Patterned Liposome–Polymer Composite Coatings

Abstract

Engineered biointerphases are crucial for many biomedical applications involving the interaction of (bio)materials with cell and tissue. Providing cues to support cell adhesion/proliferation in form of cargo delivery and/or by providing spatially controlled adhesive patches are important aspects. We demonstrate the assembly of composite films consisting of negatively charged liposomes, poly(dopamine) or cholesterolmodified poly(methacrylic acid) (PMAc) capping layers and (poly(L lysine)/PMAc)x terminating multilayers. We show that hepatocytes adhere to the assembled composite coatings. Their association with fluorescent lipids from the film varies depending on the capping layer and cell adhesion time and was suggested to be an energy dependent process. Further, the incorporation of TX into Ln reduced the number of adhering hepatocytes to the film without terminating multilayers. An exemplary composite coating is patterned and the subsequent site-selective adhesion of hepatocytes is shown. For the first time, composite coatings to be used for substrate-mediated drug delivery with control over the positioning of the adhering cells are shown.