Passage of Host Serum Components, including Antibody, across the Digestive Tract of Dermacentor variabilis (Say)

Ackerman, Steven; Clare, F B; McGill, T. W.; Sonenshine, Daniel E.

doi:10.2307/3280459

Cited by 107 publications

(60 citation statements)

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“…The biggest amount of food is ingested in the second phase of feeding [22]. During feeding, serum components enter the midgut and haemolymph [23][24][25][26][27]. High titres of guinea-pig IgG antibodies were found in the saliva of both partially fed Rhipicephalus appendiculatus females and after 6 days of the feeding period [28,29].…”

Section: Discussionmentioning

confidence: 94%

Study of the non-parasitic stage in Ixodes ricinus after co-feeding with Dermacentor reticulatus in three infestations

Bartosik

Buczek

Borzęcki³

et al. 2017

Ann Agric Environ Med.

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It was proved that transmission of some pathogens may occur between infected and uninfected ticks co-feeding on a host in the absence of systemic infection. The effect was studied of co-feeding of two different tick species Ixodes ricinus and Dermacentor reticulatus on the course of the non-parasitic stage. Species chosen for the experiment may share their habitats and co-infest animals in nature. In the course of the study the process of egg maturation, oviposition and larval hatching were investigated. In order to estimate if co-feeding influence can counteract host immunity, three subsequent infestations of rabbits were analyzed. Mono-specific groups values of such a parameters as egg amount, number of eggs per 1 mg of female engorgement weight, female oviposition weight loss, hatching success were higher in inter-specific groups were compared. The results indicate that co-feeding with other tick species may partially reduce the influence of host resistance in subsequent infestation.

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Section: Discussionmentioning

confidence: 94%

Study of the non-parasitic stage in Ixodes ricinus after co-feeding with Dermacentor reticulatus in three infestations

Bartosik

Buczek

Borzęcki³

et al. 2017

Ann Agric Environ Med.

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“…It has been demonstrated previously that host antibodies can pass through the midgut barrier of ticks and circulate in the hemolymph [24][25][26]. Detection of positive bands for recombinant HlFERs on Western blot analysis using egg , a product of lipid peroxidation, and protein carbonyl (PC) resulting from protein oxidation were detected in the eggs and larvae of ticks from vaccinated rabbits using specific immunoblot detection kits.…”

Section: Discussionmentioning

confidence: 96%

Evaluation and comparison of the potential of two ferritins as anti-tick vaccines against Haemaphysalis longicornis

et al. 2014

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Background: Tick control is an essential aspect of controlling the spread of tick-borne diseases affecting humans and animals, but it presently faces several challenges. Development of an anti-tick vaccine is aimed at designing cost-effective and environmentally friendly protection against ticks and tick-borne diseases as an alternative to the use of chemical acaricides. A single vaccine from the tick midgut protein Bm86 is currently available for field applications, but its efficacy is limited to only some tick species. Identification of candidate vaccine antigens that can affect multiple tick species is highly desirable. The hard tick Haemaphysalis longicornis has two kinds of the iron-binding protein ferritin (HlFER), an intracellular HlFER1 and a secretory HlFER2, and RNA interference experiments showed that these are physiologically important in blood feeding and reproduction and in protection against oxidative stress. Here we investigated the potential of targeting HlFERs for tick control by immunizing the host with recombinant HlFERs (rHlFER1 and rHlFER2).Methods: Rabbits were immunized with rHlFERs three times subcutaneously at two-week intervals. Antisera were collected before the first immunization and a week after each immunization to confirm the antigen-specific serum antibody titer by serum ELISA. Two weeks after the final immunization, the rabbits were challenged with tick infestation. After dropping, tick feeding and reproduction parameters were evaluated to determine vaccine efficacy. To demonstrate the effects of antibodies, oxidative stress was detected in the eggs and larvae.Results: The antibody titer of rHlFER-immunized rabbits greatly increased after the second immunization. Antibodies exhibited cross-reactivity with rHlFERs and reacted with tick native HlFERs in Western blot analysis. Significantly lower bodyweight was observed in the ticks infested from the rHlFER2-immunized rabbit compared to those from the control rabbit. Reduced oviposition and hatching rate were observed in both rHlFER-immunized groups. rHlFER2 showed a higher vaccine efficacy. The antibodies against rHlFERs were detected in the eggs, and higher levels of oxidative stress biomarkers in the eggs and larvae, of ticks from rHlFER vaccinated rabbits. Conclusion:Collectively, these results showed that HlFER2 has a good potential as an anti-tick vaccine antigen that may affect multiple tick species.

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“…Guaragna et al (1992) imunossupressores como as prostaglandinas (RIBEIRO et al, 1985;INOKUMA et al, 1994;BOWMAN et al, 1995) e proteínas ligantes de imunoglobulinas (WANG et al, 1994), encontradas também na hemolinfa; anti-inflamatórios como os ligantes de histamina (PAESEN et al, 2000); inibidores da via clássica do sistema complemento como a calreticulina que se liga a C1q (SANDERS et al, 1999); inibidores da C3 convertase do complemento (VALENZUELA et al, 2000); inibidores de agregação plaquetária como as apirases WIKEL, 1996); e inibidoras de bradicinina (RIBEIRO e MATHER, 1998). (ACKERMAN et al, 1981;BROWN et al, 1982e BROWN, 1982e 1985. No caso do carrapato, o número de carrapatos alimentando-se de forma eficaz em um hospedeiro resistente é reduzido e as fêmeas têm o peso e a capacidade reprodutiva diminuídos (ACKERMAN et al, 1981;BROWN e ASKENASE, 1983;ASKENASE, 1984).…”

