Patterning neuronal and glia cells on light-assisted functionalised photoresists

Nicolau, Dan V.; Taguchi, Takahisa; Taniguchi, Hiroshi; Tanigawa, Hideo; Yoshikawa, Shinya

doi:10.1016/s0956-5663(98)00133-x

Cited by 49 publications

(35 citation statements)

References 32 publications

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“…Biosensors are among the most recent applications of photoresists. [503] The carboxyl groups of 697 were used to anchor peptides for specific cell-attachment or cell-detachment functions.…”

Section: Diazoquinones (Quinonediazides) Photoresistsmentioning

confidence: 99%

100 Years of the Wolff Rearrangement

2002

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The conversion of α‐diazo ketones into ketenes, and products derived therefrom, was discovered by Wolff in 1902. Major applications of the Wolff rearrangement, such as the Arndt−Eistert reaction (homologation of carboxylic acids) and the ring contraction of cyclic ketones, have been with us for some while. Nevertheless, substantial progress has been made recently. The reaction mechanism has been explored by means of matrix isolation, time‐resolved spectroscopy, and computation. Full retention of configuration of the migrating group has been established by using enantioselective chromatography. The Arndt−Eistert reaction has witnessed a renaissance in the field of natural products, in particular β‐amino acids and β‐peptides. Ring‐contracting Wolff rearrangements and ketene cycloadditions have been applied in syntheses of increasingly complex, biologically active target molecules. These accomplishments, as well as unsolved problems, are addressed in the present review. (© Wiley‐VCH Verlag GmbH, 69451 Weinheim, Germany, 2002)

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“…Biosensors are among the most recent applications of photoresists. [503] The carboxyl groups of 697 were used to anchor peptides for specific cell-attachment or cell-detachment functions.…”

Section: Diazoquinones (Quinonediazides) Photoresistsmentioning

confidence: 99%

100 Years of the Wolff Rearrangement

2002

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“…This control has been elusive, and network experimentation has largely relied on dissociated culture and spontaneous synapse formation. Attempts to control connectivity have involved surface micropatterning, 6,17,18,21,23 which restricts adhesion to fixed paths, but still relies on neurite growth to spontaneously form synapses randomly.…”

Section: Introductionmentioning

confidence: 99%

Robust Micromechanical Neurite Elicitation in Synapse-Competent Neurons Via Magnetic Bead Force Application

2005

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The ability to engineer living networks of interconnected neurons with specified connectivity would facilitate the study of synaptogenesis and information processing in the nervous system. Previously, we found that a neurite can be elicited from embryonic chick forebrain neurons by direct mechanical means using magnetic bead force application (MBFA); however, our previous studies and others focused on young, synapse-incompetent neurons. To address this issue, we tested cultures of embryonic chick forebrain neurons of varying age and found that neurites could be micromechanically elicited via MBFA at all ages tested, which ranged between 7 and 22 embryonic equivalent (EE) days (days in ovo plus days in vitro). The probability of neurite initiation was at least 40% for all ages, with a maximum of approximately 80% after 2-4 days in vitro, and a decrease to approximately 60% by day 10 in vitro. The force required to elicit a neurite was approximately 1500 pN with a minimum of approximately 700 pN at embryonic equivalent day 14. The probability of success was similar for two rates of force application (10 and 500 pN/s). Neurite initiation via micromechanical force is robust with respect to cell age, and micromechanical force can induce neurites in synapse-competent neurons.

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“…Therefore neuroscientists need to improve the neuron-adhesive character of electrodes and avoid neural adhesion around electrodes over longer periods of time. The generation of photoresist-based technologies from micro-electronics (CONEY et al, 1996;SINGHVI et al, 1994;CHANG et al, 1999) facilitates the preparation of chemical patterns of neuron-adhesive substances on micro-electrode devices (NICOLAU et al, 1999) and is a promising tool in the development of accurate positioning of neurons on top of microelectrodes.…”

Section: Introductionmentioning

confidence: 99%

Long-term adhesion and survival of dissociated cortical neurons on miniaturised chemical patterns

Ruardij¹,

Goedbloed²,

Rutten³

2003

Med. Biol. Eng. Comput.

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The influence of neuron-adhesive pattern geometry on long-term adhesion, survival and pattern compliance of cortical neuronal tissue was studied over a period of 15 days. The results are relevant for a successful, long-term integration of neuronal cells with electrodes from micro-electronic devices. Microwells (depth 0.5 microm), with diameters of 25, 50, 100 and 150 microm and spacing distances of 15, 30, 60 and 90 microm, were etched in a neuron-repellent fluorocarbon (FC) layer and coated with neuron-adhesive polyethylenimine (PEI). Results showed that adhesion, survival and compliance to the underlying patterns were geometry- and time-dependent. After 1 day, adhesion was inversely proportional to the diameter of the microwells, thus favouring the 25 microm microwells. However, adhesion was best on 50 microm microwells after 15 days. Survival of neurons was limited on 25 microm microwells (viability function V(D, T) was 0.08), as opposed to the better survival on 150 microm microwells (V(D, T) was 0.25) after 15 days. In summary, the study shows that the chemical patterns with microwells of 150 microm diameter (90 microm spacing gap) are most suitable for application on neuro-electronic devices owing to the better long-term survival and high pattern compliance of the neuronal cells.

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Patterning neuronal and glia cells on light-assisted functionalised photoresists

Cited by 49 publications

References 32 publications

100 Years of the Wolff Rearrangement

100 Years of the Wolff Rearrangement

Robust Micromechanical Neurite Elicitation in Synapse-Competent Neurons Via Magnetic Bead Force Application

Long-term adhesion and survival of dissociated cortical neurons on miniaturised chemical patterns

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