Pathogen reduction in an algal-based wastewater treatment system employing Galdieria sulphuraria

Delanka-Pedige, H.M.K.; Munasinghe-Arachchige, S.P.; Cornelius, Jackson; Henkanatte-Gedera, S.M.; Tchinda, D.; Zhang, Yanyan; Nirmalakhandan, Nagamany

doi:10.1016/j.algal.2019.101423

Cited by 77 publications

(18 citation statements)

References 27 publications

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“…Our system of wastewater treatment also decreased microbial functions related to pathogenesis, and the analyses con rmed that the microalgae-containing SP3 reduced bacterial richness and diversity. Consistent with our ndings, [41] reported high performance of an algae system in reducing pathogenic bacteria.…”

Section: Discussionsupporting

confidence: 91%

On-Site Blackwater Treatment Fosters Microbial Groups and Functions to Efficiently and Robustly Recover Carbon and Nutrients

Kuramae

Dimitrov

Silva

et al. 2020

Preprint

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Background: Wastewater is considered as a renewable resource water and energy. An advantage of decentralized sanitation systems is the separation of the blackwater (BW) stream, which is highly contaminated with human pathogens, from the remaining household water. However, the composition and functions of the microbial community in BW are not known. In this study, we used shotgun metagenomics to assess the dynamics of microbial community structure and function throughout a new BW anaerobic digestion system installed at The Netherlands Institute of Ecology. Samples from the influent (BW), primary effluent (anaerobic digested BW), sludge and final effluent of the pilot upflow anaerobic sludge blanket (UASB) reactor and microalgae pilot tubular photobioreactor (PBR) were analyzed.Results: Our results showed a decrease in microbial richness and diversity followed by a decrease in functional complexity and co-occurrence along the different modules of the bioreactor. The microbial diversity and function decrease were reflected both changes in substrate composition and wash conditions. The most prevalent core functions in influent (BW) were related to metabolism of carbohydrates, response to chemicals and drugs, and nitrogen. The core functions in anaerobic digested BW and upflow anaerobic sludge blanket reactor were related to response to stress, viral processes and iron-sulfur metabolism. Methanogenesis-related functions were most abundant in upflow anaerobic sludge blanket reactor. Effluent from tubular photobioreactor presented high abundances of functions related to nitrogen utilization, metal ion binding and antibiotic biosynthetic processes. Interestingly, the abundance of sequences related to ‘pathogenesis’ decreased from influent BW to SP1 to effluent from tubular photobioreactor. Our wastewater treatment system also decreased potential microbial functions related to pathogenesis. Conclusions: The new sanitation system studied here fosters microbial groups and functions that allow the system to efficiently and robustly recover carbon and nutrients while reducing pathogenic groups, ultimately generating a final effluent safe for discharge and reuse.

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Section: Discussionsupporting

confidence: 91%

On-Site Blackwater Treatment Fosters Microbial Groups and Functions to Efficiently and Robustly Recover Carbon and Nutrients

Kuramae

Dimitrov

Silva

et al. 2020

Preprint

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“…Natural antibiotics and other metabolites produced by microalgae and adhesion to the cell surface of microalgae aid in the reduction of pathogenic organisms [ 53 , 54 ]. The algal system could reduce pathogens to a non-detectable level within five days of processing, which may be attributed to the combination of low pH and other operating conditions such as high temperature, what findings are consistent with those of Delanka-Pedige [ 55 ]. The findings of this study contribute to understand the dynamics of the microbial community structure and functions in this on-site BW digestion system either at treatment scale or environmental scale.…”

Section: Discussionsupporting

confidence: 84%

On-Site Blackwater Treatment Fosters Microbial Groups and Functions to Efficiently and Robustly Recover Carbon and Nutrients

et al. 2020

View full text Add to dashboard Cite

Wastewater is considered a renewable resource water and energy. An advantage of decentralized sanitation systems is the separation of the blackwater (BW) stream, contaminated with human pathogens, from the remaining household water. However, the composition and functions of the microbial community in BW are not known. In this study, we used shotgun metagenomics to assess the dynamics of microbial community structure and function throughout a new BW anaerobic digestion system installed at The Netherlands Institute of Ecology. Samples from the influent (BW), primary effluent (anaerobic digested BW), sludge and final effluent of the pilot upflow anaerobic sludge blanket (UASB) reactor and microalgae pilot tubular photobioreactor (PBR) were analyzed. Our results showed a decrease in microbial richness and diversity followed by a decrease in functional complexity and co-occurrence along the different modules of the bioreactor. The microbial diversity and function decrease were reflected both changes in substrate composition and wash conditions. Our wastewater treatment system also decreased microbial functions related to pathogenesis. In summary, the new sanitation system studied here fosters microbial groups and functions that allow the system to efficiently and robustly recover carbon and nutrients while reducing pathogenic groups, ultimately generating a final effluent safe for discharge and reuse.

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“…Gross and Schnarrenberger reported in 1995 that strains of this Rhodophyta (red algae) species are able to grow mixotrophically and heterotrophically on 27 different sugars and sugar alcohols . Galdieria sulphuraria is able to grow not only in neutral environments but also in highly acidic environments, down to pH 1.8 , and G. sulphuraria is able to acidify its environment by an active proton efflux, thus reducing the costs of pH control and the risk of contamination .…”

Section: Extremophile Microalgae — Specialists For Harsh Processing Cmentioning

confidence: 99%

Microalgae wastewater treatment: Biological and technological approaches

Wollmann

Dietze

Ackermann

et al. 2019

Engineering in Life Sciences

263

108

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Current global environmental issues raise unavoidable challenges for our use of natural resources. Supplying the human population with clean water is becoming a global problem. Numerous organic and inorganic impurities in municipal, industrial, and agricultural waters, ranging from microplastics to high nutrient loads and heavy metals, endanger our nutrition and health. The development of efficient wastewater treatment technologies and circular economic approaches is thus becoming increasingly important. The biomass production of microalgae using industrial wastewater offers the possibility of recycling industrial residues to create new sources of raw materials for energy and material use. This review discusses algae‐based wastewater treatment technologies with a special focus on industrial wastewater sources, the potential of non‐conventional extremophilic (thermophilic, acidophilic, and psychrophilic) microalgae, and industrial algae‐wastewater treatment concepts that have already been put into practice.

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Pathogen reduction in an algal-based wastewater treatment system employing Galdieria sulphuraria

Cited by 77 publications

References 27 publications

On-Site Blackwater Treatment Fosters Microbial Groups and Functions to Efficiently and Robustly Recover Carbon and Nutrients

On-Site Blackwater Treatment Fosters Microbial Groups and Functions to Efficiently and Robustly Recover Carbon and Nutrients

On-Site Blackwater Treatment Fosters Microbial Groups and Functions to Efficiently and Robustly Recover Carbon and Nutrients

Microalgae wastewater treatment: Biological and technological approaches

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