Section: Lista De Abreviaturas E Siglasunclassified

“…(ACKERMAN et al, 1981;BROWN et al, 1982e BROWN, 1982e 1985. No caso do carrapato, o número de carrapatos alimentando-se de forma eficaz em um hospedeiro resistente é reduzido e as fêmeas têm o peso e a capacidade reprodutiva diminuídos (ACKERMAN et al, 1981;BROWN e ASKENASE, 1983;ASKENASE, 1984).…”

Section: Lista De Abreviaturas E Siglasunclassified

“…A análise da hemolinfa de Dermacentor variabilis, que é um carrapato de cães, comprovou a presença de imunoglobulina G (IgG) com especificidade para as proteínas alvo em órgãos como glândula salivar e ovário (ACKERMAN et al, 1981 Sugere que a resistência ao carrapato pode ser devida a presença desses anticorpos contra antígenos salivares do carrapato, prevenindo contra transmissão de doença. (Craig et al, 1996;Wheeler et al, 1989) TABELA FUENTE et al, 1999).…”

Section: Lista De Abreviaturas E Siglasunclassified

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Identificação de antígenos do carrapato Rhipicephalus (Boophilus) microplus por soros de bovinos geneticamente resistentes e suscetíveis ao parasita

García¹

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AGRADECIMENTOSAgradeço primeiramente a Deus, pelo dom da vida, também pelo meu pai José Mário, meu irmão Alexandre (in Memoriam).Agradeço a toda minha família, meu irmão Rodrigo, meu sobrinho Pedro Henrique, minha tia Rosilene e meu tio Adauto, meus primos Gabriel e Raíssa, minha tia Sirleide, minha avó Dines e meu tio João; Em especial a minha mãe Sônia, minha avó Iva e Henrique Sales que são pessoas a quem eu devo muito do que eu sou hoje; muito obrigado a todos pelo incentivo e por tudo que fizeram por mim.A Dra. Isabel por ter me dado a oportunidade de participar do seu grupo de pesquisa; pela orientação e ajuda durante meu Mestrado; pela confiança depositada em mim e por compartilhar o seu conhecimento para com seus alunos. À Profa. Dra. Beatriz Rossetti Ferreira, pela co-orientação e auxílio indispensável durante meu aprendizado no laboratório e no desenvolvimento deste trabalho.Ao Prof. Dr. João Santana da Silva, por disponibilizar a estrutura de seu laboratório para realização deste trabalho, pelo profissionalismo, disciplina, pela coordenação, empenho e dedicação com o nosso programa de pós-graduação. À Profa. Dra. Ana Patrícia e seu grupo de trabalho, por disponibilizar a estrutura do seu laboratório e por proporcionar um ambiente agradável de trabalho.Aos técnicos Cristiane e Wander, pelo cuidado com o laboratório.A todos os alunos do laboratório de Imunoparasitologia da FMRP-USP que contribui direta ou indiretamente para este trabalho e sempre proporcionando um ambiente de trabalho descontraído. À Ana Cristine, pela amizade, ajuda e dedicação ao programa de pós-graduação da Imunologia, pela integração e disposição em ajudar os alunos.A todos os alunos do Programa de Pós-graduação de Imunologia Básica e Aplicada, pelo bom convívio, por partilhar tantos momentos de estudo e também de amizade.A todos meus amigos e em destaque: Sandra, pela amizade e orientação e ensinamento durante meu apoio técnico e também por auxiliar nos meus experimentos; Wanessa por todos os ensinamentos e ajuda que com certeza foram cruciais para o desenvolvimento deste trabalho, pelos conselhos, críticas, sugestões e pela amizade; Antônio que é uma pessoa que dispensa comentários e que não tenho palavras para agradecer; Elen pela amizade e incentivo; Carlo pelo conselho, amizade e ensinamento; Paulo, Diego e Djalma pela amizade; Maíra pelo apoio, muita paciência e incentivo, enfim a todos os amigos que eu encontrei e tomaram seus caminhos, que de maneira geral pela paciência, apoio e amizade que foram fundamentais para desenvolvimento deste trabalho; também a Raquel e Zilda pelo carinho, companheirismo, apoio e incentivo na fase final deste trabalho. The cattle tick, Rhipicephalus (Boophilus) microplus, causes enormous losses to animal health and production. Since it is a hematophagous parasite the tick expoliates its hosts and transmits diseases. Parasitism by the tick is mediated by its saliva, which inhibits the host's local homeostatic reactions to its bite. Hosts mount immune responses against this parasite, including antib...

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Passage of Host Serum Components, including Antibody, across the Digestive Tract of Dermacentor variabilis (Say)

Cited by 107 publications

References 0 publications

Study of the non-parasitic stage in Ixodes ricinus after co-feeding with Dermacentor reticulatus in three infestations

Study of the non-parasitic stage in Ixodes ricinus after co-feeding with Dermacentor reticulatus in three infestations

Evaluation and comparison of the potential of two ferritins as anti-tick vaccines against Haemaphysalis longicornis

Identificação de antígenos do carrapato Rhipicephalus (Boophilus) microplus por soros de bovinos geneticamente resistentes e suscetíveis ao parasita

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