Pathogenic Bacterial Sensors Based on Carbohydrates as Sensing Elements

Liu, Haiying

doi:10.1007/978-0-387-75113-9_24

Cited by 2 publications

(2 citation statements)

References 192 publications

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Section: Resultsmentioning

confidence: 99%

“…Detection of pathogenic bacteria is critical for many applications in the food industry, in pharmaceutical manufacturing, for water and environmental quality control, and for clinical diagnosis and biosecurity purposes. 43 GCSPRI was used to detect representative pathogens: Escherichia coli, Listeria monocytogenes, and Bacillus globigii (as a surrogate for B. anthracis). Viable E. coli MC1061 (1 Â 10 8 CFU/mL in PBST) resulted in significant binding to goat polyclonal anti-E. coli antibody but not to the negative control ROIs on the same chip (Figure 3a).…”

Section: Detection Of Soluble Analytes; Toxins and Antibodiesmentioning

confidence: 99%

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A Microarray Biosensor for Multiplexed Detection of Microbes Using Grating-Coupled Surface Plasmon Resonance Imaging

Marusov¹,

Sweatt²,

Pietrosimone³

et al. 2011

Environ. Sci. Technol.

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Grating-coupled surface plasmon resonance imaging (GCSPRI) utilizes an optical diffraction grating embossed on a gold-coated sensor chip to couple collimated incident light into surface plasmons. The angle at which this coupling occurs is sensitive to the capture of analyte at the chip surface. This approach permits the use of disposable biosensor chips that can be mass-produced at low cost and spotted in microarray format to greatly increase multiplexing capabilities. The current GCSPRI instrument has the capacity to simultaneously measure binding at over 1000 unique, discrete regions of interest (ROIs) by utilizing a compact microarray of antibodies or other specific capture molecules immobilized on the sensor chip. In this report, we describe the use of GCSPRI to directly detect multiple analytes over a large dynamic range, including soluble protein toxins, bacterial cells, spores, and viruses, in near real-time. GCSPRI was used to detect a variety of agents that would be useful for diagnostic and environmental sensing purposes, including macromolecular antigens, a non-toxic form of Pseudomonas aeruginosa exotoxin A (ntPE), Bacillus globigii, Mycoplasma hyopneumoniae, Listeria monocytogenes, Escherichia coli, and M13 bacteriophage. These studies indicate that GCSPRI can be used to simultaneously assess the presence of toxins and pathogens, as well as quantify specific antibodies to environmental agents, in a rapid, label-free and highly multiplexed assay requiring nanoliter amounts of capture reagents.

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Section: Resultsmentioning

confidence: 99%

Section: Detection Of Soluble Analytes; Toxins and Antibodiesmentioning

confidence: 99%

A Microarray Biosensor for Multiplexed Detection of Microbes Using Grating-Coupled Surface Plasmon Resonance Imaging

Marusov¹,

Sweatt²,

Pietrosimone³

et al. 2011

Environ. Sci. Technol.

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Current and Future Trends in the Clinical Diagnosis of Rickettsioses Sensu Lato

Missailidis

Godoy²,

Mares-Guia³

et al. 2014

IJTDH

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Aims:The aim of this review is to present Rickettsioses sensu lato, with emphasis on their current and future clinical diagnosis. The review presents the conditions, the agents that cause them, and the current gold standards on their diagnosis in national and international reference centres. Additionally, this review covers the various emerging technologies available in the diagnosis of Rickettsioses and discusses their potential for future use as gold standards in the diagnosis of these diseases. Introduction: The introduction presents Rickettsioses sensu lato and gives a broad overview of the conditions they cause, the issues associated with their current diagnosis and the need for their improved, earlier and more accurate diagnosis, in order to prevent current issues with false negatives, misdiagnosis or delay in the diagnosis associated with these conditions, which often renders them grave or lethal. Main Body: The main body of the review presents in independent sections Rickettsias, Ehrlichia, Anaplasma, Bartonella and Coxiella and the conditions associated with each of these bacteria. Spotted fever, endemic typhus, human granulocytic anaplasmosis, human monocytic ehrlichioses, bartonellosis and Q-fever are some of the conditions associated with this group of proteobacteria. The emphasis is on the clinical diagnosis of these conditions and an overview of the current practice, gold standards in reference

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Pathogenic Bacterial Sensors Based on Carbohydrates as Sensing Elements

Cited by 2 publications

References 192 publications

A Microarray Biosensor for Multiplexed Detection of Microbes Using Grating-Coupled Surface Plasmon Resonance Imaging

A Microarray Biosensor for Multiplexed Detection of Microbes Using Grating-Coupled Surface Plasmon Resonance Imaging

Current and Future Trends in the Clinical Diagnosis of Rickettsioses Sensu Lato

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