Pathologic changes in cultured Nile tilapia (<i>Oreochromis niloticus</i>) associated with an outbreak of <i>Edwardsiella anguillarum</i>

Armwood, Abigail R.; Camus, Alvin C.; López-Porras, Adrián; Ware, Cyndi; Griffin, Matt J.; Soto, Esteban

doi:10.1111/jfd.13058

Cited by 21 publications

(18 citation statements)

References 33 publications

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“…The histopathological examination of the liver, spleen, and kidneys indicated that the bacterial infection of strain SNU2 induced similar pathological changes to that observed in a previous study performed on a Nile tilapia farm in Costa Rica (15). Most of the lesions were concentrated on the liver rather than the spleen or kidneys.…”

Section: Histopathological Analysissupporting

confidence: 79%

“…Moreover, multiple vacuoles were detected throughout the liver. Differences found, relative to past studies, include the signs of vasculitis causing bacteremia and heterophil infiltration, with melanomacrophage observed near bile duct (Figure 3D) (15). The spleen also showed similar signs of vasculitis and macrophage infiltration but the degree of severity was less that the liver.…”

Section: Histopathological Analysiscontrasting

confidence: 52%

“…), seabream species, channel catfish (Ictalurus punctatus), hybrid catfish (Ictalurus furcatus), and tilapia (Oreochromis sp.) (3,(14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%

“…These bacteria were originally isolated from diseased eels (Anguilla spp.) in China, and studies concerning the virulence of the bacterial isolates from a variety of fish species have been increasing in other countries, including China, Japan, USA, and Greece (3,4,15). In this study, we aim to identify the species of Edwardsiella isolated from infected Nile tilapia cultured in Korea; we additionally attempt to characterize the pathogenicity of the species identified, examine the damage to internal organs by histopathological examination, and determine antimicrobial susceptibility.…”

Section: Introductionmentioning

confidence: 99%

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Characterization and Pathological Analysis of a Virulent Edwardsiella anguillarum Strain Isolated From Nile Tilapia (Oreochromis niloticus) in Korea

Jun²,

Kim

et al. 2020

Front. Vet. Sci.

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Edwardsiella species are one of the top causative pathogens of mortality in various fisheries worldwide. Their role in zoonotic infections and increase in antibiotic-resistance has raised concerns and interests in many research fields. Similar to the studies investigating human clinical cases, there has been an increase in research examining the potential pathogenic role of the bacterium in aquaculture. Within the Edwardsiella family, Edwardsiella anguillarum was lastest group to be differentiated from the Edwardsiella tarda group, and many studies focusing on the virulence of this species have since ensued. In Korea, only E. tarda infections have been reported in aquaculture industries, and there have been no reports on economic losses incurred owing to E. anguillarum infection. There has been a recent report investigating the pathogenicity and pathological changes caused by E. anguillarum infection in a tilapia farm located in the Costa Rica. To the best of our knowledge, as ours is the first report of E. anguillarum infection in a Nile tilapia (Oreochromis niloticus) farm located in an Asian country, the pathogenicity of the bacterial strain was histopathologically compared to that of the past studies. As tilapia is one of the most globally consumed fish species, particularly throughout Asia, Europe, and America, an epidemiological study regarding the disease distribution is necessary for the control and prevention of the disease. Here, we report the first mass mortality case caused by E. anguillarum infection in a Nile tilapia farm located in Korea; the bacterial strain responsible was isolated, characterized, and pathologically analyzed.

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Section: Histopathological Analysissupporting

confidence: 79%

Section: Histopathological Analysiscontrasting

confidence: 52%

“…), seabream species, channel catfish (Ictalurus punctatus), hybrid catfish (Ictalurus furcatus), and tilapia (Oreochromis sp.) (3,(14)(15)(16).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characterization and Pathological Analysis of a Virulent Edwardsiella anguillarum Strain Isolated From Nile Tilapia (Oreochromis niloticus) in Korea

Jun²,

Kim

et al. 2020

Front. Vet. Sci.

