Pathogen reduction through additive-free short-wave uv light irradiation retains the optimal efficacy of human platelet lysate for the expansion of human bone marrow mesenchymal stem cells

Viau, Sabrina; Chabrand, Lucie; Eap, Sandy; Lorant, Judith; Rouger, Karl; Goudaliez, F.; Sumian, Chryslain; Delorme, Bruno

doi:10.1016/j.jcyt.2017.02.287

Cited by 4 publications

(28 citation statements)

References 38 publications

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“…The expression of a panel of surface markers was assessed, following previously described protocols . For each antigen tested, 200,000 cells re‐suspended in cold PBS were incubated with the following phycoerythrin (PE)‐conjugated monoclonal antibody: CD13 (#555394), CD29 (#555443), CD34 (#345802), CD40 (#555589), CD44 (#555479), CD45 (#555483), CD49c (#556025), CD49e (#555617), CD51 (#FAB1219P), CD63 (#556020), CD73 (#550257), CD80 (#PN IM1976U), CD86 (#PN IM2729U), CD90 (#555596), CD105 (#560839), CD146 (#5050‐PE100T), or HLA‐DR (#PN IM0464U) at saturating concentration, for 30 minutes in the dark at +4°C.…”

Section: Methodsmentioning

confidence: 99%

“…Adipogenic and osteogenic differentiation capacity was assessed by seeding cells at 10,000 cell/cm 2 on an appropriate surface: 12‐well plates, 24‐well plates, and T25 flasks. Previously described protocols were followed . Adipogenic and osteogenic differentiations were induced using specific induction media …”

Section: Methodsmentioning

confidence: 99%

“…Previously described protocols were followed . Adipogenic and osteogenic differentiations were induced using specific induction media …”

Section: Methodsmentioning

confidence: 99%

“…After 14 days of culture, the adipogenic differentiation was revealed using Oil Red O staining, as previously described . Lipid droplets in the cytoplasm of the cells appeared to be stained red.…”

Section: Methodsmentioning

confidence: 99%

“…Performing the viral inactivation on the raw material, i.e., the platelet concentrates (PCs), would make it possible to use one of the viral inactivation technologies already described to inactivate viruses in PCs, namely: Intercept, Mirasol or THERAFLEX UV‐Platelets . Moreover, it has already been described that hPL prepared from THERAFLEX UV‐Platelets‐ or Intercept‐treated PCs is as efficient as non‐inactivated hPL to promote BM‐hMSC proliferation . Some viruses may be resistant to these technologies, for instance, HAV for Intercept and Mirasol and HIV‐1 for THERAFLEX UV‐Platelets .…”

mentioning

confidence: 99%

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Viral inactivation of human platelet lysate by gamma irradiation preserves its optimal efficiency in the expansion of human bone marrow mesenchymal stromal cells

et al. 2019

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BACKGROUND Human platelet lysate (hPL) represents a powerful medium supplement for human mesenchymal stromal cell (hMSC) expansion. The recently published general chapters of the Pharmacopeia require the addition of a step of viral inactivation during the production process of such raw biological material used for cell‐based medicinal products. STUDY DESIGN AND METHODS The ability of gamma irradiation to inactivate viruses from a panel representative of the virus diversity was evaluated. The impact of gamma irradiation on hPL composition and efficiency as a supplement for hMSC culture was evaluated. RESULTS An efficient inactivation of all the viruses tested was demonstrated, with the minimum reduction factors obtained being superior to 4.5 log10 for human immunodeficiency virus (HIV) and hepatitis A virus (HAV) and superior to 5 log10 for bovine viral diarrhea virus (BVDV), pseudorabies virus (PRV) and porcine parvovirus (PPV). The gamma irradiation did not affect the content in interesting biochemical factors for cell culture or in growth factors (GF), except to basic fibroblast GF (bFGF) whereas it highly impacted the contents in the factors involved in the coagulation cascade. Finally, gamma irradiated hPL remained as efficient as non‐irradiated hPL for the proliferation, clonogenic potential, differentiation potential, and immunosuppressive properties of hMSCs. CONCLUSION The feasibility of using gamma irradiation to efficiently inactivate viruses in hPL while maintaining its optimal efficacy as a supplement for hMSC expansion was demonstrated. Such an inactivated hPL represents a very attractive raw material for the efficient production of safe cellular therapy products.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Previously described protocols were followed . Adipogenic and osteogenic differentiations were induced using specific induction media …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%