Pathogenicity of Clinical OXA-48 Isolates and Impact of the OXA-48 IncL Plasmid on Virulence and Bacterial Fitness

Hamprecht, Axel; Sommer, Julian; Willmann, Matthias; Brender, Christina; Stelzer, Yvonne; Krause, Felix F.; Tsvetkov, Tsvetan; Wild, Florian; Riedel-Christ, Sara; Kutschenreuter, Julia; Imirzalioglu, Can; Gonzaga, Aitor; Nübel, Ulrich; Göttig, Stephan

doi:10.3389/fmicb.2019.02509

Cited by 39 publications

(44 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This study is the first characterization of the carbapenemase OXA-484, a variant of the growing family of OXA-48-like enzymes. The dissemination of OXA-48-like carbapenemases has been attributed to highly efficient HGT to different species, low fitness burden of plasmids encoding bla OXA – 48 – like , association with epidemiological successful lineages and difficult to detect carbapenemase phenotypes ( Hamprecht et al, 2019 ; Pitout et al, 2019 ). The plasmid pOXA-484 from isolate EC-JS316 was transferable to E. coli and K. quasipneumoniae subsp.…”

Section: Discussionmentioning

confidence: 99%

“…Clinical E. coli and K. pneumoniae isolates encoding bla OXA – 48 , bla OXA – 181 , bla OXA – 232 , or bla OXA – 484 were employed as donors and sodium azide-resistant E. coli J53 and K. quasipneumoniae subsp. quasipneumoniae PRZ as recipients ( Hamprecht et al, 2019 ). Plasmid DNA from clinical isolates carrying bla OXA – 232 was extracted using the PureYield TM Plasmid Midiprep System (Promega, Walldorf, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…The rapid dissemination of OXA-48 has been attributed to efficient horizontal gene transfer (HGT) of plasmids harboring bla OXA – 48 and a low fitness burden by carriage of these plasmids ( Hamprecht et al, 2019 ). At least 35 variants of OXA-48, commonly referred to as OXA-48-like β-lactamases (e.g., OXA-181, OXA-232, OXA-244), have been reported ( Naas et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

OXA-484, an OXA-48-Type Carbapenem-Hydrolyzing Class D β-Lactamase From Escherichia coli

Sommer

Krause

et al. 2021

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

OXA-48-like carbapenemases are among the most frequent carbapenemases in Gram-negative Enterobacterales worldwide with the highest prevalence in the Middle East, North Africa and Europe. Here, we investigated the so far uncharacterized carbapenemase OXA-484 from a clinical E. coli isolate belonging to the high-risk clone ST410 regarding antibiotic resistance pattern, horizontal gene transfer (HGT) and genetic support. OXA-484 differs by the amino acid substitution 214G compared to the most closely related variants OXA-181 (214R) and OXA-232 (214S). The blaOXA–484 was carried on a self-transmissible 51.5 kb IncX3 plasmid (pOXA-484) showing high sequence similarity with plasmids harboring blaOXA–181. Intraspecies and intergenus HGT of pOXA-484 to different recipients occurred at low frequencies of 1.4 × 10–7 to 2.1 × 10–6. OXA-484 increased MICs of temocillin and carbapenems similar to OXA-232 and OXA-244, but lower compared with OXA-48 and OXA-181. Hence, OXA-484 combines properties of OXA-181-like plasmid support and transferability as well as β-lactamase activity of OXA-232.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

OXA-484, an OXA-48-Type Carbapenem-Hydrolyzing Class D β-Lactamase From Escherichia coli

Sommer

Krause

et al. 2021

Front. Microbiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…57 In the current study, the conjugation experiment for the bla OXA-48 harboring plasmids from representative isolates was successful consistent with other studies. [59][60][61] Based on our molecular typing results, both methods can be used for the determination of the clonal relationships of K. pneumoniae strains. Although ERIC-PCR is a more rapid and easy method compared to PFGE, its discriminatory power seemed to be less than that of PFGE.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Carbapenemase-Producing Klebsiella pneumoniae Isolated from Egyptian Pediatric Cancer Patients Including a Strain with a Rare Gene-Combination of β-Lactamases

