Pathogenicity and its mode of action in different sedentary stages of Heterodera glycines (Tylenchida: Heteroderidae) by Verticillium lecanii hybrid strains

Abstract

The current study was conducted to investigate the pathogenicity and its mode of action by Verticillium lecanii hybrid strains in different sedentary stages of Heterodera glycines. Three different sedentary stages (pale yellow female, yellow brown cyst, and dark brown cyst) of H. glycines were treated with V. lecanii and incubated for 3 weeks on water agar. After 3 weeks of incubation, eggs were investigated for the following parameters: (i) the infection frequencies of eggs, (ii) the number of eggs laid, and (iii) the number of mature and healthy eggs. Subsequently, the fecundity of H. glycines treated with V. lecanii was investigated in greater detail. Consequently, some strains had a greater ability to infect eggs and this significantly reduced the number of eggs laid and mature eggs in pale yellow females. This study indicates that V. lecanii is more effective on females rather than cysts of H. glycines and that V. lecanii may act on H. glycines in multiple ways.

Potential of Lecanicillium spp. for management of insects, nematodes and plant diseases

“…Furthermore, this strain significantly reduced cyst and egg densities compared to the parental strains. Shinya et al (2008b) also evaluated the effects of fungal culture filtrates of the Lecanicillium hybrid strains on mature eggs, embryonated eggs (eggs fertilized but without development of juveniles), and J2 of SCN and compared these effects to those of their parental strains. The fungal culture filtrates of some hybrid strains inhibited egg hatch of mature eggs.…”

VALIDATION OF TECHNOLOGY COMPONENTS FOR PEANUT POD BORER (Etiella zinckenellaTriet.) CONTROL

“…The effectiveness of L. lecanii application caused by the conidia of the entomopathogenic fungus was able to infect variety of insect stages, starting from the egg, larvae and imago (Wang et al, 2007;Shinde et al, 2010;Park & Kim, 2010). The effication of L. lecanii also due to a wide range of hosts including the order; Homoptera, Hemiptera, Coleoptera, Lepidoptera; Orthoptera, nematodes and plant diseases (Gan et al, 2007;Goettel et al, 2008;Shinya et al, 2008). The advantages of L. lecanii was their ability to produce chitinase enzymes with high concentrations, these enzymes function as a degenerator of layers cuticle and toxic in killing host insects (Liu et al, 2003;Lu et al, 2005).…”

INTEGRATION OF BOTANICAL PESTICIDE AND ENTOMOPATHOGENIC FUNGI TO CONTROL THE BROWN STINK BUG Riptortus linearis F. (HEMIPTERA: ALYDIDAE) IN SOYBEAN

“…The evident of synergism between natural pesticides and entomopthogenic fungi L. lecanii was indicated as the increase in efficacy. It was found that the fungi and ASP, JSP acted as ovicidal on different insects species and nematodes (Shinya et al 2008a(Shinya et al & 2008b. Seed oil from both natural pesticides block natural micropyle that interfere on physiolocal process on egg development.…”