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“…), eels ( Anguilla spp.) and catfish (Siluriformes) (Armwood et al, 2019; Griffin et al, 2016; Park et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Multiplex PCR assay for correct identification of the fish pathogenic species of Edwardsiella genus reveals the presence of E. anguillarum in South America in strains previously characterized as E. tarda

Costa

Chideroli

Lanes

et al. 2022

Journal of Applied Microbiology

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Aims Develop a species‐specific multiplex PCR to correctly identify Edwardsiella species in routine diagnostic for fish bacterial diseases. Methods and Results The genomes of 62 Edwardsiella spp. isolates available from the National Center for Biotechnology Information (NCBI) database were subjected to taxonomic and pan‐genomic analyses to identify unique regions that could be exploited by species‐specific PCR. The designed primers were tested against isolated Edwardsiella spp. strains, revealing errors in commercial biochemical tests for bacterial classification regarding Edwardsiella species. Conclusion Some of the genomes of Edwardsiella spp. in the NCBI platform were incorrectly classified, which can lead to errors in some research. A functional mPCR was developed to differentiate between phenotypically and genetically ambiguous Edwardsiella, with which, we detected the presence of Edwardsiella anguillarum affecting fish in Brazil. Significance and Impact of the Study This study shows that the misclassification of Edwardsiella spp in Brazil concealed the presence of E. anguillarum in South America. Also, this review of the taxonomic classification of the Edwardsiella genus is a contribution to the field to help researchers with their sequencing and identification of genomes, showing some misclassifications in online databases that must be corrected, as well as developing an easy assay to characterize Edwardsiella species in an end‐point mPCR.

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Susceptibility of Delta Select and Delta Control Channel Catfish lines to experimental Edwardsiella ictaluri and Edwardsiella piscicida infection

Bosworth,

Koshy,

Ware

et al. 2024

N American J Aquac

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ObjectiveThe Delta Select line of Channel Catfish Ictalurus punctatus was established in 2006 and has undergone five generations of genetic selection, specifically targeting increased growth rate and carcass yield, and was released to U.S. catfish farmers in 2020. While improvements in growth rate and carcass yield have been confirmed, performance for other important production traits has yet to be evaluated. Infectious disease, particularly bacterial infections caused by Edwardsiella spp., cause significant economic losses to catfish producers in the southeastern United States. Given the economic consequences of these bacterial pathogens, experimental infectivity trials were conducted comparing susceptibility to Edwardsiella spp. between the Delta Select line and a randomly bred line of Channel Catfish originating from the same base population (Delta Control line).MethodsTwenty‐six full‐sib families from each line were used in infectivity trials consisting of a high and a low dose of E. ictaluri (immersion challenge: high dose = 1.6 × 107 CFU/mL, low dose = 8.0 × 106 CFU/mL) and E. piscicida (intracoelomic challenge: high dose = 6.3 × 105 CFU/fish, low dose = 1.9 × 105 CFU/fish).ResultThe high challenge dose resulted in the highest mortality for both pathogens (p < 0.001), regardless of selection line. There was no significant difference in cumulative percent mortality between the Delta Select and Delta Control lines following the E. ictaluri challenge at either dose (p = 0.55). Delta Selects had reduced mortality (p < 0.001) in response to E. piscicida challenge compared with the Delta Controls, regardless of dose.ConclusionThese results indicate that selection for increased growth rate and carcass yield in the Delta Select Channel Catfish line did not negatively impact resistance to E. ictaluri while potentially improving resistance to E. piscicida.

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Pathologic changes in cultured Nile tilapia (Oreochromis niloticus) associated with an outbreak of Edwardsiella anguillarum

Cited by 21 publications

References 33 publications

Characterization and Pathological Analysis of a Virulent Edwardsiella anguillarum Strain Isolated From Nile Tilapia (Oreochromis niloticus) in Korea

Characterization and Pathological Analysis of a Virulent Edwardsiella anguillarum Strain Isolated From Nile Tilapia (Oreochromis niloticus) in Korea

Multiplex PCR assay for correct identification of the fish pathogenic species of Edwardsiella genus reveals the presence of E. anguillarum in South America in strains previously characterized as E. tarda

Susceptibility of Delta Select and Delta Control Channel Catfish lines to experimental Edwardsiella ictaluri and Edwardsiella piscicida infection

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