Osama¹,

El-Mahallawy²,

Mansour³

et al. 2021

IDR

View full text Add to dashboard Cite

Purpose Healthcare-associated infections caused by multi-drug-resistant (MDR) pathogens are a global threat. We aim to assess the clonal relatedness among carbapenemase-producing Klebsiella pneumoniae (CPKP) strains infecting Egyptian pediatric cancer patients. Materials and Methods Identification and antimicrobial susceptibility testing of 149 Gram-negative isolates obtained from pediatric cancer patients were performed by VITEK 2. Genes encoding carbapenemases and extended-spectrum β-lactamases were detected by PCR and verified by DNA sequencing of representative samples. The transferability of the plasmids harboring bla OXA-48 , from representative clinical samples, was evaluated by performing a conjugation experiment followed by PCR and MIC shift determination. Clonal relationships among the bla OXA-48 -harboring K. pneumoniae isolates were determined by enterobacterial repetitive intergenic consensus (ERIC)-PCR and pulsed-field gel electrophoresis (PFGE). Results Carbapenem resistance was observed in 59% of the isolates. The most prevalent species was K. pneumoniae (45.6%) and 57% of them were isolated from ICU. Fifty-nine % of the K. pneumoniae isolates were carbapenemase-producers and bla OXA-48 was detected in (58%) of them. One isolate co-harbored bla OXA-48, bla NDM-1 , and bla IMP-1 genes for the first time in Egypt. PCR and meropenem MIC shift confirmed the success of the transferability of representative plasmids to E. coli K12. ERIC and PFGE identified 93% and 100% of the K. pneumoniae with a similarity coefficient ≥85%, respectively, including strains with indistinguishable patterns, suggesting possible clonal dissemination. Conclusion Our findings underline the dissemination of diverse clones of MDR CPKP among Egyptian pediatric cancer patients. Hence, routine molecular characterizations followed by strict implementation of infection control measures are crucial to tackling this threat.

show abstract

“…These observations support other studies reporting significant increases in carbapenemase-producing Enterobacteriaceae in East Africa, in spite of this presumably low selective pressure [ 69 , 88 ]. While a large number of plasmids carrying bla OXA-48 and related carbapenemase genes have few or no additional ARDs, many of these plasmids have minimal associated fitness cost and can be stably maintained in the absence of selective pressure [ 89 , 90 , 91 ]. Others have documented the maintenance of plasmids harboring larger numbers of ARDs in the absence of selective pressure [ 92 , 93 , 94 ].…”

Section: Resultsmentioning

confidence: 99%

Tracking Antimicrobial Resistance Determinants in Diarrheal Pathogens: A Cross-Institutional Pilot Study

Taitt

Łęski

Prouty

et al. 2020

IJMS

View full text Add to dashboard Cite

Infectious diarrhea affects over four billion individuals annually and causes over a million deaths each year. Though not typically prescribed for treatment of uncomplicated diarrheal disease, antimicrobials serve as a critical part of the armamentarium used to treat severe or persistent cases. Due to widespread over- and misuse of antimicrobials, there has been an alarming increase in global resistance, for which a standardized methodology for geographic surveillance would be highly beneficial. To demonstrate that a standardized methodology could be used to provide molecular surveillance of antimicrobial resistance (AMR) genes, we initiated a pilot study to test 130 diarrheal pathogens (Campylobacter spp., Escherichia coli, Salmonella, and Shigella spp.) from the USA, Peru, Egypt, Cambodia, and Kenya for the presence/absence of over 200 AMR determinants. We detected a total of 55 different determinants conferring resistance to ten different categories of antimicrobials: genes detected in ≥ 25 samples included blaTEM, tet(A), tet(B), mac(A), mac(B), aadA1/A2, strA, strB, sul1, sul2, qacEΔ1, cmr, and dfrA1. The number of determinants per strain ranged from none (several Campylobacter spp. strains) to sixteen, with isolates from Egypt harboring a wider variety and greater number of genes per isolate than other sites. Two samples harbored carbapenemase genes, blaOXA-48 or blaNDM. Genes conferring resistance to azithromycin (ere(A), mph(A)/mph(K), erm(B)), a first-line therapeutic for severe diarrhea, were detected in over 10% of all Enterobacteriaceae tested: these included >25% of the Enterobacteriaceae from Egypt and Kenya. Forty-six percent of the Egyptian Enterobacteriaceae harbored genes encoding CTX-M-1 or CTX-M-9 families of extended-spectrum β-lactamases. Overall, the data provide cross-comparable resistome information to establish regional trends in support of international surveillance activities and potentially guide geospatially informed medical care.

show abstract

Pathogenicity of Clinical OXA-48 Isolates and Impact of the OXA-48 IncL Plasmid on Virulence and Bacterial Fitness

Cited by 39 publications

References 38 publications

OXA-484, an OXA-48-Type Carbapenem-Hydrolyzing Class D β-Lactamase From Escherichia coli

OXA-484, an OXA-48-Type Carbapenem-Hydrolyzing Class D β-Lactamase From Escherichia coli

Molecular Characterization of Carbapenemase-Producing Klebsiella pneumoniae Isolated from Egyptian Pediatric Cancer Patients Including a Strain with a Rare Gene-Combination of β-Lactamases

Tracking Antimicrobial Resistance Determinants in Diarrheal Pathogens: A Cross-Institutional Pilot Study

Contact Info

Product

Resources

About