Pathogenesis and Tissue Distribution of Avian Infectious Bronchitis Virus Isolate IRFIBV32 (793/B Serotype) in Experimentally Infected Broiler Chickens

Boroomand, Zahra; Asasi, Keramat; Mohammadi, Ali

doi:10.1100/2012/402537

Cited by 30 publications

(33 citation statements)

References 23 publications

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“…In addition to the respiratory signs, gross lesions comprising of tracheal caseous exudate and plugs, and swollen kidneys (with or without) urate deposits were observed. Such clinical signs have been observed following infection with virulent IBV M41 (Butcher et al, 1990), Moroccan G strain (Ambali and Jones, 1990), 793B (Boroomand et al, 2012), It-02 (Dolz et al, 2012) and QX (Ganapathy et al, 2012). These clinical signs and lesions were more severe in SPF than in the broiler chicks, indicating differences in the susceptibility to IBV infection in these different types of birds.…”

Section: Discussionmentioning

confidence: 85%

“…In the Middle East and North Africa, the circulation of Massachusetts, 793B, QX, Dutch strains (Abdel-Moneim et al, 2006;Bourogaa et al, 2009;Amin et al, 2012;Boroomand et al, 2012) and Q1 (Ganapathy et al, 2015) have been reported. In addition, an increasing number of strains closely related to IS/885/00 have been reported in Israel (Meir et al, 2004), Egypt (Abdel-Moneim et al, 2012), Iraq (Mahmood et al, 2011), Libya (Awad et al, 2014a) and other Middle East countries (Ganapathy et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…The pathogenesis and host immune responses to several IBV strains are known, such as those of M41 (Crinion and Hofstad, 1972;Butcher et al, 1990), Beaudette (Geilhausen et al, 1973), Australian T-strain (Chong and Apostolov, 1982;Ignjatovic et al, 2002), Moroccan G strain (El-Houadfi et al, 1986;Ambali and Jones, 1990), 793B (Dhinakar Raj and Jones, 1996;Boroomand et al, 2012), QX (Wang et al, 1998;Terregino et al, 2008;Ganapathy et al, 2012), It-02 (Dolz et al, 2012), Q1 (Yu et al, 2001;Toffan et al, 2013) and more recently Brazilian IBV variant (USP-10 and USP-50) (Chacón et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Experimental infection of IS/885/00-like infectious bronchitis virus in specific pathogen free and commercial broiler chicks

Awad

Chhabra

Forrester

et al. 2016

Research in Veterinary Science

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Pathogenesis of an IS/885/00-like (IS/885) strain of variant infectious bronchitis virus (IBV) was examined in one day old specific pathogen free (SPF) and commercial broiler chicks. Chicks were humanely euthanized at 3, 6, 9, 12, 15, 21 and 28 days post infection (dpi) for necropsy examination, and tissues were collected for histopathology and virus detection by reverse transcription polymerase chain reaction (RT-PCR). Respiratory clinical signs and gross lesions consisting of tracheal caseous exudate and plugs, and swollen kidneys (with or without) urate deposits were observed in SPF and broiler chicks. The onset of disease developed more slowly and were of lesser severity in broiler compared to SPF chicks, reflecting the inhibitory effects of the IBV maternal-antibodies in the broiler chicks or genetic/strain susceptibility, or both. Head swelling was observed in one infected broiler chick at 15 dpi and the virus was recovered by RT-PCR and isolation. In the IS/885-infected SPF chicks, cystic oviducts were found in two female chicks. IS/885 was isolated from the cystic fluid. Using ELISA, low to moderate levels of the antibodies to IBV was detected in the SPF compared to broiler infected chicks.

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Section: Discussionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Experimental infection of IS/885/00-like infectious bronchitis virus in specific pathogen free and commercial broiler chicks

Awad

Chhabra

Forrester

et al. 2016

Research in Veterinary Science

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“…Although infectious bronchitis is initially a respiratory disease, viral titers are often lower in the trachea than in the enteric organs (Cavanagh and Gelb, 2008). Previous studies also reported a higher probability to detect IBV with the use of digestive tract samples than with respiratory samples (El-Houadfi et al, 1986;Almeida et al, 2012;Boroomand et al, 2012;Dolz et al, 2012). Therefore, an important recommendation would be to collect samples of at least these 2 systems (respiratory and digestive) to a more conclusive diagnosis of the IBV status in the flocks.…”

