2017
DOI: 10.1016/j.ymben.2017.08.001
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Pathway optimization and key enzyme evolution of N-acetylneuraminate biosynthesis using an in vivo aptazyme-based biosensor

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Cited by 49 publications
(30 citation statements)
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“…It has been reported that sgRNAs targeting different positions of a gene can produce different repressive strengths (Qi et al, 2013). This trait has been used in the construction of several microbial cell factories (Wang et al, 2017;J. Wu et al, 2017;Wu et al, 2015).…”
Section: Combination and Optimization Of Sgrnasmentioning
confidence: 99%
“…It has been reported that sgRNAs targeting different positions of a gene can produce different repressive strengths (Qi et al, 2013). This trait has been used in the construction of several microbial cell factories (Wang et al, 2017;J. Wu et al, 2017;Wu et al, 2015).…”
Section: Combination and Optimization Of Sgrnasmentioning
confidence: 99%
“…RedLibs [63] was recently developed; it is a tool that generates a library of RBS sequences for pathway flux optimization to reduce experimental trials and errors. Such models have been used in many studies to facilitate the construction and optimization of protein expression levels in synthetic pathways [64][65][66].…”
Section: Enzyme Gene Expression Optimizationmentioning
confidence: 99%
“…With the designed biosensor, the NeuAc biosynthesis pathway was optimized by RBS library screening and key enzyme evolution, which improved the titer by 34% and 23%, respectively. Finally, a mutant with 8.31 g/L NeuAc production was obtained after two-stage fermentation in minimal medium with the glucose as sole carbon source ( Yang et al, 2017 ).…”
Section: Industrial Application Of Biosensorsmentioning
confidence: 99%