Patterns of dye coupling involving serotonergic neurons provide insights into the cellular organization of a central complex lineage of the embryonic grasshopper Schistocerca gregaria

Boyan, George; Niederleitner, Bertram

doi:10.1007/s00427-010-0348-y

Cited by 9 publications

(4 citation statements)

References 90 publications

(150 reference statements)

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“…Protocols for immunolabeling with primary and secondary antibodies, microscopy, and image processing were all as previously described (see Boyan and Williams 2004 , 2007 ; Boyan and Niederleitner 2011 ; Ehrhardt et al 2015 , 2016 ).…”

Section: Methodsmentioning

confidence: 99%

From bristle to brain: embryonic development of topographic projections from basiconic sensilla in the antennal nervous system of the locust Schistocerca gregaria

Boyan,

Ehrhardt

2024

Dev Genes Evol

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The antennal flagellum of the locust S. gregaria is an articulated structure bearing a spectrum of sensilla that responds to sensory stimuli. In this study, we focus on the basiconic-type bristles as a model for sensory system development in the antenna. At the end of embryogenesis, these bristles are found at fixed locations and then on only the most distal six articulations of the antenna. They are innervated by a dendrite from a sensory cell cluster in the underlying epithelium, with each cluster directing fused axons topographically to an antennal tract running to the brain. We employ confocal imaging and immunolabeling to (a) identify mitotically active sense organ precursors for sensory cell clusters in the most distal annuli of the early embryonic antenna; (b) observe the subsequent spatial appearance of their neuronal progeny; and (c) map the spatial and temporal organization of axon projections from such clusters into the antennal tracts. We show that early in embryogenesis, proliferative precursors are localized circumferentially within discrete epithelial domains of the flagellum. Progeny first appear distally at the antennal tip and then sequentially in a proximal direction so that sensory neuron populations are distributed in an age-dependent manner along the antenna. Autotracing reveals that axon fasciculation with a tract is also sequential and reflects the location and age of the cell cluster along the most distal annuli. Cell cluster location and bristle location are therefore represented topographically and temporally within the axon profile of the tract and its projection to the brain.

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Section: Methodsmentioning

confidence: 99%

From bristle to brain: embryonic development of topographic projections from basiconic sensilla in the antennal nervous system of the locust Schistocerca gregaria

Boyan,

Ehrhardt

2024

Dev Genes Evol

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Section: Methodsmentioning

confidence: 99%

Central projections from Johnston’s organ in the locust: Axogenesis and brain neuroarchitecture

Boyan,

Williams,

Ehrhardt

2023

Dev Genes Evol

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Johnston’s organ (Jo) acts as an antennal wind-sensitive and/or auditory organ across a spectrum of insect species and its axons universally project to the brain. In the locust, this pathway is already present at mid-embryogenesis but the process of fasciculation involved in its construction has not been investigated. Terminal projections into the fine neuropilar organization of the brain also remain unresolved, information essential not only for understanding the neural circuitry mediating Jo-mediated behavior but also for providing comparative data offering insights into its evolution. In our study here, we employ neuron-specific, axon-specific, and epithelial domain labels to show that the pathway to the brain of the locust is built in a stepwise manner during early embryogenesis as processes from Jo cell clusters in the pedicel fasciculate first with one another, and then with the two tracts constituting the pioneer axon scaffold of the antenna. A comparison of fasciculation patterns confirms that projections from cell clusters of Jo stereotypically associate with only one axon tract according to their location in the pedicellar epithelium, consistent with a topographic plan. At the molecular level, all neuronal elements of the Jo pathway to the brain express the lipocalin Lazarillo, a cell surface epitope that regulates axogenesis in the primary axon scaffold itself, and putatively during fasciculation of the Jo projections to the brain. Central projections from Jo first contact the primary axon scaffold of the deutocerebral brain at mid-embryogenesis, and in the adult traverse mechanosensory/motor neuropils similar to those in Drosophila. These axons then terminate among protocerebral commissures containing premotor interneurons known to regulate flight behavior.

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“…Protocols for immunolabeling with primary and secondary antibodies, microscopy and image processing were all as previously described (see Williams 2004, 2007;Boyan and Niederleitner 2011;Ehrhardt et al 2015Ehrhardt et al , 2016.…”

Section: Immunolabelingmentioning

confidence: 99%

From bristle to brain: embryonic development of labeled lines in the antennal nervous system of the locust Schistocerca gregaria

Boyan,

Ehrhardt

2024

Preprint

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The antennal flagellum of the locust S. gregaria is an articulated structure bearing a spectrum of sensory sensilla that encode environmental stimuli mediating adaptive behavior. In this study we focus on the basiconic-type bristles as a model for sensory system development in the antenna. At the end of embryogenesis these bristles are found at conserved locations on each apical segment of the flagellum, are innervated by a dendrite from a sensory cell cluster in the underlying epithelium with each cluster directing an axon topographically to an antennal tract and the brain. We employ confocal imaging and immunolabeling to (a) identify mitotically active sense organ precursors for sensory cell clusters in the most apical annuli of the early embryonic antenna; (b) follow the subsequent differentiation of their neuronal progeny; and (c) map the spatial and temporal organization of axonal projections into the antennal tracts. We show that early in embryogenesis proliferative precursors are localized circumferentially within discrete epithelial domains of the flagellum. Differentiation of their clonal progeny begins apically and proceeds in a basal direction along the antenna so that the oldest neuronal populations are near the antennal tip with progressively younger populations located more basally. Autotracing reveals that axon fasciculation with a tract is sequential and reflects the location and age of the cell cluster along the flagellum. Cell cluster location and bristle location on the cuticle are therefore encoded topographically and temporally within the axon profile of the tract and its projection to the brain.

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Patterns of dye coupling involving serotonergic neurons provide insights into the cellular organization of a central complex lineage of the embryonic grasshopper Schistocerca gregaria

Cited by 9 publications

References 90 publications

From bristle to brain: embryonic development of topographic projections from basiconic sensilla in the antennal nervous system of the locust Schistocerca gregaria

From bristle to brain: embryonic development of topographic projections from basiconic sensilla in the antennal nervous system of the locust Schistocerca gregaria

Central projections from Johnston’s organ in the locust: Axogenesis and brain neuroarchitecture

From bristle to brain: embryonic development of labeled lines in the antennal nervous system of the locust Schistocerca gregaria

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