Section: Discussionmentioning

confidence: 99%

Infectious bronchitis virus in different avian physiological systems—A field study in Brazilian poultry flocks

et al. 2014

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Avian infectious bronchitis is a highly contagious viral disease with economic effects on poultry agribusiness. The disease presents multi-systemic clinical signs (respiratory, renal, enteric, and reproductive) and is caused by one coronavirus (infectious bronchitis virus, IBV). Infectious bronchitis virus is classified into different serotypes and genotypes (vaccine strains and field variants). This study aimed to evaluate the occurrence of IBV in commercial poultry flocks from 3 important producing regions in Brazil and to determine the tropism of the main circulating genotypes to 3 different avian physiological systems (respiratory, digestive, urinary/reproductive). Clinical samples with suggestive signs of IBV infection were collected from 432 different poultry commercial flocks (198 from broilers and 234 from breeders). The total number of biological samples consisted of organ pools from the 3 above physiological systems obtained of farms from 3 important producing regions: midwest, northeast, and south. Infectious bronchitis virus was detected by reverse-transcription, real-time PCR of the 5' untranslated region. The results showed 179 IBV-positive flocks (41.4% of the flocks), with 107 (24.8%) from broilers and 72 (16.8%) from breeders. There were similar frequencies of IBV-positive flocks in farms from different regions of the country, most often in broilers (average 54%) compared with breeders (average 30.8%). reverse-transcription was more frequently detected in the digestive system of breeders (40%), and in the digestive (43.5%) and respiratory (37.7%) systems of broilers. Infectious bronchitis virus genotyping was performed by a reverse-transcription nested PCR and sequencing of the S1 gene from a selection of 79 IBV-positive flocks (45 from broilers and 34 from breeders). The majority of the flocks were infected with Brazilian variant genotype than with Massachusetts vaccine genotype. These results demonstrate the predominance of the Brazilian variant (mainly in the enteric tract) in commercial poultry flocks from 3 important producing regions in Brazil.

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“…In years 2014-2015 genotypes variant-2, 4/91, IS/720, QX, IR-1 and Mass were isolated from broiler farms (Najafi et al, 2015). It was suggested in an experimental histological study that 793/B may be a nephropathogenic virus (Mahdavi et al, 2007;Boroomand et al, 2012). There is no information on the pathogenic traits of Iranian IBV genotypes.…”

Section: Discussionmentioning

confidence: 99%

Pathogenicity characteristics of an Iranian variant-2 (IS-1494) like infectious bronchitis virus in experimentally infected SPF chickens

Najafi¹,

Langeroudi²,

Hashemzadeh³

et al. 2016

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Summary. -Avian infectious bronchitis (IB) is a major cause of economic loss to the poultry industry. IB virus primarily affects respiratory tract, but strains differ in their tropism for such other target organs as kidneys and alimentary tract. The objective of this study was to estimate the pathogenicity of an Iranian IB virus (IBV) variant (variant-2) which is one of the most prevalent isolates circulating in Iranian poultry farms. SPF chickens were intranasally inoculated with 10 4 EID 50 /0.1 ml of the virus. Sera, fecal swabs, and different tissue samples were collected on different days post infection. Clinical signs, gross pathology, and histological changes were recorded. The amount of virus genome was quantified in different tissues and feces using quantitative real-time PCR assay. The highest viral loads were detected in the feces and cecal tonsils. Real-time PCR results demonstrated variant-2 tropism for respiratory tract, digestive system and renal tissue that is due to its epitheliotropic nature. This is the first pathogenicity study of Iranian variant-2 virus. Based on histology observations and clinical signs this isolate was classified as a nephropathogenic IBV. Further knowledge of IBV pathogenesis permits to perform more effective prevention practice.

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Pathogenesis and Tissue Distribution of Avian Infectious Bronchitis Virus Isolate IRFIBV32 (793/B Serotype) in Experimentally Infected Broiler Chickens

Cited by 30 publications

References 23 publications

Experimental infection of IS/885/00-like infectious bronchitis virus in specific pathogen free and commercial broiler chicks

Experimental infection of IS/885/00-like infectious bronchitis virus in specific pathogen free and commercial broiler chicks

Infectious bronchitis virus in different avian physiological systems—A field study in Brazilian poultry flocks

Pathogenicity characteristics of an Iranian variant-2 (IS-1494) like infectious bronchitis virus in experimentally infected SPF chickens

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