Patogenicidad de tres cepas de hongos entomopatógenos a adultos de Anastrepha obliqua (Macquart) (Diptera: Tephritidae) en condiciones de laboratorio.

Díaz-Ordaz, Noé Hernández; Pérez, N. Mayek; Toledo, Jorge

doi:10.21829/azm.2010.263796

Cited by 14 publications

(5 citation statements)

References 21 publications

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“…B. bassiana strain LPSC 1067 used in this study was isolated from an orthopteran insect. There are several studies showing that strains pathogenicity is variable and has a direct relationship with the insect species host that was originally isolated (Muñoz, De la Rosa, & Toledo, 2009, Hernández Díaz-Ordaz, Pérez, & Toledo, 2010.…”

Section: Discussionmentioning

confidence: 99%

Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae)

Allegrucci

Velázquez

Russo

et al. 2020

RBT

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Introduction: Entomopathogenic fungi have been used as biological control agents of insect pests and have demonstrated a promising ability to colonize as endophytes a wide range of plant species. Objective: The aims of this study were to evaluate, in pepper plants, the most effective Beauveria bassiana inoculation techniques. In order to evaluate the presence of natural endophytes and its interaction with B. bassiana. Also to assess the effect of B. bassiana on the aphid Myzus persicae pepper leaf feeding preference and growth. Methods: Three inoculation techniques were evaluated: leaf spraying, seed immersion and root dipping. The pepper plants were inoculated with a conidia suspension adjusted to 1×108 conidia/ml. The presence of B. bassiana in the seedlings was evaluated at 7, 15 and 28 days after inoculation. Results: We found significant statistical differences between inoculation techniques. Foliar spraying showed the highest B. bassiana colonization frequency. The natural endophytes presented higher presence in the treatments in which B. bassiana had a low presence. This might indicate the occurrence of antifungal activity that interferes with the establishment of the entomopathogen in pepper tissues. We did not find statistical differences between inoculated and control pepper leaves with regard to the mortality and reproduction of the aphid pest M. persicae. However, the inoculation with B. bassiana showed a tendency to increase the mortality of adults and to decrease the offspring (nymphs). We did not find significant differences in the feeding preference between inoculated and control leaves, although there was a higher number of individuals on control leaves compared to those inoculated. Conclusions: The strain B. bassiana LPSC 1067 was established effectively as an endophyte in the pepper tissues. The most effective inoculation technique was leaf spraying. We did not find differences of statistical significance with regard to the mortality and reproduction of the aphid pest M. persicae. However, the inoculation with B. bassiana showed a tendency to increase the mortality of adults and to decrease the offspring (nymphs). Future studies evaluating the indirect effects of endophytic entomopathogens on both plants and herbivorous insects are needed to further explore their potential use in the management of insect pests.

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Section: Discussionmentioning

confidence: 99%

Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae)

Allegrucci

Velázquez

Russo

et al. 2020

RBT

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“…Te coverslip was mounted on a slide and stained with lactophenol blue. In each mounting, the reading consisted of 100 conidia [22]. Conidia were considered germinated when the germ tube reached the length of half of the conidium.…”

Section: Physiological Characterizationmentioning

confidence: 99%

In Vitro Biological Activity of Beauveria bassiana, Beauveria peruviensis, and Metarhizium sp. against Hypothenemus hampei (Coleoptera: Curculionidae)

Chuquibala-Checan,

Torres-de la Cruz,

Leiva

et al. 2023

International Journal of Agronomy

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Coffee (Coffea arabica) is the main commodity in Peru and is the economic support for thousands of small farmers. However, coffee production is affected by the coffee berry borer (Hypothenemus hampei). Currently, H. hampei is the most important pest in whole coffee-growing regions in Peru. This study aimed to evaluate in vitro biological activity of Beauveria bassiana, Beauveria peruviensis, and Metarhizium sp. against Hypothenemus hampei in two trials at different times. Conidia production, Conidia viability, and pathogenicity against H. hampei were evaluated at three concentrations (1 × 105, 1 × 107, and 1 × 109 conidia/mL−1). In addition, lethal times (LT50 and LT90) and lethal concentrations (LC50 and LC90) were calculated. There were significant differences in conidia production ( P < 0.001) and conidia viability ( P < 0.041). The highest conidia production and conidia viability were reached by B. bassiana and B. peruviensis, respectively. Likewise, there were differences in the pathogenicity of the strains in the two tests carried out (test 1: P < 0.0009 and test 2: P < 0.0001). The highest mortality occurred in the treatments of B. bassiana 1 × 109 conidia/mL−1, B. bassiana 1 × 107 conidia/mL−1, and B. peruviensis 1 × 109 conidia/mL−1. The treatments with lower LT50 and LT90 were B. bassiana 1 × 109 conidia/mL−1 and B. peruviensis 1 × 109 conidia/mL−1, and the strains with the lowest LC50 and LC90 were B. peruviensis and B. bassiana. The in vitro characteristics shown by B. bassiana and B. peruviensis conditions suggest they should be evaluated in the field to determine the capability of these strains to reduce populations of H. hampei.

