Passive targeting and lung tolerability of enoxaparin microspheres for a sustained antithrombotic activity in rats

Ibrahim, Shaimaa S.; Osman, Rihab; Mortada, Nahed D.; Geneidy, Ahmed-Shawky; Awad, Gehanne A.S.

doi:10.1080/10717544.2016.1245368

Cited by 9 publications

(3 citation statements)

References 47 publications

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“…amine and thiol) could be incorporated into the surface layer of polydopamine (PDA) via Michal addition or Schiff base reactions (Lee et al., 2009 ; Park et al., 2014 ). For these reasons, we selected an amine-terminated PEG-FA copolymer as the targeting ligand incorporated into PDA coated CA-PLGA/NPs to ensure efficient passive accumulation (Ibrahim et al., 2017 ) and simple universal active targeting.…”

Section: Introductionmentioning

confidence: 99%

Co-delivery of docetaxel and bortezomib based on a targeting nanoplatform for enhancing cancer chemotherapy effects

et al. 2017

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Using facile polydopamine (PDA)-based surface modification and a pH-sensitive catechol-boronate binding mechanism, a novel drug delivery system was designed for the treatment of breast cancer. The system was able to achieve the following goals: active targeting, pH responsiveness, in vivo blood circulation for a prolonged period of time, and dual drug loading. After coating with PDA, the docetaxel (DTX)-loaded star-shaped copolymer cholic acid-poly(lactide-co-glycolide) nanoparticles (CA-PLGA@PDA/NPs) were functionalized with amino-poly(ethylene glycol)-folic acid (NH-PEG-FA) and bortezomib (BTZ) to form the targeting composition, DTX-loaded CA-PLGA@PDA-PEG-FA + BTZ/NPs. The novel NPs exhibited similar drug release characteristics compared to unfunctionalized CA-PLGA/NPs. Meanwhile, the incorporated NH-PEG-FA contributed to active targeting which was illustrated by cellular uptake experiments and biodistribution studies. Moreover, the pH responsive binding between BTZ and PDA was demonstrated to be effective to release BTZ at the tumor acidic environment for synergistic action with DTX. Both in vitro cytotoxicity and in vivo antitumor studies demonstrated that the novel nanoplatform exhibited the most suitable therapeutic effects. Taken together, the versatile PDA modified DTX-loaded CA-PLGA@PDA-PEG-FA + BTZ/NPs offered a promising chemotherapeutic strategy for enhancing breast cancer treatment.

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Section: Introductionmentioning

confidence: 99%

Co-delivery of docetaxel and bortezomib based on a targeting nanoplatform for enhancing cancer chemotherapy effects

et al. 2017

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“…In the sections of this group, the alveolar wall was damaged, and alveolar epithelial cells were disorganized; leucocytes, cell shed debris, and apoptotic cells were present in many alveoli. In the study of Ibrahim et al 27 , lungs showed normal histological structures of the bronchioles, air alveoli, and blood vessels in both groups with no histopathological alterations after injection of control and Enoxaparin. They have not observed emboli or tissue infarction, macro-or microscopically in any of the samples.…”

Section: Discussionmentioning

confidence: 85%

Investigation of the protective effect of enoxaparin and ticagrelor pretreatment against ischemia-reperfusion injury in rat lung tissue

Findik¹,

Yilmaz

Yazır

et al. 2019

Rev. Assoc. Med. Bras.

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SUMMARY OBJECTIVES This study was conducted to reveal the possible protective effects of ticagrelor and enoxaparin pretreatment against ischemia-reperfusion (IR)-induced injury on the lung tissue of a rat model. METHODS Wistar albino rats were randomly divided into 4 groups as follows: group-1 (control-sham), group-2 (control-saline+IR), group-3 (ticagrelor+IR), group-4 (enoxaparin+IR). Before the ischemic period, saline, ticagrelor, and enoxaparin were administered to the 2nd-4th groups, respectively. In these groups, IR injury was induced by clamping the aorta infrarenally for 2 h, followed by 4 h of reperfusion except group-1. After the rats were euthanized, the lungs were processed for histological examinations. Paraffin sections were stained with Haematoxylin&Eosin (H&E) for light microscopic observation. Apoptosis was evaluated by caspase-3 immunoreactivity. Data were statistically analyzed using the SPSS software. RESULTS In the lung sections stained with H&E, a normal histological structure was observed in group-1, whereas disorganized epithelial cells, hemorrhage, and inflammatory cell infiltration were seen in the alveolar wall in group-2. The histologic structure of the treatment groups was better than that of group-2. Caspase-3(+) apoptotic cells were noticeable in sections of group-2 and were lower in the treatment groups. In group-4, caspase-3 immunostaining was lower than in group-3. In group-2, apoptotic cells were significantly higher than in the other groups (p<0.001). CONCLUSION Based on the histological results, we suggested that both therapies ameliorated the detrimental effects of IR. Caspase-3 immunohistochemistry results also revealed that pre-treatment with enoxaparin gave better results in an IR-induced rat injury model. In further studies, other parameters such as ROS and inflammatory gene expressions should be evaluated for accurate results.

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“…Later, the formula below permitted the calculation of these nanoencapsulated substances. 14,15 EE(%) = − × Total initial drug Drug in supernatant Total initial drug 10 00…”

Section: Determination Of Entrapment Efficiency (Ee)mentioning

confidence: 99%

Production and Characterization of Melon Sulfated Pectin (Cucumis melo var. acidulus) Nanoparticles with Anticoagulant Potential

Oliveira¹,

Almeida²,

Uchoa³

et al. 2020

JYP

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Samples of sulfated and non-sulfated polysaccharides were provided by the Department of Chemistry, at the Federal University of Ceara. Unfractionated heparin (Hemofol 5000UI / ml-Lote18030036) was purchased commercially. The Kits and the commercial kits used in the coagulation tests (APTT, PT and TT) were purchased commercially (Bios Diagnóstica ®). Poly (lactic-co-glycolic acid) (PLGA) with a 50:50 lactide co-glycolide ratio (MW 40-75 kDa) and poly (vinyl alcohol) (PVA) (MW 13-23 kDa, 87-89% hydrolyzed) were purchased from Sigma-Aldrich.

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Passive targeting and lung tolerability of enoxaparin microspheres for a sustained antithrombotic activity in rats

Cited by 9 publications

References 47 publications

Co-delivery of docetaxel and bortezomib based on a targeting nanoplatform for enhancing cancer chemotherapy effects

Co-delivery of docetaxel and bortezomib based on a targeting nanoplatform for enhancing cancer chemotherapy effects

Investigation of the protective effect of enoxaparin and ticagrelor pretreatment against ischemia-reperfusion injury in rat lung tissue

Production and Characterization of Melon Sulfated Pectin (Cucumis melo var. acidulus) Nanoparticles with Anticoagulant Potential

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