Pathogen-Free Mouse Rederivation by IVF, Natural Mating and Hysterectomy

Sztein, Jorge; Kastenmayer, Robin J.; Perdue, K. A.

doi:10.1007/978-3-642-20792-1_25

Cited by 6 publications

(5 citation statements)

References 38 publications

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“…Rederivation by embryo transfer or in vitro fertilization can be used to eliminate adventitious pathogens in mice, including ectoparasites, but it is costly and time-consuming. 25,93,97 An effective method to eradicate D. musculi infestation would be beneficial for the maintenance of biosecurity in SPF colonies where mice are imported without rederivation.…”

mentioning

confidence: 99%

Ivermectin-compounded Feed Compared with Topical Moxidectin–Imidacloprid for Eradication of Demodex musculi in Laboratory Mice

Nashat

Arbona

Lepherd

et al. 2018

j am assoc lab anim sci

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Demodex musculi is a prostigmatid follicular mite that has rarely been reported in laboratory mice. Although prevalence of this species has not been assessed formally, we have found that many imported mouse strains from noncommercial sources harbor Demodex mites. To assess whether an acaricide can be used to eradicate this mite, infested immunocompromised mice were provided ivermectin-compounded (12 ppm) feed without restriction for 8 wk (n = 10), were treated topically with moxidectin and imidacloprid (MI; 3 and 13 mg/kg, respectively) weekly for 8 wk (n = 10), or remained untreated (n = 10). Mice were confirmed to be mite-infested before treatment and were tested after treatment by using fur plucks (FP), deep skin scrapes (DSS), and PCR analysis of fur swabs. In addition, the presence of mites was confirmed through skin biopsies at 2 study endpoints (1 wk [n = 5] and 12 wk [ n = 5] after treatment). Samples collected before treatment and from untreated mice were positive for D. musculi at all time points and by all test modalities. After treatment, all ivermectin-treated animals remained infested, whereas mice treated with MI were repeatedly negative by all test modalities. An additional shortened treatment trial revealed that 4 wk of MI (n = 7) was insufficient to eradicate mites. Neither treatment produced any evidence of adverse effects according to hematology, serum chemistry parameters, behavior, body weight, and histopathology. Of the 70 PCR assays performed in treated mice, 14 were positive when FP+DSS was negative. In 6 cases where PCR results were negative, 5 were positive by FP+DSS and a single sample was positive on skin biopsy. Although PCR analysis has a high detection rate for D. musculi, FP+DSS can further enhance the detection rate. In conclusion, topical MI administered for 8 consecutive weeks can safely eradicate D. musculi from an immunocompromised mouse strain.

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mentioning

confidence: 99%

Ivermectin-compounded Feed Compared with Topical Moxidectin–Imidacloprid for Eradication of Demodex musculi in Laboratory Mice

Nashat

Arbona

Lepherd

et al. 2018

j am assoc lab anim sci

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“…If the purpose of the IVF is to re-derive an animal model, these parthenogenic embryos and 2-cell embryos with a small blastomere should be avoided. Additionally, embryos should be washed 5 to 10 times into different wash drops using a new transfer pipette each time (Sztein Kastenmayer, & Perdue, 2011).…”

Section: Jax Protocol For Ivf Of Mouse Spermmentioning

confidence: 99%

Cryopreservation Protocols for Genetically Engineered Mice

et al. 2021

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Protocols for cryopreservation of mouse embryos and sperm are important for preserving genetically engineered mice (GEMs) used in research to study human development and diseases. Embryo cryopreservation is mainly carried out using either of two protocols: controlled gradual cooling or vitrification. Sperm cryopreservation protocols include two methodologies that are commonly referred to as JAX and CARD. Quality-control measures are necessary to ensure that GEMs are properly cryopreserved so that they can be retrieved for future use. An archiving system is also important in keeping proper records of frozen sperm and embryos. Frozen embryos and sperm are now preferred over live mice for shipping to distant locations. This article describes detailed protocols used in cryopreservation of mouse embryos and sperm, as well as their retrieval to live mice.

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“…The capacitated BDF1 spermatozoa were used for IVF (Byers et al , 2006; Sztein et al , 2011). In brief, the spermatozoa were adjusted to about 1–2 × 10 7 spermatozoa/ml, then 10 µl of spermatozoa were added to the HTF medium (Millipore Inc., Billerica, MA), a final concentration of about 2 × 10 5 spermatozoa/ml.…”

Section: Methodsmentioning

confidence: 99%

Motility, progressive motility score and DNA integrity of spermatozoa from cold-preserved mouse cauda epididymis

Jiang¹,

Zhu²,

et al. 2017

Zygote

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This study attempted to investigate and validate whether epididymis cold storage could be a suitable alternative for short-term preservation of spermatozoa. Mouse cauda epididymides and spermatozoa were preserved at 4-8°C from 1 day to 6 weeks. From days 1 to 10, motility and fertility were daily examined when motility loss occurred. From week 1, spermatozoa were used for intracytoplasmic sperm injection (ICSI) at weekly intervals to test their fertility, and spermatozoa DNA integrity was determined by comet assay. We found that motility and progressive motility scores gradually decreased with storage time. In nearly all spermatozoa, DNA integrity was maintained from days 1 to 10, but the percentage of spermatozoa with damaged DNA significantly increased from week 2 to week 6. Spermatozoa retained fertility until day 6, although fertility gradually decreased after day 3. From week 1 to week 5, fertilization rates by ICSI were more than 82.69% but decreased gradually after week 3. We found that spermatozoa preserved in the epididymis at 4-8°C had progressively lower motility, fertility and proportion of undamaged DNA, but could still fertilize oocytes. However, all the parameters of cold-preserved spermatozoa were completely inferior to that from cold-preserved cauda epididymides. The results imply that cold storage of cauda epididymides could be conducive to short-term preservation of spermatozoa, and the cold-stored spermatozoa can resist DNA denaturation, which is necessary for maintaining reproductive ability.

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Pathogen-Free Mouse Rederivation by IVF, Natural Mating and Hysterectomy

Cited by 6 publications

References 38 publications

Ivermectin-compounded Feed Compared with Topical Moxidectin–Imidacloprid for Eradication of Demodex musculi in Laboratory Mice

Ivermectin-compounded Feed Compared with Topical Moxidectin–Imidacloprid for Eradication of Demodex musculi in Laboratory Mice

Cryopreservation Protocols for Genetically Engineered Mice

Motility, progressive motility score and DNA integrity of spermatozoa from cold-preserved mouse cauda epididymis

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