Pathogen detection: A perspective of traditional methods and biosensors

Lazcka, Olivier; Campo, F. Javier del; Muñoz, Francesc Xavier

doi:10.1016/j.bios.2006.06.036

Cited by 1,344 publications

(828 citation statements)

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“…In order to ensure food and water safety and quality, processing and detection methods are desirable that can be used in the field to yield rapid time to results (Min and Baeumner 2002, Baeumner et al 2003, Kost 2006, Lazcka et al 2007, Lounsbury et al 2013. Existing laboratory methods to detect many common relevant pathogens (such as immunoassays or qPCR) require specialized scientific equipment, a stable laboratory environment, a continuous refrigeration chain for reagents or antibodies, and/or specially trained staff to perform numerous manual steps, especially during the sample preparation procedure (Leonard et al 2003, Lazcka et al 2007, Schuurman et al 2007, Nugen and Baeumner 2008, Velusamy et al 2010, Zuo et al 2013.…”

Section: Introductionmentioning

confidence: 99%

“…Existing laboratory methods to detect many common relevant pathogens (such as immunoassays or qPCR) require specialized scientific equipment, a stable laboratory environment, a continuous refrigeration chain for reagents or antibodies, and/or specially trained staff to perform numerous manual steps, especially during the sample preparation procedure (Leonard et al 2003, Lazcka et al 2007, Schuurman et al 2007, Nugen and Baeumner 2008, Velusamy et al 2010, Zuo et al 2013. Automated sample preparation robots are commercially available, but they are expensive and often still require a specialized laboratory to perform manual pipetting steps for the downstream diagnostic reaction (Merel 2005, Lee et al 2010, Phillips et al 2012.…”

Section: Introductionmentioning

confidence: 99%

“…Automated sample preparation robots are commercially available, but they are expensive and often still require a specialized laboratory to perform manual pipetting steps for the downstream diagnostic reaction (Merel 2005, Lee et al 2010, Phillips et al 2012. They are therefore not practical for many users in outbreak locations, at the point -of-care or for lowthroughput testing laboratories (Baeumner 2003, Lazcka et al 2007.…”

Section: Introductionmentioning

confidence: 99%

“…Any automated diagnostic test for small-scale use should rely on a simpler, more robust technology (Baeumner et al 2003, Leonard et al 2003, Kost 2006, Lazcka et al 2007, Yager et al 2008, Hoehl et al 2012. T here has thus been an effort to develop low-cost, automated diagnostic biosensors (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…nanotechnology-based sensors (Zhang et al 2005) or microfluidic systems, such as droplet -based , Chiou et al 2013, centrifugal (Cho et al 2007), paper (Martinez et al 2009, Fu et al 2010, Zuo et al 2013, capillary (T akayama et al 1999, Yager et al 2008, Gervais and Delamarche 2009, pneumatic (Legendre et al 2006, Mohan et al 2013 devices and lateral flow assays (Yager et al 2008, Chen et al 2010, You et al 2011). For the past decade, this has led to numerous publications about promising, early-stage technologies (Baeumner et al 2003, Leonard et al 2003, Kost 2006, Lazcka et al 2007, Lucchi et al 2010, Asiello and Baeumner 2011, Duarte et al 2011, Karlsson et al 2011, Schudel et al 2011, Matlock-Colangelo and Baeumner 2012, Lounsbury et al 2013, Mohan et al 2013. Despite some commercially available systems (e.g.…”

Section: Introductionmentioning

confidence: 99%

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Centrifugal LabTube platform for fully automated DNA purification and LAMP amplification based on an integrated, low-cost heating system

Hoehl

Weißert

Dannenberg

et al. 2014

Biomed Microdevices

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Abstract:This paper introduces a disposable battery-driven heating system for loop-mediated isothermal DNA amplification (LAMP) inside a centrifugally-driven DNA p urification platform (LabT ube). We demonstrate LabT ube-based fully automated DNA purification of as low as 100 cell-equivalents of verotoxin-producing Escherichia coli (VT EC) in water, milk and apple juice in a laboratory centrifuge, followed by integrated and automated LAMP amplification with a reduction of hands-on time from 45 to 1min. The heating system consists of two parallel SMD thick film resistors and a NT C as heating and temperature sensing elements. They are driven by a 3V battery and controlled by a microcontroller. The LAMP reagents are stored in the elution chamber and the amplification starts immediately after the eluate is purged into the chamber. The LabT ube, including a microcontroller-based heating system, demonstrates contamination-free and automated sample-to-answer nucleic acid testing within a laboratory centrifuge. The heating system can be easily parallelized within one LabT ube and it is deployable for a variety of heating and electrical applications.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Centrifugal LabTube platform for fully automated DNA purification and LAMP amplification based on an integrated, low-cost heating system

Hoehl

Weißert

Dannenberg

et al. 2014

Biomed Microdevices

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Biosensors, Toxicity Monitoring

Ornatska

Ganesana

Andreescu

2009

Encyclopedia of Industrial Biotechnology

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The need for simple, rapid, cost‐effective, and field‐portable screening methods has boosted development in the field of biosensors. This article reviews recent advances in the field of toxicity biosensors for monitoring chemical and biological toxicants, with a focus on how such technologies can be adapted to toxicity monitoring. Current efforts in this direction are concentrated toward disposable devices for screening and semiquantitative analysis. These can be designed to detect a single specific analyte or can provide general or group toxicity screening, depending on the design parameters and the specificity of the biomolecule. Relevant, specific applications and the analytical performance of biosensors for toxicity monitoring of several classes of chemical toxins are discussed.

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Microbiological Detectors for Food Safety Applications

Alocilja

Pal

2008

Wiley Handbook of Science and Technology for Homeland Security

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The use of microorganisms as biological weapons has long been reported in history. The most recent was the deliberate release of B. anthracis spores through the postal system in the United States in October 2001, resulting in 22 cases of anthrax and five deaths. Furthermore, there are up to 33 million cases of human illness each year from microbial pathogens in the food supply in the United States alone, with an associated cost of $2‐4 billion in 2006. The complexity of the US food supply chain from cradle to grave provides numerous entry points and routes in which (inadvertent and intentional) contaminants and pathogens can be introduced into the nation's food system. Many of these pathogenic microorganisms have a very low infectious dose to cause fatality. The most vulnerable populations are the very young, the elderly, and immunocompromised individuals. Although highly sensitive, current methods of detection are laborious, require highly skilled personnel, and typically require 2–7 days to obtain conclusive results. Their results are not available in the time‐scale desired in food defense, food quality assurance, or clinical laboratory procedures, which have safety, cost, and quality implications. Rapid on‐site point‐of‐care detection methods for pathogens have hence become a necessity. Biosensors can play a major role in addressing the combined needs for near real‐time diagnosis, specificity, sensitivity, portability, electronic data transmission, field‐based, ease of use, and low cost requirements. This chapter presents biosensors for rapid microbial detection in complex food matrices of concern to homeland security and food safety.

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Pathogen detection: A perspective of traditional methods and biosensors

Cited by 1,344 publications

References 108 publications

Centrifugal LabTube platform for fully automated DNA purification and LAMP amplification based on an integrated, low-cost heating system

Centrifugal LabTube platform for fully automated DNA purification and LAMP amplification based on an integrated, low-cost heating system

Biosensors, Toxicity Monitoring

Microbiological Detectors for Food Safety Applications

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