Pattern of selected calcium-binding proteins in the vestibular nuclear complex of two rodent species

Abstract

The distribution of immunoreactivity to calbindin, calretinin, and parvalbumin in the vestibular nuclear complex and the adjacent nucleus prepositus hypoglossi was studied in rats and gerbils. The distribution of stained fibers was the same for both rodent species. All three calcium-binding proteins were present in vestibular afferents. However, none of the three proteins were present in all afferent fibers. Many fibers were labeled in the vestibular nerve and in fascicles of the descending vestibular nucleus, as well as ascending fibers in the superior vestibular nucleus and fibers directed to the medial vestibular nucleus. Labeled terminals were present in the medial vestibular nucleus, especially along the ventricular border, in the neuropil of the superior vestibular nucleus, and scattered in the descending and ventral portions of the lateral vestibular nucleus. Calbindin-and parvalbumin-positive terminals, but not calretininpositive terminals, were present in the neuropil of the dorsal lateral vestibular nucleus, especially surrounding the large neuronal somas. Some of these terminals are presumably from cerebellar Purkinje cells, which were also labeled by both antibodies. The pattern of parvalbumin immunoreactivity was slightly different from that of calbindin, indicating that parvalbumin might be contained in additional fibers.Some neurons in the vestibular nuclear complex were labeled with antibodies to calretinin, but few cells were stained with either calbindin or parvalbumin antibodies. The largest group of calretinin-positive cells was a cluster of small-to medium-sized neurons located in a densely stained mesh of dendrites and terminals in the medial vestibular nucleus, adjacent to the ventricular border. o 1996 Wiley-Liss, Inc. 11991) Different populations ofparvalbuminand calbindin-D28k inirnunoreactive neurons contain GABA and accumulate .jH-D-Aspartate in the dorsal horn of the rat spinal cord. J. Comp.Neurol. .7 24: 114-124.

Localization of parvalbumin, calretinin, and calbindin D-28k in identified extraocular motoneurons and internuclear neurons of the cat

“…Baskets of immunoreactive terminals appeared that surrounded the somata of vestibular neurons. Similar findings have also been found in the rat and gerbil (Kevetter, 1996). Possible sources of PV-IR fibers and terminals in the vestibular complex are Purkinje cells and primary vestibular afferents, which, as previously shown in the rat (Celio and Heizmann, 1981;Celio, 1990;Demêmes et al, 1993), also stained intensely against PV in the cat.…”

“…Possible sources of PV-IR fibers and terminals in the vestibular complex are Purkinje cells and primary vestibular afferents, which, as previously shown in the rat (Celio and Heizmann, 1981;Celio, 1990;Demêmes et al, 1993), also stained intensely against PV in the cat. CaBP-IR fibers and terminals in the vestibular complex were as abundant as those containing PV and may also originate from Purkinje cells (which were also CaBP-IR in the cat) and primary vestibular axons, similar to previous descriptions in other species (Baimbridge and Miller, 1982;García-Segura et al, 1984;Dechesne et al, 1988;Celio, 1990;Demêmes et al, 1991;Kevetter, 1996). However, CaBP-IR axons in the eighth nerve of the cat were not as numerous as those that contained PV.…”

“…PV-IR cells were found in both areas, although not in great numbers. Rather, CR-IR neurons characterized the medial vestibular nucleus, as occurs in the rat (Sans et al, 1995;Kevetter, 1996), guinea pig (Sans et al, 1995), and gerbil (Kevetter, 1996). Medial vestibular neurons may therefore represent the main origin of the CR-IR terminals found in the abducens nucleus.…”

Subacute stent thrombosis associated with a heparin‐coated stent and heparin‐induced thrombocytopenia

“…In situ hybridization and immunocytochemistry gave consistent results for the distribution of CaR and changes in its expression in a subset of pMVN neurons. Our observations for the later developmental stages confirm those of previous studies, which characterized CaR‐immunoreactive neurons in various adult rodents (Arai et al, 1991; Sans et al, 1995; Kevetter, 1996; Kevetter and Leonard, 1997). However, confocal microscopy analysis of double‐immunocytochemical labeling provided additional information about the development of labeled neurons and profiles.…”

“…In the mature VN of rodents, calbindin‐D28k (CaB), calretinin (CaR), and parvalbumin (PV) display different distributions in subpopulations of neurons and afferents. Two groups of CaR‐immunoreactive neurons have been identified in VN (Arai et al, 1991; Sans et al, 1995; Kevetter, 1996; Kevetter and Leonard, 1997). The larger population constitutes a group of small to medium neurons, densely packed in the pMVN adjacent to the fourth ventricle border.…”