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“…Já no presente trabalho, houve uma emergência de 23% de adultos de A. fraterculus em solo estéril tratado com M. anisopliae, comparado a 76% do tratamento testemunha. Embora esse resultado se aproxime do resultado encontrado porLezama-Gutiérrez et al (2000) em solo franco, ainda são necessários estudos com o isolado amazônico para verificar a redução da emergência de A. fraterculus em campo.O potencial de M. anisopliae para controle biológico de Anastrepha obliqua (Macquart, 1835) também foi avaliado em condições de laboratório no México(Díaz-Ordaz et al, 2010) e na Colômbia(Osorio-Fajardo & Canal, 2011). Nestes estudos, M. anisopliae causou 89,8% de mortalidade de adultos após contato direto com a suspensão de conídios(Díaz-Ordaz et al, 2010) e 34% de mortalidade de adultos emergidos de vermiculita tratada com suspensão de conídios(Osorio-Fajardo &…”

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Mortalidade de imaturos e adultos de Anastrepha fraterculus (Wiedemann) (Diptera: Tephritidae) causada por Metarhizium anisopliae (Metsch.) Sorokin (Hypocreales: Clavicipitaceae) em condições de laboratório

Silva

Sousa²,

Santos³

et al. 2022

RSD

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Anastrepha fraterculus (Wiedemann) (Diptera: Tephritidae) é uma espécie de mosca-das-frutas relevante do ponto de vista econômico e encontra-se amplamente distribuída no Brasil. O objetivo deste estudo foi avaliar a patogenicidade do isolado Metarhizium anisopliae (Metsch.) Sorokin (CPAFAP-3.8) contra imaturos e adultos de A. fraterculus em laboratório. Foram utilizados quatro tratamentos: isolado de M. anisopliae em solo estéril e em solo não estéril, e testemunha em solo estéril e em solo não estéril. Após a aplicação das suspensões de cada tratamento, larvas de 3° instar de A. fraterculus foram dispostas sobre o solo e mantidas em câmara do tipo B.O.D. à temperatura de 26 ± 2ºC, sem fotofase. Foi avaliada a emergência e a mortalidade dos adultos por 28 dias. Os resultados evidenciaram que a emergência dos adultos foi menor no tratamento com M. anisopliae em solo estéril (23%). Embora a emergência tenha sido alta no tratamento com M. anisopliae em solo não estéril (70%), a mortalidade dos adultos foi de 98,6%. A redução da sobrevivência dos adultos ocorreu mais rapidamente no tratamento com M. anisopliae em solo estéril, onde em 7 dias todos os adultos estavam mortos. No tratamento com M. anisopliae em solo não estéril, a sobrevivência caiu para 25,7% em 7 dias e para 14,2% em 10 dias. Nesse estudo ficou demonstrado que o isolado de M. anisopliae (CPAFAP-3.8) provoca mortalidade de imaturos e adultos de A. fraterculus sob condições controladas. Porém, ainda são necessários estudos de campo para avaliar o real potencial deste micro-organismo para o controle biológico da praga.

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Patogenicidad de tres cepas de hongos entomopatógenos a adultos de Anastrepha obliqua (Macquart) (Diptera: Tephritidae) en condiciones de laboratorio.

Cited by 14 publications

References 21 publications

Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae)

Establishment of the entomopathogenic fungus Beauveria bassiana as an endophyte in Capsicum annuum and its effects on the aphid pest Myzus persicae (Homoptera: Aphididae)

In Vitro Biological Activity of Beauveria bassiana, Beauveria peruviensis, and Metarhizium sp. against Hypothenemus hampei (Coleoptera: Curculionidae)

Mortalidade de imaturos e adultos de Anastrepha fraterculus (Wiedemann) (Diptera: Tephritidae) causada por Metarhizium anisopliae (Metsch.) Sorokin (Hypocreales: Clavicipitaceae) em condições de laboratório

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