Pathogenicity of influenza virus.

Sweet, C.; Smith, H

doi:10.1128/mmbr.44.2.303-330.1980

Cited by 114 publications

(48 citation statements)

References 127 publications

(254 reference statements)

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“…Neutrophils are one of many types of innate immune cells that are recruited to sites of injury and infection upon acute inflammation (34)(35)(36). A typical pathological feature of fatal influenza virus infection is extensive neutrophil infiltration (37)(38)(39). Here, by using time-lapse imaging and in vivo staining of vascular neutrophils (24,25), we quantified the influx of neutrophils into infectious foci in mouse lungs.…”

Section: Discussionmentioning

confidence: 99%

In vivo imaging of the pathophysiological changes and neutrophil dynamics in influenza virus-infected mouse lungs

Ueki

Wang

Fukuyama

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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The pathophysiological changes that occur in lungs infected with influenza viruses are poorly understood. Here we established an in vivo imaging system that combines two-photon excitation microscopy and fluorescent influenza viruses of different pathogenicity. This approach allowed us to monitor and correlate several parameters and physiological changes including the spread of infection, pulmonary permeability, pulmonary perfusion speed, number of recruited neutrophils in infected lungs, and neutrophil motion in the lungs of live mice. Several physiological changes were larger and occurred earlier in mice infected with a highly pathogenic H5N1 influenza virus compared with those infected with a mouse-adapted human strain. These findings demonstrate the potential of our in vivo imaging system to provide novel information about the pathophysiological consequences of virus infections.

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Section: Discussionmentioning

confidence: 99%

In vivo imaging of the pathophysiological changes and neutrophil dynamics in influenza virus-infected mouse lungs

Ueki

Wang

Fukuyama

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

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“…12 We assessed the immunogenicity and protective efficacy of the plant-produced antigens in ferrets, the accepted and well-validated animal model for influenza. 19,23 It should be emphasized, however, that there is no prior report of immunizing animals with plant-produced recombinant influenza antigens. Therefore, we adopted an immunization regimen and route of administration that would allow us to assess whether the plantproduced influenza antigens are immunogenic.…”

Section: Immunogenicity and Protective Efficacy In Ferret Challenge Mmentioning

confidence: 99%

A plant‐produced influenza subunit vaccine protects ferrets against virus challenge

Mett

Musiychuk

et al. 2008

Influenza Resp Viruses

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Background Influenza A viruses are of major concern for public health, causing worldwide epidemics associated with high morbidity and mortality. Vaccines are critical for protection against influenza, but given the recent emergence of new strains with pandemic potential, and some limitations of the current production systems, there is a need for new approaches for vaccine development. Objective To demonstrate the immunogenicity and protective efficacy of plant‐produced influenza antigens. Method We engineered, using influenza A/Wyoming/3/03 (H3N2) as a model virus, the stem and globular domains of hemagglutinin (HA) produced in plants as fusions to a carrier protein and used purified antigens with and without adjuvant for ferret immunization. Results These plant‐produced antigens were highly immunogenic and conferred complete protection against infection in the ferret challenge model. The addition of plant‐produced neuraminidase was shown to enhance the immune response in ferrets. Conclusions Plants can be used as a production vehicle for vaccine development against influenza. Domains of HA can generate protective immune responses in ferrets.

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“…Several mechanisms are responsible for this effect on cells. 3,4,11,20,25,33,41,61,88,95,105,114,121,122,125,138 In some instances, cell metabolism is drastically altered. Macromolecular synthesis may be inhibited by a variety of mechanisms and may occur at the level of replication, transcription, or translation.…”

Section: Lytic Viral Infectionsmentioning

confidence: 99%