Patient-derived models: Promising tools for accelerating the clinical translation of breast cancer research findings

Chen, Cao; Lu, Xin; Guo, Xinyan; Zhao, Huadong; Gao, Yuan

doi:10.1016/j.yexcr.2023.113538

Cited by 2 publications

(3 citation statements)

References 113 publications

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“…We first demonstrated that our GE blocks cell cycle at the G2/M phases, induces apoptosis, and reduces the invasiveness of MDA-MB-231 cells, in line with the effects described for individual garlic derivatives in this cell line [22,25]. We then investigated the effects of GE in four cell lines established from patient-derived xenografts (PDXs), preclinical models that maintain the characteristics of primary tumors in terms of the intraphenotypic heterogeneity of tumor cell populations [33][34][35]. We used the HBCX-17, T174, HBCX-9, and HBCX-39 cell lines, which, despite their common basal-like phenotype, belong to different TNBC molecular subtypes, according to the last Lehmann classification, also considering the response to neoadjuvant chemotherapy and disease progression [9,44].…”

Section: Discussionmentioning

confidence: 69%

“…Starting from the notion that TNBC shows intrinsic molecular heterogeneity, our study further aimed to analyze the role of GE on the cell cycle, morphology, and motility of breast cancer cells with a triple negative phenotype belonging to different molecular subtypes. With this purpose, the effects of GE were evaluated on four cell lines established from patient-derived xenografts (PDXs), translational preclinical models that better reflect the heterogeneity and diversity of tumor cell populations [33][34][35][36].…”

Section: Effects Of Garlic Extract On Growth and Invasive Potential O...mentioning

confidence: 99%

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Effects of Garlic on Breast Tumor Cells with a Triple Negative Phenotype: Peculiar Subtype-Dependent Down-Modulation of Akt Signaling

Brugnoli,

Dell’Aira,

Tedeschi

et al. 2024

Cells

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Breast cancer includes tumor subgroups with morphological, molecular, and clinical differences. Intrinsic heterogeneity especially characterizes breast tumors with a triple negative phenotype, often leading to the failure of even the most advanced therapeutic strategies. To improve breast cancer treatment, the use of natural agents to integrate conventional therapies is the subject of ever-increasing attention. In this context, garlic (Allium sativum) shows anti-cancerous potential, interfering with the proliferation, motility, and malignant progression of both non-invasive and invasive breast tumor cells. As heterogeneity could be at the basis of variable effects, the main objective of our study was to evaluate the anti-tumoral activity of a garlic extract in breast cancer cells with a triple negative phenotype. Established triple negative breast cancer (TNBC) cell lines from patient-derived xenografts (PDXs) were used, revealing subtype-dependent effects on morphology, cell cycle, and invasive potential, correlated with the peculiar down-modulation of Akt signaling, a crucial regulator in solid tumors. Our results first demonstrate that the effects of garlic on TNBC breast cancer are not unique and suggest that only more precise knowledge of the mechanisms activated by this natural compound in each tumor will allow for the inclusion of garlic in personalized therapeutic approaches to breast cancer.

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Section: Discussionmentioning

confidence: 69%

Section: Effects Of Garlic Extract On Growth and Invasive Potential O...mentioning

confidence: 99%

Effects of Garlic on Breast Tumor Cells with a Triple Negative Phenotype: Peculiar Subtype-Dependent Down-Modulation of Akt Signaling

Brugnoli,

Dell’Aira,

Tedeschi

et al. 2024

Cells

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“…Patient-derived organoid culture is also a promising model for cancer research, preserving the key biological characteristics of the original tumor while reducing time and cost, as well as improving success rates compared to PDX models and cell lines [62]. Patient-derived cancer organoids provide a closer reflection of the pathophysiological features of natural tumorigenesis and metastases than conventional cell culture or PDXs.…”

Section: Organoids or 3d Culturementioning

confidence: 99%

Challenges and Prospects of Patient-Derived Xenografts for Cancer Research

Jin,

Yoshimura,

Sewastjanow-Silva

et al. 2023

Cancers

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We discuss the importance of the in vivo models in elucidating cancer biology, focusing on the patient-derived xenograft (PDX) models, which are classic and standard functional in vivo platforms for preclinical evaluation. We provide an overview of the most representative models, including cell-derived xenografts (CDX), tumor and metastatic cell-derived xenografts, and PDX models utilizing humanized mice (HM). The orthotopic models, which could reproduce the cancer environment and its progression, similar to human tumors, are particularly common. The standard procedures and rationales of gastric adenocarcinoma (GAC) orthotopic models are addressed. Despite the significant advantages of the PDX models, such as recapitulating key features of human tumors and enabling drug testing in the in vivo context, some challenges must be acknowledged, including loss of heterogeneity, selection bias, clonal evolution, stroma replacement, tumor micro-environment (TME) changes, host cell carryover and contaminations, human-to-host cell oncogenic transformation, human and host viral infections, as well as limitations for immunologic research. To compensate for these limitations, other mouse models, such as syngeneic and humanized mouse models, are currently utilized. Overall, the PDX models represent a powerful tool in cancer research, providing critical insights into tumor biology and potential therapeutic targets, but their limitations and challenges must be carefully considered for their effective use. Lastly, we present an intronic quantitative PCR (qPCR) method to authenticate, detect, and quantify human/murine cells in cell lines and PDX samples.

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Patient-derived models: Promising tools for accelerating the clinical translation of breast cancer research findings

Cited by 2 publications

References 113 publications

Effects of Garlic on Breast Tumor Cells with a Triple Negative Phenotype: Peculiar Subtype-Dependent Down-Modulation of Akt Signaling

Effects of Garlic on Breast Tumor Cells with a Triple Negative Phenotype: Peculiar Subtype-Dependent Down-Modulation of Akt Signaling

Challenges and Prospects of Patient-Derived Xenografts for Cancer Research

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