Pathological Seminal Plasma Proteins by Cellulose Acetate Electrophoresis

Clavert, A.; Montagnon, D.; Brun, Bernard

doi:10.3109/01485018108987346

Cited by 9 publications

(3 citation statements)

References 6 publications

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“…The first problem in studying vesicular secretion is to obtain pure samples. Several methods can be envisaged for this: puncture of seminal vesicles removed by surgery, use of the technique of split ejaculation in which the secretion of different adnexal glands may be separated, and lastly, study of ,,incomplete" seminal fluid in which the secretion of one type of adnexal gland is considerably reduced or absent (Clavert et al, 1981).…”

Section: Introductionmentioning

confidence: 99%

Secretory proteins of human seminal vesicles and their relationship to lipids and sugars: Sekretorische Proteine der menschlichen Bläschendrüse und ihre Beziehungen zu Lipiden und Zuckern

Montagnon¹,

Valtat²,

Vignon³

et al. 2009

Andrologia

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Proteins secreted by human seminal vesicles are strongly positively charged. Cellulose acetate electrophoresis show how the presence of two protein bands of vesicular origin (N3 and N4). When studied on SDS-PAGE there are three main bands of molecular weight 67,45 and 40 kDa, respectively. These proteins may be separated by a chromatographic process using gel filtration on Sephadex G 25 M and ion exchange chromatography on CM and SP Sephadex C 50. Fructose, secreted by seminal vesicles, is excreted with specific proteins and these complexes take part to coagulum formation. During liquefaction glucose appears progressively and fructose is released from complexes with proteins. Interconversion processes that transform fructose into glucose, originate from prostatic secretion. In man, the liquefaction process seems to be not due to proteolysis, but by the way of other mechanisms that transform vesicular proteins of very high molecular weight into sub-units with lower molecular weights in the first minutes after ejaculation. In species other than man, i.e. lemurian, fructose takes part in the coagulation process with other components (albumin, ions, -SH groups). Cholesterol appears to be in relation with proteins which have high molecular weights. Half of phospholipid content of seminal plasma is probably free. Incubation of seminal plasma with spermatozoa show that these cells use triglycerides for their metabolism. The ratio between cholesterol and phospholipids is an important marker for the capacitation-decapacitation process. Zusammenfassung. Die von der menschlichen Blaschendruse sezernierten Proteine sind stark positiv geladen. Zelluloseacetat-Elektrophorese zeigt das Vorhandensein von zwei Banden vesikularer Herkunft (N3 und N4). Nach SDS-PAGE findet man drei Banden mit Molekulargewichten von 67, 45 und 40 kDa. Diese Proteine konnen chromatographisch durch Gelfiltration auf Sephadex G 25 M und Ionenaustausch-Chromatographie auf C M und SP Sephadex C 50 gereinigt werden. Fruktose, die von den Blaschendrusen sezerniert wird, wird zusammen mit spezifischen Proteinen ausgeschleust und diese Komplexe beteiligen sich an der Bildung des seminalen Coagulum. Wahrend der Verflussigung erscheint zunehmend Glukose, und Fruktose wird aus den Proteinkomplexen freigesetzt. Umwandlungsprozesse, die Fruktose in Glukose umbauen, werden durch das Prostatasekret veranlafit. Beim Menschen scheint die Verflussigung nicht durch Proteolyse, sondern durch andere Mechanismen, bei denen hochmolekulare Blaschendrusen-Proteine wenige Minuten nach der Ejakulation in niedrige molekulare Untereinheiten zerlegt werden, 194 D. Montagnon et al. abzulaufen. In anderen Species als dem Menschen, z.B. hei Lemuren, nimmt Fruktose zusammen mit anderen Komponenten (Albumin, Ionen, -SH-Gruppen) am Koagulations-prozeR teil. Cholesterin scheint eine Beziehung zu hochmolekularen Proteinen zu haben. Ungefahr die Halfte des Phospholipids im Seminalplasma ist wahrscheinlich frei. Inkuhation von Seminalplasma mit Spermien zeigt, daR diese Zellen Triglyceride...

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Section: Introductionmentioning

confidence: 99%

Secretory proteins of human seminal vesicles and their relationship to lipids and sugars: Sekretorische Proteine der menschlichen Bläschendrüse und ihre Beziehungen zu Lipiden und Zuckern

Montagnon¹,

Valtat²,

Vignon³

et al. 2009

Andrologia

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“…Lyon, France). The tech nics and methods used for measurement have been described in a previous study [6], For the patients with a chronic infection of the urogenital tract, a study of the seminal plasma with measurement of the prostatic acid phosphatases, fructose and total proteins was carried out [9], The statistical analysis of the results was made by Student's t test.…”

Section: Methodsmentioning

confidence: 99%

Alterations in the Lipid Composition of Seminal Plasma in Patients with a Chronic Infection of the Urogenital Tract

Vignon

Clavert²,

Cranz³

et al. 1993

Urol Int

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The lipid composition of seminal plasma was studied in 15 control subjects and 21 patients consulting for hypofertility and showing a chronic infection of the urogenital tract. In the infected patients a significant reduction in total cholesterol, HDL cholesterol and total phospholipids was noted. Moreover, there is a significant correlation between the rates of total cholesterol and prostatic acid phosphatases and the rates of phospholipids and proteins in seminal plasma. Knowing the role of lipids in the phenomena of maturation and capacitation of spermatozoa, such modifications enable us to understand better the functional anomalies of sperm observed in patients with chronic infection of the urogenital tract and also enable us to explain the effects of the infection on fertility.

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“…Figura 32 -Gráfico do pH durante a reação da PLA 2 e seu substrato 73 As glândulas vesiculares são responsáveis pela secreção de líquido seminal que tem como função nutrir gametas, manter o pH seminal, fonte de energia para o espermatozóide pelo seu grande conteúdo de frutose (CLAVERT et al, 1981). Já o plasma seminal é um meio complexo, contendo vários produtos bioquímicos originados dos ductos deferentes, vesículas seminais, próstata, glândulas bulbouretrais e glândula de Litre (CHEMINEAU et al, 1991).…”

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Purificação da fosfolipase A2 e análise bioquímica do plasma seminal de ovinos e caprinos

Franco¹

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Pathological Seminal Plasma Proteins by Cellulose Acetate Electrophoresis

Cited by 9 publications

References 6 publications

Secretory proteins of human seminal vesicles and their relationship to lipids and sugars: Sekretorische Proteine der menschlichen Bläschendrüse und ihre Beziehungen zu Lipiden und Zuckern

Secretory proteins of human seminal vesicles and their relationship to lipids and sugars: Sekretorische Proteine der menschlichen Bläschendrüse und ihre Beziehungen zu Lipiden und Zuckern

Alterations in the Lipid Composition of Seminal Plasma in Patients with a Chronic Infection of the Urogenital Tract

Purificação da fosfolipase A2 e análise bioquímica do plasma seminal de ovinos e caprinos

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