Partitioning of anionic/nonionic polysaccharides in a segregative system of anionic polyelectrolyte mixtures in aqueous solution: Effects of polymer charge density and ionic strength of solution

Abstract

The partitioning of three polysaccharides, Dextran sulfate (DxS), λ‐Carrageenan (CAR), and Dextran (Dx), and its dependence on polymer charge density and ionic strength have been investigated in the segregative mixture of poly(sodium styrene sulfonate) (NaPSS) and sodium polyacrylate‐acrylamide copolymers, NaP(AA/AM), of variable charge density, in aqueous solutions. It is shown that the solubility of all the three may be zero or finite in one or both phases, but the preferred phase is determined by the charge density on the copolymer. When charge densities are equal on the phase forming polymers, the saccharides CAR and Dx favor the phase rich in PSS, but they have greater affinity for the polymer of lower charge density when the charge densities on phase polymers are unequal. The partitioning behavior of DxS is the opposite of the other two polysachharides with respect to which phase it has the greatest affinity for. The values of the partitioning coefficients show that the system may be potentially useful for the purification of polysaccharides and other macromolecules, if appropriate conditions are selected. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 100: 1728–1734, 2006

Polymer‐based alternative method to extract bromelain from pineapple peel waste

“…Pareto chart for the effects of variables PEG (1) and PAA (2) concentration, PEG (3) and PAA (4) molar mass, salt concentration (5), and temperature (6) on yield in the top phase (A) and purification factor (B) of bromelain extraction by ATPS (PEG/PAA). electrostatic interaction with anionic biomolecules.…”

“…Because of the high water content, ATPS have several advantages when compared with commonly used separation and purification techniques. They have a low interfacial tension, in addition to being nontoxic, nonflammable, biocompatible, and being easily scaled‐up without considerable changes in the efficiency of the process .…”