Pathogenicity Test of Bacterial and Fungal Fish Pathogens in Cirrihinus mrigala Infected with EUS Disease

Sharma, Parvati; Sihag, R. C.

doi:10.3923/pjbs.2013.1204.1207

Cited by 11 publications

(12 citation statements)

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“…In this research, we selected Salmonella enterica and Escherichia coli as Gram-negative indicator bacteria and Micrococcus luteus as Gram-positive indicator bacteria to identify the antimicrobial activity of LAB strains. Salmonella enterica is a common pathogenic bacteria to livestock, while the other two bacteria are also undesirable in silage, because their growth and proliferation not only consume nutriments, but may cause disease to animals ( Sharma and Sihag, 2013 ; Chaudhari and Kariyawasam, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Identification and Antimicrobial Activity Detection of Lactic Acid Bacteria Isolated from Corn Stover Silage

Li¹,

Ni²,

Pang

et al. 2015

Asian Australas. J. Anim. Sci

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A total of 59 lactic acid bacteria (LAB) strains were isolated from corn stover silage. According to phenotypic and chemotaxonomic characteristics, 16S ribosomal DNA (rDNA) sequences and recA gene polymerase chain reaction amplification, these LAB isolates were identified as five species: Lactobacillus (L.) plantarum subsp. plantarum, Pediococcus pentosaceus, Enterococcus mundtii, Weissella cibaria and Leuconostoc pseudomesenteroides, respectively. Those strains were also screened for antimicrobial activity using a dual-culture agar plate assay. Based on excluding the effects of organic acids and hydrogen peroxide, two L. plantarum subsp. plantarum strains ZZU 203 and 204, which strongly inhibited Salmonella enterica ATCC 43971T, Micrococcus luteus ATCC 4698T and Escherichia coli ATCC 11775T were selected for further research on sensitivity of the antimicrobial substance to heat, pH and protease. Cell-free culture supernatants of the two strains exhibited strong heat stability (60 min at 100°C), but the antimicrobial activity was eliminated after treatment at 121°C for 15 min. The antimicrobial substance remained active under acidic condition (pH 2.0 to 6.0), but became inactive under neutral and alkaline condition (pH 7.0 to 9.0). In addition, the antimicrobial activities of these two strains decreased remarkably after digestion by protease K. These results preliminarily suggest that the desirable antimicrobial activity of strains ZZU 203 and 204 is the result of the production of a bacteriocin-like substance, and these two strains with antimicrobial activity could be used as silage additives to inhibit proliferation of unwanted microorganism during ensiling and preserve nutrients of silage. The nature of the antimicrobial substances is being investigated in our laboratory.

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Section: Introductionmentioning

confidence: 99%

Identification and Antimicrobial Activity Detection of Lactic Acid Bacteria Isolated from Corn Stover Silage

Li¹,

Ni²,

Pang

et al. 2015

Asian Australas. J. Anim. Sci

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“…The conjugated α helical region of PspA to Vi enhanced protective immune response and provided protection against pneumococcal infection [14]. Antibody elicited by PspA recombinant protein and DNA vaccine proffer humoral response which is different from fragment crystallizable (Fc), (lgG1/lgG22 ratios) and fragment antigen-binding (Fab) epitopes of the induced antibodies [22]. The tissue culture lethal dose 50 (TCLD 50 ) determined by Cormier and Janes showed that zeolite could be used against hepatitis A virus infection [12].…”

Section: Discussionmentioning

confidence: 99%

Application of median lethal concentration (LC50) of pathogenic microorganisms and their antigens in vaccine development

Saganuwan

2020

BMC Res Notes

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Objective: Lack of ideal mathematical models to qualify and quantify both pathogenicity, and virulence is a dreadful setback in development of new antimicrobials and vaccines against resistance pathogenic microorganisms. Hence, the modified arithmetical formula of Reed and Muench has been integrated with other formulas and used to determine bacterial colony forming unit/viral concentration, virulence and immunogenicity. Results: Microorganisms' antigens tested are Staphylococcus aureus, Streptococcus pneumoniae, Pseudomonas aeruginosa in mice and rat, Edwardsiella ictaluri, Aeromonas hydrophila, Aeromonas veronii in fish, New Castle Disease virus in chicken, Sheep Pox virus, Foot-and-Mouth Disease virus and Hepatitis A virus in vitro, respectively. The LC 50 s for the pathogens using different routes of administrations are 1.93 × 10 3 (sheep poxvirus) and 1.75 × 10 10 for Staphylococcus aureus (ATCC29213) in rat, respectively. Titer index (TI) equals N log 10 LC 50 and provides protection against lethal dose in graded fashion which translates to protection index. N is the number of vaccine dose that could neutralize the LC 50. Hence, parasite inoculum of 10 3 to 10 11 may be used as basis for determination of LC 50 and median bacterial concentrations (BC 50).Pathogenic dose for immune stimulation should be sought at concentration about LC 10 .

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“…6-The antibacterial activity of essential oil in vitro: It was confirmed by determining basil essential oil on growing bacteria using plates incubated at 24h. for treatments and 48h for control according to Sharma and Sihag (2013).…”

Section: -Microbiological Analysismentioning

confidence: 99%

Evaluation of Bacteria Isolated from Olive Mill Wastewater as Plant Growth Promoter on Basil (Ocimum basilicum L.) plant

Ibrahim

2019

Alexandria Science Exchange Journal

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The aim of this study was evaluate Enterobacter asburiae as plant growth promoter, through a field experiment conducted at Baloza Research Station at Season 2017/2018 on the production of Basil (Ocimum basilicum L., Family: Lamiaceae). The results showed increasing in growth parameters and essential oil yield by Ent. asburiae treatment and Ent. asburiae + half dose of N2 treatment than the control. Ent. asburiae produced phytohormones and amino acids which increased plant growth parameters. The enhanced essential oil has antibacterial activity which showed as a zone of inhibition around bacterial growth on agar plates. Enterobacter asburiae produced ammonia which decreased fungi counts. The more positive response of the oils extracted of treated plant showed essential the presence of gram negative strains (Pseudomonas sp.) and gram positive strains (Listeria sp. and Bacillus cereus) by using Enterobacter asburiae treatment.

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Pathogenicity Test of Bacterial and Fungal Fish Pathogens in Cirrihinus mrigala Infected with EUS Disease

Cited by 11 publications

References 0 publications

Identification and Antimicrobial Activity Detection of Lactic Acid Bacteria Isolated from Corn Stover Silage

Identification and Antimicrobial Activity Detection of Lactic Acid Bacteria Isolated from Corn Stover Silage

Application of median lethal concentration (LC50) of pathogenic microorganisms and their antigens in vaccine development

Evaluation of Bacteria Isolated from Olive Mill Wastewater as Plant Growth Promoter on Basil (Ocimum basilicum L.) plant

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