsigny, Michel

doi:10.1016/0014-5793(74)80789-1

Cited by 51 publications

(5 citation statements)

References 8 publications

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“…Some examples are showed in table 1. In the early 1970s, Takerkart et al (1974) employed p-aminobenzamidine (PAB), a strong inhibitor of trypsin, as affinity ligand for the purification of trypsin. PEG9000-PAB exhibited a highly specific effect.…”

Section: The Other Ligands Affinity Partitioningmentioning

confidence: 99%

Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems

Souza

Pontes

et al. 2003

Braz. arch. biol. technol.

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From analytical to commercial scale, aqueous two-phase systems have their application in the purification, characterization and study of biomaterials. In order to improve the selectivity of the systems, the biospecific affinity ligands were introduced. In the affinity partitioning aqueous two-phase system, have many enzymes been purified. This review discusses the partitioning of some enzymes in the affinity aqueous two-phase systems in regard to the different ligands, including reactive dyes, metal ions and other ligands. Some integration of aqueous two-phasesystem with other techniques for more effective purification of enzymes are also presented.

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Section: The Other Ligands Affinity Partitioningmentioning

confidence: 99%

Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems

Souza

Pontes

et al. 2003

Braz. arch. biol. technol.

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“…This fact may be attributed to its unique properties such as chain flexibility, biocompatibility, non-immunogenicity, inert nature to most of biological molecules, and the basicity of the involved ether-oxygen into the main chain. , Again, the PEG substrate and its derivatives have extensive applications in the synthesis of peptides of polymeric drugs, , as carriers in polyamides − and antistatic agents . It also used to improve the activity of the enzyme-metal ion, − blood substitution, and blood circulation as well as a surface modifying agent of transition metal aluminides and for stabilization of water based on epoxy formulations . These advantages may be explained by the high hydrophilicity, no antigenicity, nontoxicity, and low cost of PEG along with the other mentioned properties.…”

Section: Introductionmentioning

confidence: 99%

“…8 It also used to improve the activity of the enzymemetal ion, 9−11 blood substitution, 12 and blood circulation 13 as well as a surface modifying agent of transition metal aluminides 14 and for stabilization of water based on epoxy formulations. 15 These advantages may be explained by the high hydrophilicity, no antigenicity, nontoxicity, and low cost of PEG along with the other mentioned properties.…”

Section: Introductionmentioning

confidence: 99%

Promising Biocompatible, Biodegradable, and Inert Polymers for Purification of Wastewater by Simultaneous Removal of Carcinogenic Cr(VI) and Present Toxic Heavy Metal Cations: Reduction of Chromium(VI) by Poly(ethylene glycol) in Aqueous Perchlorate Solutions

et al. 2020

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A spectrophotometric technique has been applied for studying the reduction of chromium(VI) by poly(ethylene glycol) (PEG) as water-soluble and nontoxic synthetic polymer at a constant ionic strength of 4.0 mol dm −3 in the absence and presence of the ruthenium(III) catalyst. In the absence of the catalyst, the reaction orders in [Cr(VI)] and [PEG] were found to be unity and fractional first orders, respectively. The oxidation process was found to be acid-catalyzed with fractional second order in [H + ]. The addition of Ru(III) was found to catalyze the oxidation rates with observation of zero-order reaction in [CrO 42− ] and fractional orders in both [PEG] and [Ru(III)], respectively. The PEG reduces the soluble toxic hexavalent Cr(VI) as a model pollutant to the insoluble nontoxic Cr(III) complex, which is known to be eco-friendly and more safer from the environmental points of view. The acid derivative of PEG was found to possess high affinity for the removal of poisonous heavy metal ions from contaminant matters by chelation. Formation of the 1:1 intermediate complex has been kinetically revealed. A consistent reaction mechanism of oxidation was postulated and discussed.

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“…Poly(oxyethylene)-dicarboxylic acids (POE-dicarboxylic acids) in particular are used in a wide range of applications such as water soluble polymer support for Merrifield type peptide syntheses, 1,2 polymeric drug carriers (prodrugs), [3][4][5][6][7] antistatic agents in polyamides, 8 enzyme-metal ion conjugates for improved stability and activity of enzymes, 9 -11 hemoglobin conjugates for oxygen carrying blood substitute, 12 lipid conjugates for enhanced longevity in blood circulation, 13 surface modifying agents for transition metal alluminides, 14 two phase aqueous separation of enzymes, 15 stabilizers for water based epoxy formulations, 16 and so on. Poly(oxyethylene)-dicarboxylic acids (POE-dicarboxylic acids) in particular are used in a wide range of applications such as water soluble polymer support for Merrifield type peptide syntheses, 1,2 polymeric drug carriers (prodrugs), [3][4][5][6][7] antistatic agents in polyamides, 8 enzyme-metal ion conjugates for improved stability and activity of enzymes, 9 -11 hemoglobin conjugates for oxygen carrying blood substitute, 12 lipid conjugates for enhanced longevity in blood circulation, 13 surface modifying agents for transition metal alluminides, 14 two phase aqueous separation of enzymes, 15 stabilizers for water based epoxy formulations, 16 and so on.…”

Section: Introductionmentioning

confidence: 99%

Single step room temperature oxidation of poly(ethylene glycol) to poly(oxyethylene)‐dicarboxylic acid

Lele¹,

Kulkarni²

1998

J. Appl. Polym. Sci.

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Poly(oxyethylene)-dicarboxylic acids were obtained in high yields by room temperature oxidation of poly(ethylene glycol)s of molecular weights in the 4000 -100,000 range using Jone's reagent (CrO 3 and H 2 SO 4 ) as the oxidizing agent. Oxidation by-products from the reaction mixture were eliminated by adsorbing chromium salts onto activated charcoal. The acid values of poly(oxyethylene)-dicarboxylic acids so synthesized were in agreement with the theoretical values. Poly(oxyethylene)-dicarboxylic acids were characterized by 1 H-NMR, IR spectroscopy. Spectral data were in agreement with the proposed structures of products. In summary, a convenient, one pot method for the synthesis of a wide range of poly(oxyethylene)-dicarboxylic acids was developed.

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Partition of trypsin in two‐phase systems containing a diamidino‐α, ω‐diphenylcarbamyl poly (ethylene glycol) as competitive inhibitor of trypsin

Cited by 51 publications

References 8 publications

Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems

Liquid-liquid extraction of enzymes by affinity aqueous two-phase systems

Promising Biocompatible, Biodegradable, and Inert Polymers for Purification of Wastewater by Simultaneous Removal of Carcinogenic Cr(VI) and Present Toxic Heavy Metal Cations: Reduction of Chromium(VI) by Poly(ethylene glycol) in Aqueous Perchlorate Solutions

Single step room temperature oxidation of poly(ethylene glycol) to poly(oxyethylene)‐dicarboxylic acid

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