Paternity testing and forensic DNA typing by multiplex STR analysis using ABI PRISM 310 Genetic Analyzer

El-Alfy, Sherif H.; El-Hafez, Ahmed F. Abd

doi:10.1016/j.jgeb.2012.05.001

Cited by 29 publications

(18 citation statements)

References 29 publications

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“…Seven years later, it contained 1046 individuals' DNA profiles from 869 crime scenes, 20 missing persons plus 1815 unidentified human remains [9]. The current study showed high level of awareness about the DNA database benefits.…”

Section: Discussionmentioning

confidence: 73%

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Knowledge and Attitude of Medical Students of Cairo University, Cairo, Egypt About National DNA Database

Ibrahim

Ali

2017

Inter J Med Toxic Foren Med

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Background: DNA database is used to identify individuals based on their specific DNA profiles. In Egypt, a definitive law describing and legalizing DNA related issues is absent; however, the Egyptian courts frequently deal with these issues. The legal authorities strongly suggest the impending need for detailed decrees in this regard that guard the safety of the whole Egyptian community. This paper aimed to assess the knowledge and attitude of Medical students about Egyptian National DNA Database (END).Methods: Data of this study were collected from 272 participants in the Faculty of Medicine, Cairo University using interviewer administered questionnaire. Results:The participants were 136 males and 136 females with mean (SD) age of 27(9) years. Majority of the participants (89.7%) were aware about national database out of their medical textbooks. Personal identification was the most chosen END benefit and database usefulness was the main motivation for them. About 29% suggested to start END with the detected crime scene stains and forensic doctors should freely access END. Conclusion:These collective points of views might be useful in preparing some international and common ethical standards for the development of DNA databases framework.

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Section: Discussionmentioning

confidence: 73%

“…It is not for me it is for criminal only 4 (9) No: Number tional DNA databases share similar socio-ethical concerns and comparable aims, such as decreasing crime, aiding in identification of offenders, and crime detection.…”

Section: (113)mentioning

confidence: 99%

Knowledge and Attitude of Medical Students of Cairo University, Cairo, Egypt About National DNA Database

Ibrahim

Ali

2017

Inter J Med Toxic Foren Med

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“…as the biological parent of a child or the same liation or at least 99% to con rm someone as the biological parent. Legally, a 99% or greater probability of a biological relationship is considered proof of paternity [17,18]. The inclusion of paternity comes from the fact that one of the child's alleles is identical to one of the alleles of the alleged father for all the markers studied.…”

Section: Discussionmentioning

confidence: 99%

Contribution of ABO-Rhesus/Electrophoresis of hemoglobin methods and Short Tandem Repeats analysis in the determination of paternity in Burkina Faso, West Africa

Millogo¹,

Soubeïga²,

BAZIE³

et al. 2020

Preprint

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Background: the establishment of filiation by the current ABO, HLA, MNS, Kells and serum tests, pose a real reliability problem. It is then necessary to combine these methods with or to use high-performance methods such as microsatellite genetic analysis or short tandem repeats. This study aimed to compare the short tandem repeat technique with ABO/Rhesus system in combination with electrophoresis of hemoglobin. Methods: Fourteen (14) contested paternity trios were investigated. Blood samples were collected to determine blood groups using the Beth-Vincent method and the type of hemoglobin by electrophoresis. Blood spots on FTA paper were used for the analysis of 16 STR loci (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818, FGA, Amel) by capillary electrophoresis on the ABI 31310 Genetic Analyzer. The generated sequences were analyzed with GeneMapper® software version 3.2.1. The data were analyzed to determine the paternity index and the probability of paternity. Results: Of the fourteen (14) trios studied, ten (10) cases were probable inclusion, three (03) cases were exclusion and one (01) case was an undetermined paternity outcome with the ABO-Rhesus/ electrophoresis of hemoglobin system. While the analysis of genetic polymorphisms in DNA gave five (05) inclusions versus nine (09) exclusions of paternity. Of the 10 probable inclusion cases given by the ABO-Rhesus/Electrophoresis of hemoglobin system, only 05 cases (50%) were confirmed for inclusion by Short tandem repeat analysis. Conclusion: The analysis of short tandem repeat with sixteen genetic markers is more reliable in determining paternity than ABO-Rhesus/hemoglobin electrophoresis techniques.

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“…33 Different primers are designed for the 14 recommended STR markers each with different fluorescent labels. 34 The amplified STR fragments are then separated by capillary electrophoresis, they are placed into wells within polyacrylamide gel, that once a current is run through, will separate the fragments based on their size. With smaller fragments travelling further than that of larger ones, as seen in Figure 14.…”

Section: Dna Amplification and Profilingmentioning

confidence: 99%

DNA and fingerprint recovery from an arson scene

O'Hagan¹,

Calder²

2020

FRCIJ

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The different methods of both DNA and fingerprint recovery from arson scenes are analysed within this literature. Arson is often used as a mode of concealment due to the wide misconception that fire destroys fundamental evidence. Advances in recent research and technologies have meant that techniques have been developed to further aid and enhance the recovery of evidence that has been exposed to the extreme conditions of a fire. In the forefront of all fire investigations where evidence recovery is paramount, the stages of the fire should be taken into consideration and pre-eminent soot removal techniques carried out in order to expose and visualise any evidence present. Although evaluation of current literature demonstrates that exceptional results from both fingerprints and DNA exposed to fire can be obtained, through different enhancement techniques, further research and developments are required in order to ensure these essential developments are standardised and mandatory to all arson scenes.

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Paternity testing and forensic DNA typing by multiplex STR analysis using ABI PRISM 310 Genetic Analyzer

Cited by 29 publications

References 29 publications

Knowledge and Attitude of Medical Students of Cairo University, Cairo, Egypt About National DNA Database

Knowledge and Attitude of Medical Students of Cairo University, Cairo, Egypt About National DNA Database

Contribution of ABO-Rhesus/Electrophoresis of hemoglobin methods and Short Tandem Repeats analysis in the determination of paternity in Burkina Faso, West Africa

DNA and fingerprint recovery from an arson scene

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